The antigens in normal human skin defined by antibodies in patients with bullous pemphigoid (BP) were studied by Western immunoblots. Eighteen (90%) of 20 BP sera reacted to a 230-kD antigen. Seven (35%) of the sera reacted to a 160-kD antigen. Two of these reacted only to the 160-kD antigen and five also reacted to the 230-kD antigen. Antibodies to the 160-kD antigen were not present in 25 control sera obtained from normal individuals or patients with other bullous diseases. The 160-kD antigen was present in epidermal extracts of four different specimens of normal human skin but not in dermal extracts or extracts of control cells including melanoma, fibroblasts, lung carcinoma, and colon carcinoma. Monospecific sera with antibodies to either the 230-kD or to the 160-kD antigen reacted solely to their respective target antigens, but not to both, in extracts of epidermis that contained both antigens. The 160-kD antigen broke down to a 140-kD fragment, while the 230-kD antigen was unchanged in the absence of protease inhibitors. Western blot affinity purified antibody to the 160-kD antigen bound only to the basement membrane zone on the epidermal side of 1M NaCl split skin. These results indicate that a 160-kD antigen is a normal component of the basement membrane zone of human skin. The antigen is located on the epidermal side of skin split with 1M NaCl. It is a minor BP antigen, antibodies to which are present in some patients with BP.

译文

:通过Western免疫印迹研究了大疱性天疱疮(BP)患者中抗体定义的正常人皮肤中的抗原。 20个BP血清中有18个(90%)与230 kD抗原反应。七分之三(35%)的血清与160-kD抗原反应。其中两个仅与160-kD抗原反应,另外五个也与230-kD抗原反应。从正常个体或患有其他大疱性疾病的患者获得的25个对照血清中不存在针对160-kD抗原的抗体。 160-kD抗原存在于正常人皮肤的四个不同标本的表皮提取物中,但不存在于包括黑色素瘤,成纤维细胞,肺癌和结肠癌在内的真皮提取物或对照细胞提取物中。在包含两种抗原的表皮提取物中,具有针对230-kD或160-kD抗原的抗体的单特异性血清仅与它们各自的靶抗原反应,而对两种抗原均不反应。 160 kD抗原分解为140 kD片段,而230 kD抗原在不存在蛋白酶抑制剂的情况下保持不变。 Western blot亲和纯化的针对160-kD抗原的抗体仅与1M NaCl分裂皮肤的表皮侧的基膜区结合。这些结果表明160-kD抗原是人皮肤基底膜区的正常成分。抗原位于用1M NaCl分裂的皮肤的表皮侧。它是次要的BP抗原,某些BP患者中存在针对该抗体的抗体。

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