The alpha subunit of the guanine nucleotide-binding protein Go ("o" for other) is believed to mediate signal transduction between a variety of receptors and effectors. cDNA clones encoding two forms of Go alpha subunit were isolated from a mouse brain library. These two forms, which we call GoA alpha and GoB alpha, appear to be the products of alternative splicing. GoA alpha differs from GoB alpha over the C-terminal third of the deduced protein sequence. Both forms are predicted to be substrates for ADP-ribosylation by pertussis toxin. GoA alpha transcripts are present in a variety of tissues but are most abundant in brain. The GoB alpha transcript is expressed at highest levels in brain and testis. It is possible that GoA alpha and GoB alpha have different functions.
-
【Alternative splicing produces transcripts encoding two forms of the alpha subunit of GTP-binding protein Go.].
【选择性剪接产生转录本，编码两种形式的GTP结合蛋白Go的 α 亚基。】
复制标题
收藏
DOI：10.1073/pnas.87.17.6477文章类型：杂志文章
复制DOI
译文
鸟嘌呤核苷酸结合蛋白Go (“o” 为其他) 的 α 亚基被认为介导多种受体和效应子之间的信号转导。从小鼠脑文库中分离出编码两种形式的Go α 亚基的cDNA克隆。这两种形式，我们称之为GoA alpha和GoB alpha，似乎是替代拼接的产物。在推导的蛋白质序列的C末端三分之一处，GoA α 与GoB α 不同。预计两种形式都是百日咳毒素进行ADP-核糖基化的底物。果阿 α 转录本存在于多种组织中，但在大脑中含量最高。GoB α 转录本在脑和睾丸中以最高水平表达。有可能GoA alpha和GoB alpha具有不同的功能。
