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首页 > 医学词汇大全 > Merkel cell carcinoma
Merkel cell carcinoma

肿瘤

关键词肿瘤 疾病 恶性肿瘤

词汇介绍

拓展阅读

解析

Merkel 

       n. 默克尔(姓氏)

       "If at all, this discussion belongs at the end so I don't find it particularly fitting that we are now once again conducting it in the middle of the crisis, as if it were the answer, " Merkel said. “如果欧元债券是解决办法的话,这场讨论(指是否发行欧元债券)也应该在危机最后阶段无路可走时才进行,所以我认为在危机中间再次进行这个议题不太合适,”默克尔说。

 

Cell   英 /sel/   美 /sɛl/

       n. 细胞;电池;蜂房的巢室;单人小室;vi. 住在牢房或小室中

同根词   cellularity n. 细胞性;多孔性;细胞结构

       One of its purposes is to register for all notifications which occur on that cell, node, or server. 其目的之一就是为该单元、节点或服务器上发生的所有通知进行注册。

 

Carcinoma   英 /,kɑːsɪ'nəʊmə/   美 /,kɑrsɪ'nomə/

       n. [肿瘤] 癌;复数 carcinomas或carcinomata

       You may see 'undifferentiated carcinoma of the oesophagus' in your medical reports if you have this type of oesophageal cancer. 如果你患有这种类型的食管癌,你可能会在你的医学报告中看到“食管癌的未分化”。

概述

默克尔细胞癌(MCC)是原发于皮肤的一种侵袭性很高的恶性肿瘤,多数认为由表皮Markel细胞发生。1972年Toker首先描述也称之为小梁状肿瘤,非常罕见,在美国每年新诊断的病例大约为2488例,然而近几年来发病率逐年升高。它的发病率仅为黑色素瘤的三十五分之一,但致死率却是黑色素瘤的3倍多。因MMC肿瘤细胞质内有神经内分泌颗粒出现,也被称作原发于皮肤的神经内分泌癌,主要发生于老年人的头颈部及四肢,具有独特的超微结构改变和免疫组化染色特征。手术切除后,易局部复发,也可远处转移,在皮肤源性的肿瘤

CRISPR/Cas9 Editing of the Polyomavirus Tumor Antigens Inhibits Merkel Cell Carcinoma Growth In Vitro复制标题

CRISPR/Cas9多瘤病毒肿瘤抗原的编辑抑制Merkel细胞癌的体外生长

发表时间:2019-08-28

影响因子:6.2

作者: Arturo Temblador

期刊:Cancers

Merkel cell carcinoma (MCC) is an aggressive type of skin cancer whose main causative agent is Merkel cell polyomavirus (MCPyV). MCPyV is integrated into the genome of the tumor cells in most MCCs. Virus-positive tumor cells constitutively express two viral oncoproteins that promote cell growth: the small (sT) and the large (LT) tumor antigens (TAs). Despite the success of immunotherapies in patients with MCC, not all individuals respond to these treatments. Therefore, new therapeutic options continue to be investigated. Herein, we used CRISPR/Cas9 to target the viral oncogenes in two virus-positive MCC cell lines: MS-1 and WAGA. Frameshift mutations introduced in the target sequence upon repair of the Cas9-induced DNA break resulted in decreased LT protein levels, which subsequently impaired cell proliferation, caused cell cycle arrest, and led to increased apoptosis. Importantly, a virus-negative non-MCC cell line (HEK293T) remained unaffected, as well as those cells expressing a non-targeting single-guide RNA (sgRNA). Thus, we presumed that the noted effects were not due to the off-target activity of the TAs-targeting sgRNAs. Additionally, WAGA cells had altered levels of cellular proteins involved in cell cycle regulation, supporting the observed cell cycle. Taken together, our findings provide evidence for the development of a CRISPR/Cas9-based therapeutic option for virus-positive MCC.

译文

Merkel细胞癌(MCC)是一种侵袭性皮肤癌,其主要致病因子是Merkel细胞多瘤病毒(MCPyV)。 MCPyV被整合到大多数MCC中的肿瘤细胞的基因组中。病毒阳性肿瘤细胞组成型表达两种促进细胞生长的病毒癌蛋白:小(sT)和大(LT)肿瘤抗原(TA)。尽管MCC患者的免疫疗法取得了成功,但并非所有人都对这些治疗有反应。因此,继续研究新的治疗选择。在本文中,我们使用CRISPR / Cas9靶向两种病毒阳性MCC细胞系中的病毒癌基因:MS-1和WAGA。在修复Cas9诱导的DNA断裂时在靶序列中引入的移码突变导致LT蛋白水平降低,其随后损害细胞增殖,引起细胞周期停滞,并导致细胞凋亡增加。重要的是,病毒阴性的非MCC细胞系(HEK293T)以及表达非靶向单指导RNA(sgRNA)的那些细胞保持不受影响。因此,我们推测所述效果不是由于靶向靶向sgRNA的脱靶活性。另外,WAGA细胞具有改变的参与细胞周期调节的细胞蛋白水平,支持观察到的细胞周期。总之,我们的研究结果为开发基于CRISPR / Cas9的病毒阳性MCC治疗选择提供了证据。