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extracellular vesicles

肿瘤

关键词肿瘤 临床研究术语 EVs、液体活检

词汇介绍

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解析

Extracellular   英 /ekstrə'seljʊlə/   美 /,ɛkstrə'sɛljʊlɚ/

释    义   adj. (位于或发生于)[生物] 细胞外的(副词extracellularly)

例    句   At the bottom right, orally-active compounds against more conventional extracellular targets is a tricky one. 右下角,口腔活性化合物对抗传统的细胞外靶点是一个棘手的问题。

 

Vesicles 

释    义   n. 囊泡;小水泡(vesicle的复数)

例    句   Zhu had a simpler idea: He dumped a high concentration of micelles-small balls of huddled fatty acids- into a suspension of vesicles. 朱先生有个更简便的主意:将高浓度的微胞倾入囊泡悬浮液中,那些微胞是由脂肪酸凝集成的小球。

概述

细胞外囊泡(EVs)是指从细胞膜上脱落或者由细胞分泌的双层膜结构的囊泡状小体,直径从40 nm到1000 nm不等。胞外囊泡主要由微囊泡(Microvesicles,  MVs)和外泌体(Exosomes, Exs)组成,微囊泡是细胞激活、损伤或凋亡后从细胞膜脱落的小囊泡,直径约为100 nm-1000 nm。EVs最初仅被视作细胞的“垃圾袋”,用于清除不必要的大分子,但现在其被认为是细胞间信号的运载体,可用于细胞间通讯。EVs表面蛋白信号分

Glycan modification of glioblastoma-derived extracellular vesicles enhances receptor-mediated targeting of dendritic cells复制标题

胶质母细胞瘤来源的细胞外囊泡的聚糖修饰增强受体介导的树突状细胞靶向

发表时间:2019-07-23

影响因子:11.0

作者: Sophie A. Dusoswa

期刊:J Extracell Vesicles

Glioblastoma is the most prevalent and aggressive primary brain tumour for which total tumour lysate-pulsed dendritic cell vaccination is currently under clinical evaluation. Glioblastoma extracellular vesicles (EVs) may represent an enriched cell-free source of tumour-associated (neo-) antigens to pulse dendritic cells (DCs) for the initiation of an anti-tumour immune response. Capture and uptake of EVs by DCs could occur in a receptor-mediated and presumably glycan-dependent way, yet the glycan composition of glioblastoma EVs is unknown. Here, we set out to characterize the glycocalyx composition of glioblastoma EVs by lectin-binding ELISA and comprehensive immunogold transmission electron microscopy (immuno-TEM). The surface glycan profile of human glioblastoma cell line-derived EVs (50-200 nm) was dominated by α-2,3- and α-2,6 linked sialic acid-capped complex N-glycans and bi-antennary N-glycans. Since sialic acids can trigger immune inhibitory sialic acid-binding Ig-like lectin (Siglec) receptors, we screened for Siglec ligands on the EVs. Glioblastoma EVs showed significant binding to Siglec-9, which is highly expressed on DCs. Surprisingly, however, glioblastoma EVs lack glycans that could bind Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin (DC-SIGN, CD209), a receptor that mediates uptake and induction of CD4+ and CD8+ T cell activation.

译文

胶质母细胞瘤是最普遍和最具侵略性的原发性脑部肿瘤,目前正在临床评估总的肿瘤裂解物脉冲树突状细胞疫苗接种。胶质母细胞瘤细胞外囊泡(EVs)可能代表脉冲树突细胞(DC)的肿瘤相关(neo-)抗原的丰富的无细胞来源,从而引发了抗肿瘤免疫反应。 DC可以通过受体介导的和大概是聚糖依赖性的方式捕获和吸收EV,但胶质母细胞瘤EV的聚糖组成尚不清楚。在这里,我们着手通过凝集素结合ELISA和全面的免疫金透射电镜(immuno-TEM)表征胶质母细胞瘤电动汽车的糖萼成分。人成胶质细胞瘤细胞系衍生的电动汽车(50-200 nm)的表面聚糖谱主要由α-2,3-和α-2,6连接的唾液酸封端的复合N-聚糖和双触角N-聚糖主导。由于唾液酸可以触发免疫抑制性唾液酸结合Ig样凝集素(Siglec)受体,我们筛选了电动汽车上的Siglec配体。胶质母细胞瘤电动汽车与Siglec-9具有显着结合,而Siglec-9在DC上高度表达。然而,令人惊讶的是,成胶质细胞瘤电动车缺乏能够结合树突状细胞特异性细胞间粘附分子-3-抓取非整联蛋白(DC-SIGN,CD209)的聚糖,该受体介导摄取和诱导CD4 +和CD8 + T细胞活化。