Corynebacterium pseudotuberculosis is the causative agent of several veterinary diseases in a broad range of economically important hosts, which can vary from caseous lymphadenitis in sheep and goats (biovar ovis) to ulcerative lymphangitis in cattle and horses (biovar equi). Existing vaccines against C. pseudotuberculosis are mainly intended for small ruminants and, even in these hosts, they still present remarkable limitations. In this study, we present the complete genome sequence of C. pseudotuberculosis biovar equi strain 258, isolated from a horse with ulcerative lymphangitis. The genome has a total size of 2,314,404 bp and contains 2088 predicted protein-coding regions. Using in silico analysis, eleven pathogenicity islands were detected in the genome sequence of C. pseudotuberculosis 258. The application of a reverse vaccinology strategy identified 49 putative antigenic proteins, which can be used as candidate vaccine targets in future works.
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【Genome sequence of Corynebacterium pseudotuberculosis biovar equi strain 258 and prediction of antigenic targets to improve biotechnological vaccine production.].
【假结核杆菌Biovar equi 258菌株的基因组序列和抗原靶标的预测，以提高生物技术疫苗的生产。】
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DOI：10.1016/j.jbiotec.2012.11.003文章类型：杂志文章
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：假结核杆菌是多种重要经济宿主中几种兽医疾病的病原体，从绵羊和山羊的干酪性淋巴结炎（biovar ovis）到牛和马的溃疡性淋巴管炎（biovar equi）不等。现有的针对假结核杆菌的疫苗主要用于小型反刍动物，即使在这些宿主中，它们仍然存在明显的局限性。在这项研究中，我们介绍了从患有溃疡性淋巴管炎的一匹马中分离出来的假结核梭菌biovar equi菌株258的完整基因组序列。基因组的总大小为2,314,404 bp，包含2088个预测的蛋白质编码区。通过计算机分析，在假结核梭菌258的基因组序列中检测到11个致病岛。反向疫苗学策略的应用确定了49种推定的抗原蛋白，这些蛋白可以用作未来工作中的候选疫苗靶标。
