• 【加拿大安大略省旧门诺派的母乳喂养做法: 一项多种方法研究。】 复制标题 收藏 收藏
    DOI:10.1177/0890334413498305 复制DOI
    作者列表:Norris S,Collin SM,Ingram J
    BACKGROUND & AIMS: BACKGROUND:The Old Order Mennonites (OOM) of rural Ontario have a lifestyle that is very distinct from the rest of Canada. Breastfeeding practices among this community have not been described previously. OBJECTIVES:This study aimed to estimate the prevalence of exclusive breastfeeding (EBF) at 2, 4, and 6 months; to compare the prevalence of EBF among OOM and Canadian women; to investigate factors associated with EBF at 6 months; and to gain qualitative insight into the breastfeeding practices of OOM women. METHODS:Data on maternal characteristics, delivery factors, and infant feeding methods at birth and at 2, 4, and 6 months were obtained from medical records at the Elmira Medical Centre for all births to OOM women between January 2006 and December 2011. Semi-structured interviews were carried out with 2 lactation consultants working at the Elmira Medical Centre. RESULTS:Complete breastfeeding data were available for 195 of 225 OOM women (77.4%). The majority of OOM women initiated breastfeeding (87.9%); 81.4% continued to breastfeed exclusively at 2 months, 74.0% to 4 months, and 36.8% to 6 months. Women who had a homebirth (12.3%) had 2.6-fold higher odds of EBF at 6 months (odds ratio, 2.59; 95% confidence interval, 1.03-6.53) compared with women who delivered in a hospital. Cultural and religious influences and community support were suggested as reasons for the relatively high prevalence of EBF. CONCLUSION:Breastfeeding rates among OOM women are consistently higher in the first 6 months of life compared to the general Canadian population. Homebirth independently predicted increased odds of EBF at 6 months.
    背景与目标:
  • 【在11个国家/地区咨询后,影响妇女选择联合激素避孕方法的因素: CHOICE研究的子分析结果。】 复制标题 收藏 收藏
    DOI:10.3109/13625187.2013.819077 复制DOI
    作者列表:Bitzer J,Cupanik V,Fait T,Gemzell-Danielsson K,Grob P,Oddens BJ,Pawelczyk L,Unzeitig V
    BACKGROUND & AIMS: OBJECTIVES:To investigate which characteristics of women and healthcare professionals (HCPs) were associated with changing to another combined hormonal contraceptive (CHC) method after contraceptive counselling. METHODS:CHOICE was a cross-sectional survey in which 18,787 women were counselled about combined hormonal contraceptives, during which their contraceptive methods preferred both prior to and after counselling were recorded. In this subanalysis, characteristics associated with changing the method after counselling were determined using logistic regression models. RESULTS:The probability of intending to change from the pill to another method was associated with being older; university-educated; being in a steady relationship; a prior unintended pregnancy; a younger HCP or one who recommended methods other than the pill. Changing to the patch was associated with a female HCP or a HCP who recommended the patch or an injectable. Changing to the ring was associated with being over 21 years; university-educated; being in a relationship; previous hormonal method use; and counselling by a female HCP, a HCP < 60 years old, or a HCP who recommended the ring or an implant. The country of residence influenced these changes in a complex pattern. CONCLUSIONS:Women's choice of CHC methods after contraceptive counselling are influenced by their age, educational background, relationship status, prior unplanned pregnancies and country of residence, as well as age, gender and preferences of their HCP.
    背景与目标:
  • 【不同灭活方法对印度口蹄疫病毒疫苗株稳定性的比较】 复制标题 收藏 收藏
    DOI:10.1016/j.biologicals.2017.06.004 复制DOI
    作者列表:Sarkar A,Tamil Selvan RP,Kishore S,Ganesh K,Bhanuprakash V
    BACKGROUND & AIMS: :In this study, the efficiency of binary ethyleneimine (BEI) in combination with formaldehyde (FA) and glutaraldehyde (GTA) in inactivating the Indian FMDV vaccine strains is compared. The acceptable safety of virus inactivation was faster and the inactivation rates were increased many-folds with combination of inactivants than BEI alone. FMDV A was inactivated rapidly than the other two serotypes with BEI + FA combination. Inactivation plots were linear for all the serotypes irrespective of inactivation process. Further, the integrity studies on 146S using serotype specific ELISA indicated no significant change in the antigenic mass of all the serotypes throughout the inactivation process. However, the loss of 146S antigen occurred in the subsequent steps of downstream processing. Further, the studies on intactness of viral RNA using real time PCR indicated the amplification of 1D gene sequences in all the preparations of timed samples irrespective of serotypes/inactivation process. Further, inactivated virus preparation (146S) was more stable at lower temperatures for all the serotypes/inactivation process. Among the combinations of inactivants, BEI + FA out performed compared to BEI + GTA and BEI in terms of inactivation rates, 146S yield and its storage stability, irrespective of the serotypes.
    背景与目标: : 在这项研究中,比较了二元乙烯亚胺 (BEI) 与甲醛 (FA) 和戊二醛 (GTA) 联合灭活印度FMDV疫苗株的效率。与单独使用灭活剂相比,灭活剂的可接受安全性更快,灭活率提高了许多倍。FMDV A比BEI FA组合的其他两种血清型迅速失活。与灭活过程无关,所有血清型的灭活图都是线性的。此外,使用血清型特异性ELISA对146S的完整性研究表明,在整个灭活过程中,所有血清型的抗原质量没有显著变化。然而,146S抗原的损失发生在下游处理的后续步骤中。此外,使用实时PCR对病毒RNA的完整性进行的研究表明,无论血清型/失活过程如何,在所有定时样品的制剂中都扩增了1D基因序列。此外,对于所有血清型/灭活过程,灭活病毒制剂 (146S) 在较低温度下更稳定。在灭活剂的组合中,与BEI GTA和BEI相比,BEI + FA在灭活率,146S产率及其储存稳定性方面进行了比较,而与血清型无关。
  • 【同时使用几种方法估算浮游植物生物量的有用性。】 复制标题 收藏 收藏
    DOI:10.1016/S0932-4739(88)80023-3 复制DOI
    作者列表:Aleya L,Devaux J,Magouri HE,Marvalin O,Amblard C
    BACKGROUND & AIMS: :The annual cycle of phytoplankton biomass was followed in a eutrophic lake (Lake Aydat, Massif Central, France), using classic descriptors (biovolumes and chlorophyll a) as well as adenosine-5'-phosphate (ATP) levels. ATP is the metabolite indicating living biomass. ATP/Cell count and ATP/Chlorophyll a ratios were elevated due to the presence of heterotrophic organisms, such as several species of ciliates. The first ratio is greater than the second. The difference can be explained either by an underestimation of the nanoplankton fraction through the cell count method, or by a lack of sedimentation in the settling chambers used for counting cells.
    背景与目标: : 使用经典的描述符 (生物体积和叶绿素a) 以及腺苷-5 '-磷酸 (ATP) 水平,在富营养化的湖泊 (法国中部Massif中部的Aydat湖) 中跟踪了浮游植物生物量的年度周期。ATP是指示活生物量的代谢产物。由于异养生物 (例如几种纤毛虫) 的存在,ATP/细胞计数和ATP/叶绿素a比率升高。第一个比率大于第二个比率。这种差异可以通过细胞计数方法低估了纳米浮游生物的比例,也可以通过用于计数细胞的沉降室中缺乏沉降来解释。
  • 【甲基丙烯酸甲酯骨水泥制备过程中使用的采样和分析方法的比较。】 复制标题 收藏 收藏
    DOI:10.1080/15459620600732811 复制DOI
    作者列表:Ungers LJ,Vendrely TG
    BACKGROUND & AIMS: :Surgeons, surgical nurses, and other operating room staff are exposed to airborne concentrations of methyl methacrylate during the preparation of orthopedic bone cement. Three sampling and analysis methods have been used to measurement methyl methacrylate in this work environment: (1) direct-reading photoacoustic infrared spectrometry, (2) solid sorbent and gas chromatography with flame ionization detection, and (3) colorimetric detector tubes. Previous studies have measured operating room exposures and judged the efficacy of cement mixing wth little, if any, regard for method sensitivity, detection limits, precision, or accuracy. The present investigation was designed to allow concurrent monitoring of methyl methacrylate levels from the same air volume using each of the three methods. Three popular orthopedic bone cement products were mixed during a number of repeat preparations (n = 36). Airborne concentrations were monitored concurrently during each preparation. Attention was given to the proper treatment of detection limits, and the results are reported both as raw data and descriptive statistics. A one-way ANOVA using a Tukey-Kramer HSD comparison was performed on method-specific results indicating that the photoacoustic infrared spectrometry and solid sorbent, gas chromatography with flame ionization detection are in good agreement, but the colorimetric detector tube method reports significantly different airborne concentrations. It is concluded that previous assessments using the photoacoustic infrared spectrometry and solid sorbent, gas chromatography with flame ionization detection may be relied on, but the detector tube method underreports actual exposures. Accordingly, the results of past exposure assessments and mixing method efficacy studies using colorimetric detector tubes may not be reliable.
    背景与目标: : 外科医生,外科护士和其他手术室工作人员在准备骨科骨水泥期间暴露于空气中浓度的甲基丙烯酸甲酯。在此工作环境中,已使用三种采样和分析方法来测量甲基丙烯酸甲酯 :( 1) 直读光声红外光谱法,(2) 具有火焰离子化检测的固体吸附剂和气相色谱法,以及 (3) 比色检测器管。先前的研究已经测量了手术室的暴露,并判断了水泥混合的功效,如果有的话,则很少考虑方法的灵敏度,检出限,精密度或准确性。本研究旨在允许使用三种方法中的每种方法同时监测同一风量中的甲基丙烯酸甲酯水平。在许多重复准备过程中混合了三种流行的骨科骨水泥产品 (n = 36)。在每次准备过程中同时监测空气中的浓度。注意对检测限的正确处理,并将结果报告为原始数据和描述性统计。使用Tukey-Kramer HSD进行的单因素方差分析比较了方法的特异性结果,表明光声红外光谱法和固体吸附剂,气相色谱和火焰离子化检测法具有良好的一致性,但是比色检测器管法报告的空气浓度明显不同。结论是,可以依靠以前使用光声红外光谱法和固体吸附剂,气相色谱和火焰离子化检测进行的评估,但是检测器管方法低估了实际暴露。因此,使用比色检测器管的过去暴露评估和混合方法功效研究的结果可能不可靠。
  • 【索氏梭菌致死毒素对上皮细胞屏障通透性和细胞间连接的修饰。】 复制标题 收藏 收藏
    DOI:10.1111/j.1462-5822.2006.00687.x 复制DOI
    作者列表:Boehm C,Gibert M,Geny B,Popoff MR,Rodriguez P
    BACKGROUND & AIMS: :Clostridium sordellii lethal toxin (LT) is a glucosyltransferase which inactivates small GTPases from the Rho and Ras families. In the present work, we studied the effects of two variants, LT82 and LT9048, on the integrity of epithelial cell barrier using polarized MCCD (Mouse Cortical Collecting Duct) and MDCK (Madin-Darby Canine Kidney) cells. Our results demonstrate for the first time that LTs have very limited effects on tight junctions. In contrast, we show that both toxins modified the paracellular permeability within 2-4 h. Concomitantly LT82 and LT9048 induced a disorganization of basolateral actin filaments, without modifying apical actin. Both toxins mainly altered adherens junctions by removing E-cadherin-catenin complexes from the membrane to the cytosol. Similar effects on adherens junctions have been observed with other toxins, which directly or indirectly depolymerize actin. Thereby, Rac, a common substrate of both LTs, might play a central role in LT-dependent adherens junction alteration. Here, we show that adherens junction perturbation induced by LTs results neither from a direct effect of toxins on adherens junction proteins nor from an actin-independent Rac pathway, but rather from a Rac-dependent disorganization of basolateral actin cytoskeleton. This further supports that a dynamic equilibrium of cortical actin filaments is essential for functional E-cadherin organization in epithelia.
    背景与目标: : 梭状芽孢杆菌致死毒素 (LT) 是一种葡萄糖基转移酶,可使Rho和Ras家族的小GTPases失活。在目前的工作中,我们使用极化MCCD (小鼠皮质收集管) 和MDCK (Madin-Darby犬肾) 细胞研究了两种变体LT82和LT9048对上皮细胞屏障完整性的影响。我们的结果首次证明了LTs对紧密连接的影响非常有限。相反,我们显示两种毒素在2-4小时内都改变了细胞旁通透性。伴随LT82和LT9048引起基底外侧肌动蛋白丝的混乱,而没有改变顶端肌动蛋白。两种毒素主要通过将E-钙粘蛋白-连环蛋白复合物从膜去除到胞质溶胶来改变粘附连接。其他毒素也观察到对粘附连接的类似作用,这些毒素直接或间接解聚肌动蛋白。因此,Rac是两种LTs的共同底物,可能在依赖LT的粘附结改变中起着核心作用。在这里,我们表明LTs诱导的粘附连接扰动既不是由毒素对粘附连接蛋白的直接作用也不是由肌动蛋白独立的Rac途径引起的,而是由Rac依赖性的基底外侧肌动蛋白细胞骨架的分解引起的。这进一步支持皮质肌动蛋白丝的动态平衡对于上皮细胞中功能性E-钙粘蛋白组织至关重要。
  • 【妇女对计划生育方法行动机制的态度: 西班牙潘普洛纳初级保健中心的调查。】 复制标题 收藏 收藏
    DOI:10.1186/1472-6874-7-10 复制DOI
    作者列表:de Irala J,Lopez del Burgo C,Lopez de Fez CM,Arredondo J,Mikolajczyk RT,Stanford JB
    BACKGROUND & AIMS: BACKGROUND:Informed consent in family planning includes knowledge of mechanism of action. Some methods of family planning occasionally work after fertilization. Knowing about postfertilization effects may be important to some women before choosing a certain family planning method. The objective of this survey is to explore women's attitudes towards postfertilization effects of family planning methods, and beliefs and characteristics possibly associated with those attitudes. METHODS:Cross-sectional survey in a sample of 755 potentially fertile women, aged 18-49, from Primary Care Health Centres in Pamplona, Spain. Participants were given a 30-item, self-administered, anonymous questionnaire about family planning methods and medical and surgical abortion. Logistic regression was used to identify variables associated with women's attitudes towards postfertilization effects. RESULTS:The response rate was 80%. The majority of women were married, held an academic degree and had no children. Forty percent of women would not consider using a method that may work after fertilization but before implantation and 57% would not consider using one that may work after implantation. While 35.3% of the sample would stop using a method if they learned that it sometimes works after fertilization, this percentage increased to 56.3% when referring to a method that sometimes works after implantation. Women who believe that human life begins at fertilization and those who consider it is important to distinguish between natural and induced embryo loss were less likely to consider the use of a method with postfertilization effects. CONCLUSION:Information about potential postfertilization effects of family planning methods may influence women's acceptance and choice of a particular family planning method. Additional studies in other populations are necessary to evaluate whether these beliefs are important to those populations.
    背景与目标:
  • 【使用蛋白质微阵列和非参数方法进行预后的综合方法。】 复制标题 收藏 收藏
    DOI:10.1038/msb4100167 复制DOI
    作者列表:Knickerbocker T,Chen JR,Thadhani R,MacBeath G
    BACKGROUND & AIMS: :Over the past several years, multivariate approaches have been developed that address the problem of disease diagnosis. Here, we report an integrated approach to the problem of prognosis that uses protein microarrays to measure a focused set of molecular markers and non-parametric methods to reveal non-linear relationships among these markers, clinical variables, and patient outcome. As proof-of-concept, we applied our approach to the prediction of early mortality in patients initiating kidney dialysis. We found that molecular markers are not uniformly prognostic, but instead vary in their value depending on a combination of clinical variables. This may explain why reports in this area aiming to identify prognostic markers, without taking into account clinical variables, are either conflicting or show that markers have marginal prognostic value. Just as treatments are now being tailored to specific subsets of patients, our results show that prognosis can also benefit from a 'personalized' approach.
    背景与目标: : 在过去的几年中,已经开发了解决疾病诊断问题的多变量方法。在这里,我们报告了一种针对预后问题的综合方法,该方法使用蛋白质微阵列来测量一组集中的分子标记物,以及非参数方法来揭示这些标记物,临床变量和患者预后之间的非线性关系。作为概念验证,我们将我们的方法应用于开始肾透析的患者的早期死亡率的预测。我们发现,分子标志物的预后并不一致,而是根据临床变量的组合而变化。这可以解释为什么在不考虑临床变量的情况下,旨在识别预后标志物的这一领域的报告存在冲突或表明标志物具有边际预后价值。就像现在针对特定的患者子集进行治疗一样,我们的结果表明,“个性化” 方法也可以使预后受益。
  • 【连续环形撕囊技术的发展,优势和方法。】 复制标题 收藏 收藏
    DOI:10.1016/s0886-3350(13)80870-x 复制DOI
    作者列表:Gimbel HV,Neuhann T
    BACKGROUND & AIMS: :The development of the continuous circular capsulorhexis (CCC) technique has contributed significantly to the safety and effectiveness of cataract extraction and intraocular lens implantation. This technique facilitates every size of smooth, circular, capsular opening, and it produces a strong capsular rim that resists tearing even when stretched during lens material removal or lens implantation. Maintaining the general integrity of the eye and facilitating such procedures as hydrodissection, endolenticular phacoemulsification, capsule polishing, and safe lens implantation in both adults and children are some of the advantages of CCC. This procedure can be performed in several ways, and it has been proven to be consistently reproducible by experienced surgeons.
    背景与目标: : 连续环形撕囊 (CCC) 技术的发展为白内障摘除和人工晶状体植入的安全性和有效性做出了重要贡献。这种技术有利于各种尺寸的光滑,圆形,囊状开口,并且它产生了强大的囊状边缘,即使在去除镜片材料或植入镜片的过程中拉伸,也可以抵抗撕裂。CCC的一些优点是,保持眼睛的整体完整性并促进诸如水解剖,晶状体内超声乳化,胶囊抛光和成人和儿童安全的晶状体植入等程序。此过程可以通过多种方式进行,并且已被经验丰富的外科医生证明是始终如一地可重复的。
  • 【社会行为的分子基础: 模型、方法和进展。】 复制标题 收藏 收藏
    DOI:10.1016/j.conb.2012.08.008 复制DOI
    作者列表:LeBoeuf AC,Benton R,Keller L
    BACKGROUND & AIMS: :Elucidating the molecular and neural basis of complex social behaviors such as communal living, division of labor and warfare requires model organisms that exhibit these multi-faceted behavioral phenotypes. Social insects, such as ants, bees, wasps and termites, are attractive models to address this problem, with rich ecological and ethological foundations. However, their atypical systems of reproduction have hindered application of classical genetic approaches. In this review, we discuss how recent advances in social insect genomics, transcriptomics, and functional manipulations have enhanced our ability to observe and perturb gene expression, physiology and behavior in these species. Such developments begin to provide an integrated view of the molecular and cellular underpinnings of complex social behavior.
    背景与目标: : 阐明复杂社会行为 (例如公共生活,分工和战争) 的分子和神经基础,需要表现出这些多方面的行为表型的模型生物。社会昆虫,如蚂蚁、蜜蜂、黄蜂和白蚁,是解决这一问题的有吸引力的模型,具有丰富的生态和行为学基础。然而,它们的非典型繁殖系统阻碍了经典遗传方法的应用。在这篇综述中,我们讨论了社会昆虫基因组学,转录组学和功能操作的最新进展如何增强了我们观察和干扰这些物种的基因表达,生理和行为的能力。这样的发展开始提供复杂社会行为的分子和细胞基础的综合视图。
  • 【评估用于测量眼底照片上视网膜血管图像宽度的显微和显微密度测量方法。】 复制标题 收藏 收藏
    DOI:10.1007/BF02172974 复制DOI
    作者列表:Delori FC,Fitch KA,Feke GT,Deupree DM,Weiter JJ
    BACKGROUND & AIMS: :The width of retinal vessel images on fundus photographs was determined by projection micrometry and microdensitometry. These methods were evaluated on 12 retinal vessels using the negatives of 570-nm monochromatic fundus photographs. For projection micrometry, the intraobserver reproducibility of vessel width measurements was 1.6%-2.9%, depending upon the experience of the observer. Significant interobserver differences in the measured widths were demonstrated. For microdensitometry, three distinct measurement criteria were used. Significant differences in width as measured by the three criteria were found, but there were no interoperator differences for each criterion. The intraoperator reproducibility of vessel width measurements by microdensitometry was 2.1%-2.5%. Significant differences were found in the vessel widths determined by the micrometry and densitometry methods, and results obtained by micrometry are discussed in terms of edge-detection phenomena.
    背景与目标: : 通过投影显微法和显微密度法确定眼底照片上视网膜血管图像的宽度。使用570 nm单色眼底照片的底片在12条视网膜血管上评估了这些方法。对于投影显微测量,根据观察者的经验,血管宽度测量的观察者内重现性为1.6%-2.9%。证明了观察者之间在测量宽度上的显着差异。对于显微光密度测定法,使用了三种不同的测量标准。通过三个标准测得的宽度存在显着差异,但是每个标准都没有算子间差异。通过显微密度测定法测量血管宽度的操作者内部可重复性为2.1%-2.5%。在通过显微法和光密度法确定的血管宽度中发现了显着差异,并根据边缘检测现象讨论了通过显微法获得的结果。
  • 【雷帕霉素诱导自噬可改善IL-10敲除小鼠的实验性结肠炎并改善肠上皮屏障功能。】 复制标题 收藏 收藏
    DOI:10.1016/j.intimp.2019.105977 复制DOI
    作者列表:Zhao J,Wang H,Yang H,Zhou Y,Tang L
    BACKGROUND & AIMS: BACKGROUND:An impairment of the intestinal barrier function is one of the major characteristics of Crohn's disease (CD). This study aimed to evaluate the impact of autophagy induction by rapamycin on the intestinal epithelial barrier function in CD model mice. METHODS:IL-10 knockout (IL-10 KO) mice were used as the human CD models in this study. All the mice were randomly assigned into four groups, (a) wild-type (WT) group; (b) IL-10 KO group; (c) IL-10 KO + rapamycin group and (d) IL-10 KO + 3-methyladenine (3-MA), containing 6 mice in each group. The disease activity index (DAI), histology, pro-inflammatory cytokines and chemotactic factors in colon tissues, intestinal and colonic permeability, distributions and expressions of tight junction (TJ) proteins, epithelial apoptosis of mice in four groups were evaluated and compared. RESULTS:Autophagy induction by rapamycin treatment ameliorated DAI and histological colitis, decreased pro-inflammatory cytokines (TNF-α, IFN-γ and IL-17) and chemotactic factors (CXCL-1 and CXCL-2), decreased intestinal and colonic permeability, improved the distribution and expression of TJ proteins in IL-10 KO mice. CONCLUSION:Autophagy induction by rapamycin significantly improved intestinal barrier function and protected IL-10 KO mice from the experimental chronic colitis.
    背景与目标:
  • 【法国国家医疗保健系统数据库 (SNDS) 中基于病例的方法鉴定与上消化道出血相关的药物的经验评估。】 复制标题 收藏 收藏
    DOI:10.1002/pds.5038 复制DOI
    作者列表:Thurin NH,Lassalle R,Schuemie M,Pénichon M,Gagne JJ,Rassen JA,Benichou J,Weill A,Blin P,Moore N,Droz-Perroteau C
    BACKGROUND & AIMS: PURPOSE:Upper gastrointestinal bleeding (UGIB) is a severe and frequent drug-related event. In order to enable efficient drug safety alert generation in the French National Healthcare System database (SNDS), we assessed and calibrated empirically case-based designs to identify drug associated with UGIB risk. METHODS:All cases of UGIB were extracted from SNDS (2009-2014) using two definitions. Positive and negative drug controls were used to compare 196 self-controlled case series (SCCS), case-control (CC) and case-population (CP) design variants. Each variant was evaluated in a 1/10th population sample using area under the receiver operating curve (AUC) and mean square error (MSE). Parameters that had major impacts on results were identified through logistic regression. Optimal designs were replicated in the unsampled population. RESULTS:Using a specific UGIB definition, AUCs ranged from 0.64 to 0.80, 0.44 to 0.61 and 0.50 to 0.67, for SCCS, CC and CP, respectively. MSE ranged from 0.07 to 0.39, 0.83 to 1.33 and 1.96 to 4.6, respectively. Univariate regressions showed that high AUCs were achieved with SCCS with multiple drug adjustment and a 30-day risk window starting at exposure. The top-performing SCCS variant in the unsampled population yielded an AUC = 0.84 and MSE = 0.14, with 10/36 negative controls presenting significant estimates. CONCLUSIONS:SCCS adjusting for multiple drugs and using a 30-day risk window has the potential to generate UGIB-related alerts in the SNDS and hypotheses on its potential population impact. Negative control implementation highlighted that low systematic error was generated but that protopathic bias and confounding by indication remained unaddressed issues.
    背景与目标:
  • 【人参皂苷Rk3通过保护结肠屏障和抑制NLRP3炎症小体途径减轻DSS诱导的溃疡性结肠炎。】 复制标题 收藏 收藏
    DOI:10.1016/j.intimp.2020.106645 复制DOI
    作者列表:Tian M,Ma P,Zhang Y,Mi Y,Fan D
    BACKGROUND & AIMS: :Ginsenosides have a variety of pharmacological activities, including immunomodulatory, antitumor and anti-inflammatory activities. However, the effect of Rk3 on ulcerative colitis has rarely been reported. This study evaluated the effect of Rk3 on DSS-induced ulcerative colitis and preliminarily explored the anti-inflammatory mechanisms. Rk3 administration significantly attenuated the weight loss, increased DAI scores, colonic shortening, and increased MPO and iNOS activities caused by DSS in mice. Histological improvement was apparent, tight junctions in the colon were restored, and the levels of short-chain fatty acids (acetic acid, butyric acid and isovaleric acid) were increased. In addition, Rk3 reduced the expression of proinflammatory factors (TNF-α, IL-1β and IL-6), NLRP3, ASC, and Caspase-1, indicating blockade of the NLRP3 inflammasome pathway. These results show that Rk3 can improve DSS-induced ulcerative colitis by protecting intestinal barrier function and inhibiting NLRP3 inflammasome expression, indicating that Rk3 could be used as a potential drug for treating ulcerative colitis.
    背景与目标: : 人参皂苷具有多种药理活性,包括免疫调节,抗肿瘤和抗炎活性。然而,Rk3对溃疡性结肠炎的作用很少报道。本研究评估了Rk3对DSS诱导的溃疡性结肠炎的作用,并初步探讨了其抗炎机制。Rk3给药可显着减轻小鼠的体重减轻,DAI评分增加,结肠缩短以及DSS引起的MPO和iNOS活性增加。组织学改善明显,结肠中的紧密连接恢复,短链脂肪酸 (乙酸,丁酸和异戊酸) 的水平增加。此外,Rk3降低了促炎因子 (TNF-α,IL-1β 和IL-6),NLRP3,ASC和Caspase-1的表达,表明NLRP3炎症小体途径被阻断。这些结果表明,Rk3可以通过保护肠屏障功能和抑制NLRP3炎症小体表达来改善DSS诱导的溃疡性结肠炎,表明Rk3可以作为治疗溃疡性结肠炎的潜在药物。
  • 【渗滤液中难熔有机物的高级氧化-潜在的方法和剩余底物的生物降解性评估。】 复制标题 收藏 收藏
    DOI:10.1080/09593330801985057 复制DOI
    作者列表:Hagman M,Heander E,Jansen JL
    BACKGROUND & AIMS: :An assessment of potential methods suitable for degradation and/or reduction of refractory organics was performed on landfill leachate from SYSAV AB, Malmö, Sweden. Pretreatment of the leachate was performed in a sequence batch reactor designed for nitrification in activated sludge. Oxidation of the leachate was then O3, O3/pH adjustment (pH 9 and 10), H2O2, O3/H2O2 and performic acid in lab-scale reactors. The degradation of organic material was followed with chemical oxygen demand (COD) measurements for all experiments except for the performic acid treatment for which total organic carbon (TOC) measurements were used. The potential degradation of refractory organics into biodegradable organic material was analysed by subsequent oxygen uptake rate (OUR) measurements in laboratory batch reactors. Ozonation of biologically pre-treated leachate increased reduction of the organic material. The most biodegradable organic material was produced after oxidation with only ozone and ozonation at pH 9. Performic acid did not reduce the content of organic material in the leachate. However, a combination of biological pretreatment, chemical oxidation with O3/H2O2 and a subsequent biological process resulted in the most efficient oxidation method for the tested leachate.
    背景与目标: : 对瑞典马尔默SYSAV AB的垃圾填埋场渗滤液进行了适合降解和/或减少难降解有机物的潜在方法的评估。渗滤液的预处理是在设计用于活性污泥硝化的顺序分批反应器中进行的。然后在实验室规模的反应器中,渗滤液的氧化是O3,O3/pH调节 (pH 9和10),H2O2,O3/H2O2和过甲酸。除使用总有机碳 (TOC) 测量的过甲酸处理外,所有实验均对有机材料的降解进行化学需氧量 (COD) 测量。通过随后在实验室间歇反应器中进行的氧气吸收率 (OUR) 测量,分析了难熔有机物可能降解为可生物降解的有机材料。生物预处理渗滤液的臭氧化增加了有机材料的减少。最可生物降解的有机材料是在仅用臭氧和臭氧化在pH 9下氧化后产生的。过甲酸不会降低渗滤液中有机物质的含量。然而,生物预处理,O3/H2O2的化学氧化以及随后的生物过程相结合,可以为测试的渗滤液提供最有效的氧化方法。

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