Nicotinamide 英 /,nɪkə'tɪnəmaɪd/ 美 /,nɪkə'tɪnəmɪd/
释 义 n. [生化] 烟酰胺；烟碱；尼克酰胺
例 句 He used nicotinamide to tranquilize the animal. 他用烟酰胺使动物镇静。
释 义 n. [生化] 甲基转移酶
例 句 DNA production independent of its methyltransferase activity. DNA生产独立于其甲基转移酶活性。
作者： Kamalakannan Palanichamy
期刊：Clin. Cancer Res.
NNMT activity in the GBM and isogenic cell lines was determined by measuring the concentration of nicotinamide (NA) and methyl nicotinamide (MNA) using LC-MS/MS in the multiple reactions monitoring (MRM) mode. The NNMT expression levels in the cell lines tested corresponded with the concentration of MNA in each cell line. The proliferative potential of NNMT isogenic cell lines was determined using MTT assay. KD cells demonstrated a decreased proliferative potential compared to the WT and OE cell lines. After irradiating the isogenic cell lines, KD cells showed an increased growth inhibition and decreased colony forming ability compared to the other isogenic cell lines. Of note, adding temozolomide to radiation treatment did not significantly increase the growth inhibition of KD cells. Interestingly, western blot analysis demonstrated that the KD cell line expresses a basal level of the apoptotic marker cleaved caspase 3. Overall, this suggests that inhibition of NNMT alone may decrease GBM viability, perhaps as a result of the deactivated pro-survival pathway.