摘要

This is the first description of the detection of pancreatic adenocarcinoma peritoneal metastasis by established radiolabeled polymerase chain reaction (PCR) based Ki-ras mutational analysis. The present study evaluates both routine cytology and Ki-ras mutational analysis in the detection of peritoneal micrometastases in 24 subjects with pancreatic adenocarcinoma compared to seven control cases of chronic pancreatitis and seven control cases of cholecystitis. Locoregional extension, vascular invasion, and distal metastases were confirmed in 21/24 (88%) of the subjects with pancreatic adenocarcinoma by compute tomography, angiography, endosonography, or laparoscopy. The most common site of histologically confirmed extrapancreatic involvement was the vasculature (29%), followed by the liver (25%), duodenum (17%), peritoneum (17%), and lymph nodes (12%). Peritoneal lavage cytology was positive in 3/24 (12%) cases of pancreatic carcinoma while Ki-ras codon 12 mutational analysis was positive in 2/24 (8%). Two histologically confirmed cases of peritoneal metastases were not detected by either methodology, while peritoneal lavage cytology detected malignant cells in one case with histologically confirmed lymph node metastasis.

译文

这是通过建立基于放射性标记的聚合酶链反应 (PCR) 的 Ki-ras 突变分析检测胰腺癌腹膜转移的第一个描述。本研究评估了在 24 例胰腺癌受试者中检测腹膜微转移的常规细胞学和 Ki-ras 突变分析,并与 7 例慢性胰腺炎对照病例和 7 例胆囊炎对照病例进行了比较。21/24 (88%) 的胰腺癌患者通过计算机断层扫描、血管造影、腔内血管造影或腹腔镜检查证实了局部延伸、血管侵犯和远端转移。组织学确认的胰腺外受累最常见的部位是脉管系统 (29%),其次是肝脏 (25%) 、十二指肠 (17%) 、腹膜 (17%), 和淋巴结 (12%)。3/24 例 (12%) 胰腺癌腹腔灌洗细胞检测呈阳性,而 Ki-ras 密码子 12 突变分析呈阳性,占 2/24 (8%)。两例经组织学证实的腹膜转移病例均未通过两种方法检测到,而腹腔灌洗细胞学检查在 1 例经组织学证实的淋巴结转移病例中检测到恶性细胞。

exfoliative cytology

肿瘤 临床检验 诊断方式
概述  :  

脱落细胞学是采集人体各部位,特别是管腔器官表面的脱落细胞,经过染色后用显微镜观察这些细胞并作出诊断的临床检验学科。又叫诊断细胞学或临床细胞学,是一门在组织病理基础上发展起来的新兴学科故又称脱落细胞病理学。标本来源有自然排出物、体腔抽出液和细针穿刺吸取液。 脱落细胞学检查原理脱落细胞学检查的原理是基于肿瘤细胞和正常细胞的形态有差异。比如肿瘤脱落细胞的一般特征是(1)核异型性:核大小不等、核染色质深染粗糙、核畸形、核浆明显增大、核仁增大增多、核分裂增多,病理性核分裂像、瘤巨细胞、裸核。

Exfoliative 

释    义   adj. 表皮剥脱的;鳞片样脱皮的

例    句   To observe the effect of heparin sodium cream(HEPUDIOD) combined with corticosteroid cream in treating exfoliative cheilitis. 观察肝素钠乳膏(海普林)联合皮质类固醇软膏治疗剥脱性唇炎的效果。

 

Cytology   英 /saɪˈtɒlədʒi/   美 /saɪˈtɑːlədʒi/

释    义   n. 细胞学

同根词   cytoplasmic adj. 细胞质的

               cytogenetic adj. 细胞生成的;细胞发生的

               cytogenetical adj. 细胞遗传学的

               cytologic adj. 细胞学的

               cytological adj细胞学的

               cytolytic adj. 溶细胞的,细胞溶解的

               cytopathogenic adj. 致细胞病变的

               cytoplastic adj. 胞体质的;细胞质的(等于cytoplasm)

               cytoplasm n. [细胞] 细胞质;

例    句   There are obvious difference on morphology and cytology between polypoid and diploid . 四倍体与二倍体在形态学和细胞学上有明显差异。

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