• 【Ti40Cu38Zr10Pd12大块金属玻璃的体外生物相容性评估。】 复制标题 收藏 收藏
    DOI:10.1007/s10856-013-5041-z 复制DOI
    作者列表:Blanquer A,Pellicer E,Hynowska A,Barrios L,Ibáñez E,Baró MD,Sort J,Nogués C
    BACKGROUND & AIMS: :The use of biocompatible materials has attained an increasing importance for tissue regeneration and transplantation. The excellent mechanical and corrosion properties of Ti40Cu38Zr10Pd12 bulk metallic glass (BMG) turn it into a potential candidate for its use in orthopaedic implants. Before being considered as a biomaterial, some biological parameters must be taken into account. In this study,mouse preosteoblasts were cultured in the presence or absence of the alloy at different times (24 h, 7 and 21 days) and no differences in cell viability were detected.Moreover, cells were able to adhere to the alloy surface by establishing focal contacts, and displayed a flattened polygonal morphology. After 14 days in culture, differentiation into osteoblasts was observed. Besides, the amount of Cu ions released and their potential toxic effects were analyzed, showing that the amount of Cu released did not increase cell death. Finally, the low levels of inflammatory cytokines secreted by THP-1 differentiated macrophages exposed to the alloy suggest the absence of an immunogenic response to the alloy. In conclusion, in vitro studies indicate that the Ti40Cu38Zr10Pd12 BMG could be considered as a biomaterial to be used in orthopaedic implants.
    背景与目标: 生物相容性材料的使用对于组织再生和移植越来越重要。 Ti40Cu38Zr10Pd12大块金属玻璃(BMG)的出色的机械和耐腐蚀性能使其成为骨科植入物的潜在候选者。在被考虑为生物材料之前,必须考虑一些生物学参数。在这项研究中,在不同时间(24小时,7天和21天)在有或没有合金的情况下培养小鼠成骨细胞,没有发现细胞活力的差异。焦点接触,并显示出扁平的多边形形态。培养14天后,观察到分化为成骨细胞。此外,分析了铜离子的释放量及其潜在的毒性作用,表明铜离子的释放量不会增加细胞死亡。最后,暴露于合金的THP-1分化巨噬细胞分泌的炎性细胞因子水平低,表明对合金缺乏免疫原性应答。总之,体外研究表明,Ti40Cu38Zr10Pd12 BMG可被认为是用于整形外科植入物的生物材料。
  • 【载有ChABC的PLGA纳米颗粒:在脊髓损伤动物模型中对生物相容性,功能恢复和轴突再生的全面研究。】 复制标题 收藏 收藏
    DOI:10.1016/j.ijpharm.2020.119037 复制DOI
    作者列表:Azizi M,Farahmandghavi F,Joghataei MT,Zandi M,Imani M,Bakhtiari M,Omidian H
    BACKGROUND & AIMS: :Spinal Cord Injury (SCI) is one of the leading causes of physical disability. In this study, spherical PLGA nanoparticles (NPs) containing ChABC enzyme were manufactured and fully characterized for SCI therapy. The NPs were used in the rat's contused spinal cord to assess the functional improvement and scar digestion. Twenty-three adult male Wistar rats (275 ± 25 g) were assigned into four groups of control, sham, blank-treated particle, and ChABC-treated particle. Throughout the survey, the BBB scores were obtained for all the groups. Finally, the injured sections of animals were dissected, and histological studies were conducted using Luxol fast blue and Bielschowsky. The biocompatibility and non-toxicity effects of the NPs on olfactory ensheathing cells (OECs) were confirmed by the MTT test. The flow-cytometry revealed the purity of cultured OECs with p75+/GFAP+ at around 87.9 ± 2.4%. Animals in the control and the blank-treated groups exhibited significantly lower BBB scores compared with the ChABC-treated particle group. Histological results confirmed the induced contusion models in the injured site. Myelin was observed in the treated groups, especially when the ChABC-loaded nanoparticles were utilized. The immunohistochemistry results indicated the scar glial degradation in animals treated by the ChABC-loaded particles. According to this study, the loaded particles can potentially serve as a suitable candidate for spinal cord repair, functional recovery and axonal regeneration.
    背景与目标: :脊髓损伤(SCI)是身体残疾的主要原因之一。在这项研究中,包含ChABC酶的球形PLGA纳米颗粒(NPs)被制造出来并完全用于SCI治疗。 NP被用于大鼠挫伤性脊髓中以评估功能改善和疤痕消化。将二十三只成年雄性Wistar大鼠(275±25 g)分为对照组,假对照组,空白处理颗粒和ChABC处理颗粒四组。在整个调查过程中,所有组均获得了BBB分数。最后,解剖动物的受伤部分,并使用Luxol耐晒蓝和Bielschowsky进行组织学研究。通过MTT测试证实了NP对嗅觉鞘细胞(OEC)的生物相容性和无毒作用。流式细胞仪检测显示,p75 / GFAP培养的OEC纯度约为87.9±2.4%。与ChABC处理的颗粒组相比,对照组和空白处理组的动物的BBB得分明显降低。组织学结果证实了受伤部位的挫伤模型。在治疗组中观察到髓磷脂,尤其是当使用装载ChABC的纳米颗粒时。免疫组织化学结果表明,用负载ChABC的颗粒处理的动物中的瘢痕神经胶质细胞降解。根据这项研究,负载的粒子可以潜在地充当脊髓修复,功能恢复和轴突再生的合适人选。
  • 【取决于脱乙酰度的壳聚糖-羟基磷灰石薄膜生物相容性的体内研究。】 复制标题 收藏 收藏
    DOI:10.1002/jbm.a.35993 复制DOI
    作者列表:Jeong KJ,Song Y,Shin HR,Kim JE,Kim J,Sun F,Hwang DY,Lee J
    BACKGROUND & AIMS: :Chitosan, produced from chitin, is one of the polymers with promising applications in various fields. However, despite diverse research studies conducted on its biocompatibility, its uses are still limited. The main reason is the degree of deacetylation (DOD), which represents the proportion of deacetylated units in the polymer and is directly correlated with its biocompatibility property. In this article, the in vivo biocompatibility of three chitosan-hydroxyapatite composite films composed of chitosan with different DOD values was investigated by traditional biological protocols and novel optical spectroscopic analyses. The DOD of the chitosan obtained from three different manufacturers was estimated and calculated by Raman spectroscopy, Fourier transform infrared spectroscopy, and proton nuclear magnetic resonance spectroscopy. The chitosan with the higher DOD induced a higher incidence of inflammation in skin cells. The amino group density, biodegradability, and crystallinity of chitosan are the three possible factors that need to be considered when determining the biocompatibility of the films for in vivo application, as they led to complicated biological results, resulting in either better or worse inflammation even when using chitosan products with the same DOD. This basic study on the relationship between the DOD and inflammation is valuable for the development of further chitosan-based researches. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1637-1645, 2017.
    背景与目标: :几丁质是由几丁质生产的,是在各种领域中具有广阔应用前景的聚合物之一。然而,尽管对其生物相容性进行了各种研究,但其用途仍然受到限制。主要原因是脱乙酰基度(DOD),它代表聚合物中脱乙酰基单元的比例,并与其生物相容性直接相关。在本文中,通过传统的生物方案和新颖的光谱分析研究了三种具有不同DOD值的壳聚糖组成的壳聚糖-羟基磷灰石复合膜的体内生物相容性。通过拉曼光谱,傅立叶变换红外光谱和质子核磁共振光谱估算和计算了从三个不同制造商处获得的壳聚糖的DOD。具有较高DOD的壳聚糖在皮肤细胞中引起较高的炎症发生率。壳聚糖的氨基密度,生物降解性和结晶度是确定用于体内应用的薄膜的生物相容性时需要考虑的三个可能因素,因为它们导致复杂的生物学结果,甚至导致炎症的好坏使用具有相同DOD的壳聚糖产品。这项关于DOD与炎症之间关系的基础研究对于进一步基于壳聚糖的研究是有价值的。 ©2017 Wiley Periodicals,Inc.J Biomed Mater Res部分A:105A:1637-1645,2017。
  • 【原位酶交联明胶水凝胶的机械性能和生物相容性。】 复制标题 收藏 收藏
    DOI:10.5301/ijao.5000553 复制DOI
    作者列表:Alarake NZ,Frohberg P,Groth T,Pietzsch M
    BACKGROUND & AIMS: OBJECTIVES:Gelatin, a degraded collagen, has been widely used as a scaffolding material in tissue engineering applications. In this work, we aimed at the development of in situ, cross-linking, cytocompatible hydrogels by the use of transglutaminase as a cross-linker for potential application in the regeneration of tissues. METHODS:Hydrogels were prepared from gelatin of different concentrations and bloom degree (175 (G175) or 300 (G300) bloom gelatin) and cross-linked with various amounts of microbial transglutaminase (mTG) at 37°C. Mechanical properties and cross-linking degree were studied by rheology and swelling experiments. Four hydrogels with different stiffness were selected for studies with embedded human adipose-derived stem cells (hASCs). RESULTS:Hydrogels were obtained with storage modulus (G') values between 11 (±1) Pa and 1,800 (±200) Pa with gelation times between 80 (±6) and 450 (±36) seconds. G300 cross-linked gelatin hydrogels displayed higher gel stiffness, lower swelling ratio and gelled more rapidly compared to the hydrogels prepared from G175. Stiffer hydrogels (50 and 200 Pa) showed greater ability to support the proliferation of hASCs than softer ones (11 and 30 Pa). The highest cell proliferation was observed with a hydrogel of 200 Pa modulus. CONCLUSIONS:Overall, transglutaminase cross-linked gelatin hydrogels might be suitable as injectable hydrogels for the engineering of musculoskeletal and other types of connective tissues.
    背景与目标: 目的:明胶是一种降解的胶原蛋白,已被广泛用作组织工程应用中的支架材料。在这项工作中,我们旨在通过使用转谷氨酰胺酶作为交联剂开发原位,交联,细胞相容性水凝胶,以潜在地应用于组织再生。
    方法:由不同浓度和水华程度的明胶(175(G175)或300(G300)水华明胶)制备水凝胶,并在37°C下与各种量的微生物转谷氨酰胺酶(mTG)交联。通过流变学和溶胀实验研究了力学性能和交联度。选择了四种具有不同刚度的水凝胶用于嵌入式人脂肪衍生干细胞(hASC)的研究。
    结果:获得的水凝胶的储能模量(G')值为11(±1)Pa至1,800(±200)Pa,胶凝时间为80(±6)至450(±36)秒。与从G175制备的水凝胶相比,G300交联的明胶水凝胶显示出更高的凝胶刚度,更低的溶胀率和更快地凝胶化。较坚硬的水凝胶(50和200 Pa)显示出比较软的水凝胶(11和30 Pa)更大的支持hASCs增殖的能力。用200 Pa模量的水凝胶观察到最高的细胞增殖。
    结论:总体而言,转谷氨酰胺酶交联的明胶水凝胶可能适合作为注射水凝胶用于肌肉骨骼和其他类型结缔组织的工程改造。
  • 【水热处理形成的Ca-P化合物对Mg-3Al-1Zn-1.5Ca合金生物降解和生物相容性的影响;体外和体内评估。】 复制标题 收藏 收藏
    DOI:10.1038/s41598-017-00656-0 复制DOI
    作者列表:Kim YK,Jang YS,Lee YH,Yi HK,Bae TS,Lee MH
    BACKGROUND & AIMS: :Chemical combinations of Ca-P produced via plasma electrolytic oxidation (PEO) and a hydrothermal treatment were fabricated to improve the initial corrosion resistance and biocompatibility of a biodegradable Mg-3Al-1Zn-1.5Ca alloy. For the formation of an amorphous calcium phosphate composite layer on the surface of a magnesium alloy, a PEO layer composed of MgO and Mg3(PO4)2 was formed by PEO in electrolytes containing preliminary phosphate ions. During the second stage, a thick and dense Ca layer was formed by Ca electrodeposition after PEO. Finally, a hydrothermal treatment was carried out for chemical incorporation of P ions in the PEO layer and Ca ions in the electrodeposition layer. The amorphous calcium phosphate composite layer formed by the hydrothermal treatment enhanced osteoblast activity and reduced H2O2 production, which is a known stress indicator for cells. As a result of co-culturing osteoblast cells and RAW 264.7 cells, the formation of amorphous calcium phosphate increased osteoblast cell differentiation and decreased osteoclast cell differentiation. Implanting the alloy, which had an amorphous calcium phosphate composite layer that had been added through hydrothermal treatment, in the tibia of rats led to a reduction in initial biodegradation and promoted new bone formation.
    背景与目标: :制造了通过等离子电解氧化(PEO)和水热处理产生的Ca-P的化学组合,以改善可生物降解的Mg-3Al-1Zn-1.5Ca合金的初始耐腐蚀性和生物相容性。为了在镁合金的表面上形成非晶质磷酸钙复合层,在含有初级磷酸根离子的电解质中,通过PEO形成由MgO和Mg3(PO4)2构成的PEO层。在第二阶段中,PEO后通过电沉积Ca形成厚而致密的Ca层。最后,进行水热处理以化学结合PEO层中的P离子和电沉积层中的Ca离子。通过水热处理形成的无定形磷酸钙复合层增强了成骨细胞活性并减少了H2O2的产生,这是细胞已知的应激指标。共培养成骨细胞和RAW 264.7细胞的结果是,无定形磷酸钙的形成增加了成骨细胞的分化并降低了破骨细胞的分化。将具有通过水热处理添加的无定形磷酸钙复合层的合金植入大鼠胫骨,可减少初始生物降解并促进新骨的形成。
  • 【基质支持的可聚合膜模拟膜的体内生物相容性和稳定性。】 复制标题 收藏 收藏
    DOI:10.1016/j.biomaterials.2006.09.003 复制DOI
    作者列表:Wilson JT,Cui W,Sun XL,Tucker-Burden C,Weber CJ,Chaikof EL
    BACKGROUND & AIMS: :The cell membrane establishes an important paradigm for the molecular engineering of coatings for implantable devices because of its intrinsic biocompatibility and ability to act as a template for the assembly of diverse membrane-associated macromolecules. A stabilized membrane-mimetic film was assembled on alginate/Ca(2+) hydrogel microcapsules by in situ polymerization of an acrylate functionalized phospholipid. The phospholipid monomer was prepared as unilamellar vesicles and fused onto octadecyl chains that were components of an amphiphilic terpolymer anchored onto a polyelectrolyte multilayer (PEM) by electrostatic interactions. Microcapsules coated with a membrane-mimetic film were implanted into the peritoneal cavity of C57BL/6 mice, and the short-term biostability and biocompatibility of membrane-mimetic films assembled on two different alginate/poly(l-lysine) PEM cushions were compared. The nature of the underlying PEM support had a profound impact on the biocompatibility of the membrane-mimetic film, as the percentage of retrieved microcapsules completely overgrown with host cells shifted from 66+/-5.9% to less than 1% when modifications to the PEM were made. When assembled on the appropriate PEM support, biocompatibility of membrane-mimetic-coated microspheres was high wherein 87.5+/-5.7% of the implanted microspheres were retrieved 4 weeks after implantation and 92.6+/-6.4% of the retrieved capsules were free of cell adhesion or fibrotic overgrowth. Finally, 4 weeks after implantation, microspheres coated with a Texas red-labeled membrane-mimetic film were imaged with confocal microscopy and exhibited a uniform film around the periphery of the implant, indicating a high degree of film biostability. Hence, membrane-mimetic films provide a new route for generating robust, biocompatible, and biochemically heterogeneous coatings for implantable devices through molecular self-assembly.
    背景与目标: :由于其固有的生物相容性和充当各种膜相关大分子组装的模板的能力,细胞膜为可植入设备的涂层分子工程建立了重要的范例。通过丙烯酸酯官能化磷脂的原位聚合将稳定的膜模拟膜组装在藻酸盐/ Ca(2)水凝胶微胶囊上。将磷脂单体制备为单层囊泡,并融合到十八烷基链上,十八烷基链是通过静电相互作用锚定在聚电解质多层(PEM)上的两亲三元共聚物的组成部分。将涂有膜模拟膜的微胶囊植入C57BL / 6小鼠的腹膜腔中,并比较组装在两种不同藻酸盐/聚(1-赖氨酸)PEM垫上的膜模拟膜的短期生物稳定性和生物相容性。潜在的PEM载体的性质对模拟膜的生物相容性产生了深远的影响,因为当对PEM进行修饰时,完全被宿主细胞长满的回收微胶囊的百分比从66 /-5.9%变为小于1%。制成。当在适当的PEM支架上组装时,膜模拟包被的微球的生物相容性很高,其中植入后4周回收了87.5 /-5.7%的植入微球,而92.6 /-6.4%的回收胶囊没有细胞粘附或纤维化过度生长。最后,在植入后4周,用共聚焦显微镜对涂有德克萨斯红标记膜模拟膜的微球进行成像,并在植入物周围显示出均匀的膜,表明膜的生物稳定性很高。因此,模拟膜膜为通过分子自组装为植入式设备生成坚固,生物相容性和生化异构涂层提供了一条新途径。
  • 【基于鞘核醋酸纤维的静电纺丝支架对内皮细胞和血小板的体外生物相容性。】 复制标题 收藏 收藏
    DOI:10.1163/092050609X12559317149363 复制DOI
    作者列表:Rubenstein DA,Venkitachalam SM,Zamfir D,Wang F,Lu H,Frame MD,Yin W
    BACKGROUND & AIMS: :Typically, tissue-engineered scaffolds mimic the topographical properties of the native extracellular matrix. However, other physical properties, such as the scaffold mechanical stiffness, are not imitated. The purpose of this study was to fabricate scaffolds with improved mechanical properties and investigate their biocompatibility towards endothelial cells and platelets. To enhance mechanical properties, an electrospinning apparatus was developed that fabricates fibers with sheath-core morphologies. Different combinations of cellulose acetate and chitosan were chosen to modulate the mechanical properties of the formed fibers. We hypothesized that mechanically stiffer scaffolds would improve endothelial cell growth and that all scaffolds would be compatible towards endothelial cells and platelets. Endothelial cell-culture conditions were quantified up to 5 days. Migration onto scaffolds was monitored for 10 days. Platelet aggregation, antagonized by thrombin receptor agonist peptide 6, was measured after scaffold incubation. A platelet activation time-course was assessed with the modified prothrombinase assay. As scaffold mechanical stiffness increased, endothelial cell growth within and adhesion to and migration throughout the scaffolds was promoted. Also, scaffolds did not induce platelet aggregation or activation. These results indicate that the mechanical stiffness of engineered scaffolds regulates endothelial cell-culture parameters and that these sheath-core electrospun scaffolds are compatible towards endothelial cells and platelets.
    背景与目标: 通常,组织工程支架模拟天然细胞外基质的地形特征。但是,其他物理属性(如脚手架的机械刚度)并未被模仿。这项研究的目的是制造具有改善的机械性能的支架,并研究其对内皮细胞和血小板的生物相容性。为了增强机械性能,开发了一种电纺丝设备,该设备可制造具有皮芯形态的纤维。选择乙酸纤维素和壳聚糖的不同组合以调节形成的纤维的机械性能。我们假设机械上较硬的支架将改善内皮细胞的生长,并且所有支架都将与内皮细胞和血小板相容。量化内皮细胞培养条件长达5天。监测到支架上的迁移10天。支架孵育后,测量凝血酶受体激动剂肽6拮抗的血小板聚集。用改良的凝血酶原酶测定法评估血小板活化的时间过程。随着支架机械刚度的增加,促进了内皮细胞在支架内的生长以及在支架上的粘附和迁移。而且,支架不诱导血小板聚集或活化。这些结果表明工程支架的机械刚度调节内皮细胞培养参数,并且这些鞘芯电纺支架与内皮细胞和血小板相容。
  • 【人内皮细胞调节后,改善了小肠粘膜下层(SIS)的生物相容性。】 复制标题 收藏 收藏
    DOI:10.1016/s0142-9612(03)00552-0 复制DOI
    作者列表:Woods AM,Rodenberg EJ,Hiles MC,Pavalko FM
    BACKGROUND & AIMS: :Small intestinal submucosa (SIS) is a naturally occurring tissue matrix composed of extracellular matrix proteins and various growth factors. SIS is derived from the porcine jejunum and functions as a remodeling scaffold for tissue repair. While SIS has proven to be a useful biomaterial for implants in vivo, problems associated with endothelialization and thrombogenicity of SIS implants may limit its vascular utility. The goal of this study was to determine if the biological properties of SIS could be improved by growing human umbilical vein endothelial cells (HUVEC) on SIS and allowing these cells to deposit human basement membrane proteins on the porcine substrate to create what we have called "conditioned" SIS (c-SIS). Using an approach in which HUVEC were grown for 2 weeks on SIS and then removed via a technique that leaves behind an intact basement membrane, we hypothesized that the surface properties of SIS might be improved. We found that when re-seeded on c-SIS, HUVEC exhibited enhanced organization of cell junctions and had increased metabolic activity compared to cells on native SIS (n-SIS). Furthermore, HUVEC grown on c-SIS released lower amounts of the pro-inflammatory prostaglandin PGI2 into the media compared to cells grown on n-SIS. Additionally, we found that adhesion of resting or activated human platelets to c-SIS was significantly decreased compared to n-SIS suggesting that, in addition to improved cell growth characteristics, conditioning SIS with human basement membrane proteins might decrease its thrombogenic potential. In summary, conditioning of porcine SIS by human endothelial cells improves key biological properties of the material that may improve its usefulness as remodeling scaffold for tissue repair. Identification of critical modifications of SIS by human endothelial cells should help guide future efforts to develop more biocompatible vascular grafts.
    背景与目标: 小肠粘膜下层(SIS)是由细胞外基质蛋白和各种生长因子组成的天然组织基质。 SIS来源于猪空肠,并作为组织修复的重塑支架。尽管已证明SIS是体内植入物的有用生物材料,但与SIS植入物的内皮化和血栓形成有关的问题可能会限制其血管利用。这项研究的目的是确定SIS的生物学特性是否可以通过在SIS上生长人脐静脉内皮细胞(HUVEC)并使这些细胞在猪基质上沉积人基底膜蛋白来创造,从而创造出我们所谓的“条件” SIS(c-SIS)。我们假设使用HUVEC在SIS上生长2周,然后通过留下完整的基底膜的技术将其除去的方法,我们假设SIS的表面性能可能会得到改善。我们发现,当在c-SIS上重新播种时,与天然SIS(n-SIS)上的细胞相比,HUVEC表现出增强的细胞连接组织,并具有增加的代谢活性。此外,与在n-SIS上生长的细胞相比,在c-SIS上生长的HUVEC将较少量的促炎性前列腺素PGI2释放到培养基中。此外,我们发现与n-SIS相比,静止或激活的人类血小板对c-SIS的粘附力显着降低,这表明,除了改善细胞生长特性外,用人类基底膜蛋白调节SIS可能会降低其血栓形成潜能。总之,人内皮细胞对猪SIS的调节改善了该材料的关键生物学特性,可以提高其作为用于组织修复的重塑支架的有用性。人内皮细胞对SIS的关键修饰的鉴定应有助于指导未来开发更具生物相容性的血管移植物的努力。
  • 【聚(3-羟基丁酸酯-co-4-羟基丁酸酯)共聚物在成纤维细胞中的体外生物相容性评估。】 复制标题 收藏 收藏
    DOI:10.1002/jbm.a.31000 复制DOI
    作者列表:Siew EL,Rajab NF,Osman AB,Sudesh K,Inayat-Hussain SH
    BACKGROUND & AIMS: :Among the various biomaterials available for tissue engineering and therapeutic applications, microbial polyhydroxyalkanoates offer the most diverse range of thermal and mechanical properties. In this study, the biocompatibility of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB); containing 50 mol % of 4-hydroxybutyrate] copolymer produced by Delftia acidovorans was evaluated. The cytotoxicity, mode of cell death, and genotoxicity of P(3HB-co-4HB) extract against V79 and L929 fibroblast cells were assessed using MTT assay, acridine orange/propidium iodide staining, and alkaline comet assay, respectively. Our results demonstrate that P(3HB-co-4HB) treated on both cell lines were comparable with clinically-used Polyglactin 910, where more than 60% of viable cells were observed following 72-h treatment at 200 mg/mL. Further morphological investigation on the mode of cell death showed an increase in apoptotic cells in a time-dependent manner in both cell lines. On the other hand, P(3HB-co-4HB) at 200 mg/mL showed no genotoxic effects as determined by alkaline comet assay following 72-h treatment. In conclusion, our study indicated that P(3HB-co-4HB) compounds showed good biocompatibility in fibroblast cells suggesting that it has potential to be used for future medical applications.
    背景与目标: :在可用于组织工程和治疗应用的各种生物材料中,微生物聚羟基链烷酸酯具有最广泛的热和机械性能范围。在这项研究中,聚(3-羟基丁酸酯-co-4-羟基丁酸酯)[P(3HB-co-4HB);评价了由Delftia acidovorans生产的含有50mol%的4-羟基丁酸酯]共聚物的共聚物。使用MTT法,assay啶橙/碘化丙啶染色和碱彗星法分别评估了P(3HB-co-4HB)提取物对V79和L929成纤维细胞的细胞毒性,细胞死亡方式和遗传毒性。我们的结果表明,在两种细胞系上处理的P(3HB-co-4HB)与临床使用的Polyglactin 910相当,在200 mg / mL的72小时处理后观察到超过60%的活细胞。对细胞死亡方式的进一步形态学研究显示,两种细胞系中凋亡细胞均以时间依赖性方式增加。另一方面,200 mg / mL的P(3HB-co-4HB)在72小时的处理后通过碱彗星测定没有显示出遗传毒性作用。总之,我们的研究表明P(3HB-co-4HB)化合物在成纤维细胞中显示出良好的生物相容性,这表明它有潜力用于未来的医学应用。
  • 【蓖麻聚氨酯的三种不同化学成分的体内生物相容性。】 复制标题 收藏 收藏
    DOI:10.1002/jbm.a.10105 复制DOI
    作者列表:Barros VM,Rosa AL,Beloti MM,Chierice G
    BACKGROUND & AIMS: :Alteration in the chemical composition of a biomaterial may be undertaken to improve its biological properties. The aim of this work was to evaluate the biocompatibility of three chemical compositions of Ricinus communis polyurethane (RCP): RCPp (pure RCP), RCP + CaCO(3), and RCP +Ca(3)(PO(4))(2). RCP cylinders were surgically implanted in rabbit femurs. After 8, 12, and 16 weeks, the femurs were removed, fixed, sectioned, ground, and stained by Stevenel's blue/Alizarin red S for light microscopy and histomorphometry. The osseointegration and osseoconductivity were calculated by means of image analysis and the data were submitted to the Kruskal-Wallis test followed by Dunn's test. Osseointegration was already completed after 8 weeks on RCP + Ca(3)(PO(4))(2) because similar values were found from week 8 to 16, whereas it showed a time-dependent increase on RCPp and RCP +CaCO(3). The osseointegration was greater on RCP + Ca(3)(PO(4))(2) in all periods when compared with RCPp, and after 8 and 12 weeks when compared with RCP + CaCO(3). None of the RCP samples presented osseoconductivity. The present results showed that RCP blended with calcium phosphate improved the biocompatibility by both enhancing and accelerating its osseointegration. Based on the absence of osseoconductivity, RCP was considered to be a bioinert material.
    背景与目标: :可以通过改变生物材料的化学组成来改善其生物学特性。这项工作的目的是评估蓖麻聚氨酯(RCP)的三种化学成分的生物相容性:RCPp(纯RCP),RCP CaCO(3)和RCP Ca(3)(PO(4))(2)。将RCP圆柱体通过外科手术植入兔股骨中。 8、12和16周后,取股骨,固定,切片,磨碎,并用史蒂文尔氏蓝/茜素红S染色,以进行光学显微镜检查和组织形态学测定。通过图像分析计算骨整合和骨电导率,并将数据提交给Kruskal-Wallis检验,然后进行Dunn检验。在8周后,RCP Ca(3)(PO(4))(2)上的骨整合已经完成,因为从第8周到第16周发现了相似的值,而RCPp和RCP CaCO(3)随时间增加。与RCPp相比,RCP Ca(3)(PO(4))(2)在所有时期的骨整合都更大,与RCP CaCO(3)相比在8周和12周之后。没有RCP样品表现出骨电导率。目前的结果表明,RCP与磷酸钙共混可通过增强和加速其骨整合来改善生物相容性。基于不存在骨导电性,RCP被认为是一种生物惰性材料。
  • 【用谷胱甘肽或其氨基酸作为扩链剂制备的分段聚氨酯的HUVEC生物相容性和血小板活化作用。】 复制标题 收藏 收藏
    DOI:10.1080/09205063.2013.782804 复制DOI
    作者列表:Perales-Alcacio JL,Santa-Olalla Tapia J,Mojica-Cardoso C,Vargas-Coronado RF,Chan-Chan LH,Headen DM,García AJ,Cervantes-Uc JM,Cauich-Rodríguez JV
    BACKGROUND & AIMS: :Novel biodegradable segmented polyurethanes (SPUs) were synthesized with polycaprolactone diol, 4,4'-methylen bis (cyclohexyl isocyanate) (HMDI), and either L-glutathione or its constituent amino acids (L-glutamic acid, L-cysteine and glycine) as chain extenders. Fourier transform infrared spectroscopy analysis revealed the feasibility of obtaining polyurethanes through the presence of NH (Amide II), C-N, C-O, and C=O bands and the absence of NCO band. Differential scanning calorimetry and X-ray diffraction revealed that a semicrystalline polymer (T m = 42-52 °C; 2θ = 21.3° and 23°) was obtained in all cases, while dynamic mechanical analysis (DMA) revealed an amorphous phase (T g = -30 to -36 (o)C). These properties, in addition to their high molecular weight, led to high moduli and higher extensibilities when glycine and glutamic acid were used as chain extenders. Clotting times (Lee-White test) and activated partial thromboplastin time determined on these polyurethanes were longer than with glass. In addition, all synthesized SPU exhibited platelet activation indexes below the collagen type I positive control. Human umbilical vein endothelial cells viability was higher in SPUs containing either glycine or cysteine. The obtained results indicate that SPUs that use cysteine as chain extender are promising candidates for cardiovascular applications.
    背景与目标: :用聚己内酯二醇,4,4'-亚甲基双(环己基异氰酸酯)和L-谷胱甘肽或其组成氨基酸(L-谷氨酸,L-半胱氨酸和甘氨酸)合成新型可生物降解的分段聚氨酯(SPU) )作为扩链剂。傅里叶变换红外光谱分析揭示了通过存在NH(酰胺II),C-N,C-O和C = O谱带以及不存在NCO谱带获得聚氨酯的可行性。差示扫描量热法和X射线衍射表明,在所有情况下均获得半结晶聚合物(T m = 42-52°C;2θ= 21.3°和23°),而动态力学分析(DMA)显示出非晶相(T g = -30至-36(o)C)。当使用甘氨酸和谷氨酸作为扩链剂时,这些特性,除了分子量高之外,还导致了高模量和更高的延伸性。在这些聚氨酯上测定的凝结时间(李白试验)和活化的部分凝血活酶时间长于玻璃。另外,所有合成的SPU均显示出低于I型胶原阳性对照的血小板活化指数。在含有甘氨酸或半胱氨酸的SPU中,人脐静脉内皮细胞的活力较高。获得的结果表明,使用半胱氨酸作为扩链剂的SPU是有希望的心血管应用候选药物。
  • 【贻贝启发的聚乙二醇化碳纳米管:生物相容性评估和药物递送应用。】 复制标题 收藏 收藏
    DOI:10.1039/c6tx00094k 复制DOI
    作者列表:Xu H,Liu M,Lan M,Yuan H,Yu W,Tian J,Wan Q,Zhang X,Wei Y
    BACKGROUND & AIMS: :Carbon nanotubes (CNTs) have been widely examined for biomedical applications. However, surface functionalization of CNTs with polymers is often required to improve their application performance. To obtain these CNT-based polymer nanocomposites, surface-initiated polymerization strategies are generally adopted. However, all of these methods rely on the surface oxidation of CNTs, which is a rather complex and time-consuming procedure, and involves hazardous reagents. In this work, a facile and efficient bio-inspired strategy was developed for surface PEGylation of CNTs via a combination of mussel-inspired chemistry and the Michael addition reaction. The potential biomedical applications of these PEGylated CNTs were evaluated for intracellular delivery of a normally used anticancer drug (Doxorubicin hydrochloride). Two steps were involved in this strategy, which included the surface coating of CNTs with polydopamine (PDA) through self-polymerization of dopamine, and a Michael addition reaction between the PDA-coated CNTs (CNT-PDA) and amino-functionalized polymers, which were obtained by free radical polymerization using poly(ethylene glycol) methyl methacrylate and N-(3-aminopropyl) methacrylamide as monomers. Results suggested that these PEGylated CNTs are well dispersed in aqueous solution and showed improved biocompatibility toward cancer cells. On the other hand, we also demonstrated that DOX can be effectively loaded on these PEGylated CNTs and delivered into cells for cancer treatment. More importantly, this strategy can also be utilized for surface modification of many other materials with different polymers due to the strong and universal adhesion of PDA and designability of polymerization. Therefore, this method should be of great interest for the fabrication of multifunctional nanocomposites for biomedical applications.
    背景与目标: :碳纳米管(CNTs)已被广泛用于生物医学应用。然而,通常需要用聚合物对CNT进行表面官能化以改善其应用性能。为了获得这些基于CNT的聚合物纳米复合材料,通常采用表面引发的聚合策略。但是,所有这些方法都依赖于CNT的表面氧化,这是一个相当复杂且耗时的过程,并且涉及有害试剂。在这项工作中,通过贻贝启发式化学反应和迈克尔加成反应的结合,开发了一种简便有效的生物启发性策略,用于碳纳米管的表面聚乙二醇化。评估了这些PEG化CNT的潜在生物医学应用对正常使用的抗癌药物(盐酸阿霉素)的细胞内递送的作用。此策略涉及两个步骤,包括通过多巴胺的自聚合作用用聚多巴胺(PDA)对CNT进行表面涂覆,以及PDA涂覆的CNT(CNT-PDA)与氨基官能化聚合物之间的迈克尔加成反应,通过使用聚(乙二醇)甲基丙烯酸甲酯和N-(3-氨基丙基)甲基丙烯酰胺作为单体的自由基聚合反应获得。结果表明这些聚乙二醇化的碳纳米管很好地分散在水溶液中,并显示出对癌细胞的改善的生物相容性。另一方面,我们还证明了DOX可以有效地负载在这些PEG化的CNT上并传递到细胞中进行癌症治疗。更重要的是,由于PDA的牢固和通用粘合性以及聚合的可设计性,该策略还可用于使用其他不同聚合物对许多其他材料进行表面改性。因此,该方法对于生物医学应用的多功能纳米复合材料的制造应引起极大的兴趣。
  • 【用作纸浆封盖剂的树脂基材料的生物相容性。】 复制标题 收藏 收藏
    DOI:10.1111/j.1365-2591.2003.00702.x 复制DOI
    作者列表:Costa CA,Oliveira MF,Giro EM,Hebling J
    BACKGROUND & AIMS: AIM:To evaluate and compare the response of pulps of rats capped with resin-modified glass-ionomer cement (RMGIC) or self-etching adhesive system. METHODOLOGY:Class I cavities were prepared on the occlusal surface of 54 maxillary first molars of 27 rats. Pulp exposure was performed on the cavity floor. The following resin-based materials were applied as pulp-capping agents: G1, Clearfil Liner Bond 2V (CLB 2V; Kuraray Co., Japan); G2, Vitrebond (VIT; 3M/ESPE, USA). In group 3 (control group), a calcium hydroxide/saline paste (CH; Labsynth, Brazil) was used. The cavities were restored with amalgam. After 7, 30 and 60 days, the animals were sacrificed and the jaws were processed for microscopic evaluation. RESULTS:Despite the inflammatory response caused by the experimental and the control materials at 7 days, pulpal healing associated with calcified barrier formation was observed at 60 days following the pulp therapy. Both resin-based materials promoted a large zone of cell-rich fibrodentine matrix deposition on the pulp horn related to the pulp exposure site, which was larger to VIT than to CLB 2V specimens. Tertiary dentine underneath the fibrodentine matrix was deposited by a layer of elongated pulpal cells. The remaining pulpal tissue exhibited normal histological characteristics. In the control group, healing and dentine-bridge formation was observed at 30 days. Pulpal breakdown occurred only when bacterial infection occurred. CONCLUSION:Both experimental pulp-capping agents allowed pulpal healing characterized by cell-rich fibrodentine and tertiary dentine deposition as well as calcified barrier formation.
    背景与目标: 目的:评估和比较用树脂改性的玻璃离聚物水泥(RMGIC)或自蚀刻胶粘剂系统覆盖的大鼠牙髓的反应。
    方法:在27只大鼠的54颗上颌第一磨牙的咬合面上准备I级腔。在模腔地板上进行纸浆暴露。以下树脂基材料用作纸浆封盖剂:G1,Clearfil Liner Bond 2V(CLB 2V;日本可乐丽公司); G2,Vitrebond(VIT; 3M / ESPE,美国)。在第3组(对照组)中,使用氢氧化钙/盐浆(CH; Labsynth,Brazil)。用汞合金修复了蛀牙。在第7、30和60天后,处死动物并处理颌骨以进行显微镜评估。
    结果:尽管实验和对照材料在第7天引起炎症反应,但在牙髓治疗后60天观察到与钙化屏障形成有关的牙髓愈合。两种基于树脂的材料都在与牙髓暴露部位有关的牙髓角上促进了大面积的富含细胞的纤维状牙本质基质的沉积,这对VIT而言比对CLB 2V标本更大。纤维状牙本质基质下方的叔牙本质被一层细长的牙髓细胞沉积。剩余的牙髓组织表现出正常的组织学特征。在对照组中,在30天时观察到愈合和牙本质桥形成。仅当发生细菌感染时才发生牙髓衰竭。
    结论:这两种实验性牙髓封闭剂均能使牙髓愈合,其特征是富含细胞的纤齿素和叔牙本质沉积以及钙化的屏障形成。
  • 【施万氏细胞在生物相容性聚合物电纺纤维和溶液流延膜支架表面上的体外生物相容性。】 复制标题 收藏 收藏
    DOI:10.1021/bm061152a 复制DOI
    作者列表:Sangsanoh P,Waleetorncheepsawat S,Suwantong O,Wutticharoenmongkol P,Weeranantanapan O,Chuenjitbuntaworn B,Cheepsunthorn P,Pavasant P,Supaphol P
    BACKGROUND & AIMS: :The in vitro responses of Schwann cells (RT4-D6P2T, a schwannoma cell line derived from a chemically induced rat peripheral neurotumor) on various types of electrospun fibrous scaffolds of some commercially available biocompatible and biodegradable polymers, i.e., poly(3-hydroxybutyrate) (PHB), poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), polycaprolactone (PCL), poly(l-lactic acid) (PLLA), and chitosan (CS), were reported in comparison with those of the cells on corresponding solution-cast film scaffolds as well as on a tissue-culture polystyrene plate (TCPS), used as the positive control. At 24 h after cell seeding, the viability of the attached cells on the various substrates could be ranked as follows: PCL film > TCPS > PCL fibrous > PLLA fibrous > PHBV film > CS fibrous approximately CS film approximately PLLA film > PHB film > PHBV fibrous > PHB fibrous. At day 3 of cell culture, the viability of the proliferated cells on the various substrates could be ranked as follows: TCPS > PHBV film > PLLA film > PCL film > PLLA fibrous > PHB film approximately PCL fibrous > CS fibrous > CS film > PHB fibrous > PHBV fibrous. At approximately 8 h after cell seeding, the cells on the flat surfaces of all of the film scaffolds and that of the PCL nanofibrous scaffold appeared in their characteristic spindle shape, while those on the surfaces of the PHB, PHBV, and PLLA macrofibrous scaffolds also appeared in their characteristic spindle shape, but with the cells being able to penetrate to the inner side of the scaffolds.
    背景与目标: :雪旺氏细胞(RT4-D6P2T,一种从化学诱导的大鼠周围神经肿瘤衍生的神经鞘瘤细胞系)对某些类型的市售生物相容性和可生物降解聚合物,即聚(3-羟基丁酸)的电纺纤维支架的体外反应与(PHB),聚(3-羟基丁酸酯-co-3-羟基戊酸酯)(PHBV),聚己内酯(PCL),聚(l-乳酸)(PLLA)和壳聚糖(CS)进行了比较在相应溶液流延膜支架以及组织培养聚苯乙烯平板(TCPS)上的细胞作为阳性对照。细胞接种后24小时,可将附着在各个基质上的细胞的生存能力分级如下:PCL膜> TCPS> PCL纤维> PLLA纤维> PHBV膜> CS纤维大约CS膜大约PLLA膜> PHB膜> PHBV纤维> PHB纤维。在细胞培养的第3天,可将增殖后的细胞在各种底物上的生存能力分级如下:TCPS> PHBV膜> PLLA膜> PCL膜> PLLA纤维> PHB膜近似于PCL纤维> CS纤维> CS膜> PHB纤维> PHBV纤维。细胞接种后约8小时,所有薄膜支架和PCL纳米纤维支架的平坦表面上的细胞均呈现其特有的纺锤形,而PHB,PHBV和PLLA宏观纤维支架的表面上的细胞也呈特征纺锤形。它们以其特有的纺锤形出现,但细胞能够穿透到支架的内侧。
  • 【植入后修饰的胶原/透明质酸膜的生物相容性研究。】 复制标题 收藏 收藏
    DOI:10.1023/a:1020183322002 复制DOI
    作者列表:Koller J,Bakos D,Sadlonová I I
    BACKGROUND & AIMS: :Two varieties of collagen/sodium hyaluronan membranes were used as dermal substitutes in a biocompatibility implantation study on rats. In order to improve especially the physical and mechanical properties of the material, the membranes were chemically modified using a combination of hexamethylenediisocyanate (HMDC) as a crosslinker and polyoxy-ethylene (POE) as a spacer. According to both macroscopic and microscopic histological observations, the membranes were well accepted by the surrounding host tissue in all the animals. No major differences in relation to the outgrowth of the material by host tissue have been observed between the implant varieties A and B. The most important finding was that no pathological changes or important alterations of the host tissues were detected.
    背景与目标: :在大鼠的生物相容性植入研究中,使用了两种胶原/透明质酸钠膜作为真皮替代物。为了特别提高材料的物理和机械性能,使用六亚甲基二异氰酸酯(HMDC)作为交联剂和聚氧乙烯(POE)作为间隔物的组合对膜进行了化学修饰。根据宏观和微观组织学观察,在所有动物中,膜都被周围宿主组织很好地接受。在植入物品种A和B之间,没有观察到与宿主组织的材料生长有关的主要差异。最重要的发现是未检测到宿主组织的病理变化或重要变化。

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