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Nude Mice

肿瘤

关键词肿瘤 实验动物 突变小鼠

词汇介绍

拓展阅读

解析

Nude   英 /njuːd/   美 /nuːd/

释    义   adj. 裸的,裸体的;无装饰的;与生俱有的;n. 裸体;裸体画

例    句   The relevance of using nude mouse models for evaluating drug delivery to human tumors has recently been questioned by numerous researchers. 使用裸鼠模型评估药物给药对人类肿瘤的相关性最近受到许多研究人员的质疑。

 

Mice   英 /maɪs/   美 /maɪs/

释    义   n. 老鼠(mouse的复数)

例    句   He has gathered evidence from mice to support this idea, but whether it is the case in people has yet to be tested. 他已经从老鼠实验中收集了支持这一观点的证据,但它对人是否也起作用还有待进一步测试。

概述

裸鼠,即是先天性胸腺缺陷的突变小鼠,是由于第Ⅶ连锁群(Linkage group)内裸体位点的等位基因发生纯合而形成的突变小鼠新品种。裸鼠是随着近代医学发展,特别是肿瘤学和免疫学研究的需要,在近十几年中发展起来的动物模型。在肿瘤学、免疫学、药品与生物制品的安全性评价及有效药品的筛选等实验方面有着特殊的价值。裸鼠(纯合子nu/nu突变鼠)主要表现为无毛(但可看到一种细毛组织学证明有被毛滤泡)以及缺乏正常胸腺。 杂合子小鼠(nn/+)各方面表现都正常。小鼠中有若干突变基因,它可产生一种

Synergistic Antitumorigenic Activity of Calcitriol with Curcumin or Resveratrol is Mediated by Angiogenesis Inhibition in Triple Negative Breast Cancer Xenografts复制标题

三阴性乳腺癌移植瘤的血管生成抑制介导了骨化三醇与姜黄素或白藜芦醇的协同抗肿瘤活性。

发表时间:2019-11-06

影响因子:6.2

作者: Janice García-Quiroz

期刊:Cancers (Basel)

Calcitriol is a multitarget anticancer hormone; however, its effects on angiogenesis remain contradictory. Herein, we tested whether the antiangiogenic phytochemicals curcumin or resveratrol improved calcitriol antitumorigenic effects in vivo. Triple-negative breast cancer tumoral cells (MBCDF-T) were xenografted in nude mice, maintaining treatments for 3 weeks.  Tumor onset, volume and microvessel density were significantly reduced in mice coadministered with calcitriol and curcumin (Cal+Cur). Vessel count was also reduced in mice simultaneously treated with calcitriol and resveratrol (Cal+Rsv). Cal+Cur and Cal+Rsv treatments resulted in less tumor activated endothelium, as demonstrated by decreased tumor uptake of integrin-targeted biosensors in vivo. The renal gene expression of Cyp24a1 and Cyp27b1 suggested increased calcitriol bioactivity in the combined regimens. In vitro, the phytochemicals inhibited both MBCDF-T and endothelial cells proliferation, while potentiated calcitriol's ability to reduce MBCDF-T cell-growth and endothelial cells migration. Resveratrol induced endothelial cell death, as deduced by increased sub-G1 cells accumulation, explaining the reduced tumor vessel number in resveratrol-treated mice, which further diminished when combined with calcitriol. In conclusion, the concomitant administration of calcitriol with curcumin or resveratrol synergistically promoted anticancer effects in vitro and in vivo in human mammary tumor cells.

译文

骨化三醇是一种多靶点的抗癌激素,但其对血管生成的影响仍然存在矛盾。在此,我们在体内测试了抗血管生成的植物化学物质姜黄素或白藜芦醇是否改善了骨化三醇的抗肿瘤作用。将三阴性乳腺癌肿瘤细胞(MBCDF-T)移植于裸鼠体内,维持治疗3周。与钙三醇和姜黄素(Cal+Cur)合用可显著降低小鼠肿瘤的发生率、体积和微血管密度。同时给予钙三醇和白藜芦醇(Cal+Rsv)的小鼠血管计数也降低。Cal+Cur和Cal+Rsv治疗导致肿瘤激活的内皮细胞减少,整合素靶向生物传感器在体内的肿瘤摄取减少就证明了这一点。肾组织Cyp24a1和Cyp27b1基因表达提示联合用药可提高骨化三醇的生物活性。在体外,这些植物化学物质抑制了MBCDF-T和内皮细胞的增殖,同时增强了骨化三醇抑制MBCDF-T细胞生长和内皮细胞迁移的能力。白藜芦醇通过增加亚G1细胞的积累而导致内皮细胞死亡,这解释了白藜芦醇治疗的小鼠肿瘤血管数量减少,与骨化三醇联合治疗后肿瘤血管数量进一步减少。总之,钙三醇与姜黄素或白藜芦醇合用可协同促进乳腺癌细胞的体内外抗肿瘤作用。