首页 > 肿瘤医学词汇大全 > Programmed cell Death protein 1
Intestinal IFN-γ-producing type 1 regulatory T cells coexpress CCR5 and programmed cell death protein 1 and downregulate IL-10 in the inflamed guts of patients with inflammatory bowel disease.
肠道产生 IFN-γ 的 1 型调节性 T 细胞协同表达 CCR5 和程序性细胞死亡蛋白 1,下调炎症性肠病患者炎症肠中的 IL-10。
IL-10 IL-23 Inflammatory bowel disease regulatory T cells
下载

摘要

BACKGROUND:IL-10 is an anti-inflammatory cytokine required for intestinal immune homeostasis. It mediates suppression of T-cell responses by type 1 regulatory T (TR1) cells but is also produced by CD25+ regulatory T (Treg) cells.
OBJECTIVE:We aimed to identify and characterize human intestinal TR1 cells and to investigate whether they are a relevant cellular source of IL-10 in patients with inflammatory bowel diseases (IBDs).
METHODS:CD4+ T cells isolated from the intestinal lamina propria of human subjects and mice were analyzed for phenotype, cytokine production, and suppressive capacities. Intracellular IL-10 expression by CD4+ T-cell subsets in the inflamed guts of patients with IBD (Crohn disease or ulcerative colitis) was compared with that in cells from noninflamed control subjects. Finally, the effects of proinflammatory cytokines on T-cell IL-10 expression were analyzed, and IL-1β and IL-23 responsiveness was assessed.
RESULTS:Intestinal TR1 cells could be identified by coexpression of CCR5 and programmed cell death protein 1 (PD-1) in human subjects and mice. CCR5+PD-1+ TR1 cells expressed IFN-γ and efficiently suppressed T-cell proliferation and transfer colitis. Intestinal IFN-γ+ TR1 cells, but not IL-7 receptor-positive TH cells or CD25+ Treg cells, showed lower IL-10 expression in patients with IBDs. TR1 cells were responsive to IL-23, and IFN-γ+ TR1 cells downregulated IL-10 with IL-1β and IL-23. Conversely, CD25+ Treg cells expressed higher levels of IL-1 receptor but showed stable IL-10 expression.
CONCLUSIONS:We provide the first ex vivo characterization of human intestinal TR1 cells. Selective downregulation of IL-10 by IFN-γ+ TR1 cells in response to proinflammatory cytokines is likely to drive excessive intestinal inflammation in patients with IBDs.

译文

背景: IL-10 是肠道免疫稳态所必需的抗炎细胞因子。它介导 1 型调节性 T (TR1) 细胞对 T 细胞反应的抑制,但也由调节性 T (Treg) 细胞产生。
目的: 我们旨在鉴定和描述人肠道 TR1 细胞,并研究它们是否是炎症性肠病 (IBDs) 患者 IL-10 的相关细胞来源。
方法: 从人类受试者和小鼠的肠道固有层中分离出的 CD4 T 细胞进行表型、细胞因子产生和抑制能力分析。将 IBD (克罗恩病或溃疡性结肠炎) 患者炎症内脏中 CD4 T 细胞亚群的细胞内 IL-10 表达与非炎症对照组的细胞进行比较。最后,分析促炎细胞因子对 T 细胞 IL-10 表达的影响,并评估 IL-1 β 和 IL-23 反应性。
结果: 通过 CCR5 和程序性细胞死亡蛋白 1 (PD-1) 在人体和小鼠中的共表达,可以鉴定出肠 TR1 细胞。CCR5 PD-1 TR1 细胞表达 IFN-γ,并有效抑制 T 细胞增殖和转移结肠炎。肠道 IFN-γ TR1 细胞,而非 IL-7 受体阳性的 TH 细胞或 cd4 + 调节性 t 细胞,在 IBDs 患者中显示出较低的 IL-10 表达。TR1 细胞对 IL-23 有反应,IFN-γ TR1 细胞与 IL-10 β 和 IL-1 IL-23 下调。相反,cd4 + 调节性 t 细胞表达更高水平的 IL-1 受体,但表现出稳定的 IL-10 表达。
结论: 我们首次提供了人肠道 TR1 细胞的体外表征。IFN-γ TR1 细胞对促炎细胞因子的反应选择性下调 IL-10 可能导致 IBDs 患者肠道过度炎症。

Programmed cell Death protein 1

肿瘤 跨膜蛋白 临床研究术语
概述  :  

程序性死亡蛋白1,也称作PD-1和CD279(分化簇279,cluster of differentiation 279),是一种由288个氨基酸组成的I型跨膜蛋白,属于B7-CD28受体超家族成员,其编码基因为PDCD1,通过T细胞抗原受体和细胞因子受体在T细胞上被诱导表达。是抗原活化的T细胞上的抑制性受体,在诱导和维持对自身的免疫耐受中起关键作用。现研究证明较多类型肿瘤细胞中均存在高表达PD-1的配体PD-L1,从而诱导患者体内T细胞凋亡,使得肿瘤细胞能够免疫逃逸;靶向PD-1或者PD-

Programmed   /'proɡrəmd/

       adj. 程序化的,程控的;v. 规划;制作节目(program的过去分词)

       Are they programmed for success or failure?  他们的人生规划是成功,还是失败?  

 

Death   /dɛθ/ 

       死神、死亡、死

       How did you learn about his death ?  你怎么知道关于他的死亡原因?

 

Protein   英 /'prəʊtiːn/   美 /'protin/ 

       n. 蛋白质;朊;adj. 蛋白质的

       Where do you get your protein?  你从哪里获得蛋白质?

请扫描右侧二维码,免费查看词汇专业知识背景