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首页 > 医学词汇大全 > Patient-Derived tumor Xenograft
Patient-Derived tumor Xenograft

肿瘤

关键词肿瘤 实验材料 移植模型

词汇介绍

拓展阅读

解析

Patient   英  ['peɪʃ(ə)nt]   美 [ˈpeʃənt]

释    义   adj. 有耐性的,能容忍的

               n. 病人;患者

同根词   adv.patiently 耐心地;有毅力地

例    句   She watched the patient all night.  她整夜看护那位病人。

 

Derived   英  [dɪ'raɪvd]   美  [dɪ'raɪvd]

释    义   adj. 导出的;衍生的,派生的

               v. 得到;推断(derive的过去分词);由…而来

同根词   adj.derivative 派生的;引出的 derivational 得来的;诱导的

例    句   All they know and deal with is derived from experience.  它们所知道的和所处理的,都是从经验导出来的。

 

tumor   英  ['tju:mə(r)]   美  ['tjʊmɚ]

释    义   n. 肿瘤;肿块;赘生物

例    句   Tumor growth in the body though is a local.  肿瘤虽然是生长在身体的某一局部。

 

Xenograft   美  ['zenəɡrɑ:ft, 'zi:-, -ɡræft]

释    义   n. [医] 异种移植;[医] 异种移植物

同根词   adj.xenogeneic 异种的;异基因的

例    句   The role of spleen in rejection of concordant liver xenograft.  异种肝移植免疫排斥反应中脾脏的作用。

概述

人源肿瘤异种移植模型,简称为PDX模型,是一种将肿瘤患者的肿瘤组织移植到重症免疫缺陷小鼠(NSG)体内,并使肿瘤组织在小鼠体内生长而构建成的一种肿瘤模型。因为该模型相较于其他动物模型,能够更好的模拟人肿瘤组织生长情况及肿瘤环境,因此该模型被广泛应用于肿瘤基础研究以及肿瘤药物临床前药效评估等。原理PDX模型是将通过手术从肿瘤患者体内获得的新鲜肿瘤组织经过一定处理后移植到重症免疫缺陷小鼠(NSG)体内,依靠小鼠提供的微环境进行生长,其特点和价值不同于人源性肿瘤细胞系异种移植模型。PDX模型保持了

Generation of stable PDX derived cell lines using conditional reprogramming复制标题

使用条件重编程生成稳定的PDX衍生细胞系

发表时间:2017-12-06

影响因子:10.7

作者: Alexandra Borodovsky

期刊:Molecular Cancer

Recently, Conditional Reprogramming (CR) technology has been successfully used to achieve sustained expansion of human normal and tumor epithelial cells. This method is based on co-culture of epithelial cells with growth-arrested mouse 3 T3-J2 fibroblast feeders in the presence of a Rho kinase inhibitor. Epithelial cells grown under CR conditions maintain genomic stability and exhibit many properties of adult basal stem cells, but do not express markers of pluripotency such as Oct4 or Sox2 [3]. Recently, these methods have also been used to expand and characterize cancer stem cells. Importantly, cell proliferation by the CR method is reversible, with removal from the growth-stimulating conditions generally leading to differentiation of these stem-like cells to their committed fate. Primary tumor cells expanded in CR cultures have been shown to maintain the characteristics of the parental tumor sample, thereby enabling in vitro chemosensitivity screening assays for personalized drug profiling. Here we describe a method for using CR technology to generate and expand stable PDX cell lines for in vitro and in vivo applications. The PDX tumors were enzymatically disassociated, depleted of murine cells, and CR cultures were established and expanded into stable CR-PDX lines for use in preclinical applications.

译文

最近,条件重编程(CR)技术已成功用于实现人正常和肿瘤上皮细胞的持续扩增。该方法基于在Rho激酶抑制剂存在下上皮细胞与生长停滞的小鼠3 T3-J2成纤维细胞饲养细胞的共培养。在CR条件下生长的上皮细胞维持基因组稳定性并表现出成体基底干细胞的许多特性,但不表达多能性的标记,例如Oct4或Sox2 [3]。最近,这些方法也被用于扩展和表征癌症干细胞。重要的是,通过CR方法的细胞增殖是可逆的,从生长刺激条件中去除通常导致这些干细胞样细胞分化成其定型的命运。已显示在CR培养物中扩增的原发性肿瘤细胞维持亲代肿瘤样品的特征,从而能够进行用于个性化药物分析的体外化学敏感性筛选测定。在这里,我们描述了使用CR技术生成和扩展稳定的PDX细胞系的方法,用于体外和体内应用。将PDX肿瘤酶促解离,耗尽鼠细胞,建立CR培养物并扩增成稳定的CR-PDX系用于临床前应用。