摘要

There is increasing interest in the development of rapid assays of radiosensitivity which can be used on clinical specimens. Unfortunately, the measurement of radiation survival using clonogenic assays, which are the established standard, can be difficult and time consuming. We have used the MTT assay to measure the radiation survival of four lymphoblastoid cell lines with low plating efficiencies. We measured surviving fractions both when the irradiated cells had regained exponential growth and when the non-irradiated cells had undergone four or more doublings. The results were compared to surviving fractions measured by clonogenic assay. We found both methods could be used successfully to rank the cell lines in order of radiosensitivity. However, cells exposed to the higher radiation doses in the MTT assay did not always regain exponential growth, limiting the dose range for which the assay was useful. We also found the best correlation between the two assays was sometimes obtained by using the MTT surviving fractions from different days for different radiation dose levels. Thus, although the MTT assay can be used to measure radiation survival in relation to other cell lines, its use can be complicated by restrictions on radiation dose ranges and difficulties with data interpretation.

译文

人们对可用于临床标本的放射敏感性快速检测方法的发展越来越感兴趣。不幸的是,使用克隆基因分析来测量辐射存活率,这是既定的标准,可能既困难又耗时。我们使用 MTT 法测量了四种低电镀效率的淋巴母细胞系的辐射存活。我们测量了照射后细胞恢复指数增长和未照射细胞经历四次或更多次重复时的存活分数。将结果与克隆形成试验测得的存活分数进行比较。我们发现两种方法都可以成功地按照放射敏感性对细胞系进行排序。然而,在 MTT 试验中暴露于较高辐射剂量的细胞并不总是恢复指数增长,限制了试验有用的剂量范围。我们还发现,对于不同的辐射剂量水平,这两种试验之间的最佳相关性有时是通过使用不同天数的 MTT 存活部分获得的。因此,尽管 MTT 法可以用于测量与其他细胞系相比的辐射存活率,但其使用可能会因辐射剂量范围的限制和数据解释的困难而变得复杂。

MTT assay

肾内泌尿 细胞活性测定 实验技术
概述  :  

MTT是一种粉末状化学试剂,全称为3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di- phenytetrazoliumromide,汉语化学名为 3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐,商品名:噻唑蓝。是一种黄颜色的染料,CAS No. : 298-93-1。MTT 法是Mosmann 1983年报道的,以后此方法得到了迅速发展并广泛应用于临床与科研研究。MTT检测法是一种检测细胞存活和生长的方法,该检测法主要有两

MTT

释    义   n. 3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐

例    句   MTT reduction occurs throughout a cell and can be significantly affected by a number of factors, including metabolic and energy perturbations, changes in the activity of oxidoreductases, endo-/exocytosis and intracellular trafficking. MTT的减少发生在整个细胞中,可以受到许多因素的显著影响,包括代谢和能量的扰动、氧化还原酶活性的变化、胞内外作用和细胞内运输。

 

Assay   英 /əˈseɪ/   美 /əˈseɪ/

释    义   n. 化验;试验

               vt. 分析;化验;尝试

               vi. 鉴定;经检验证明内含成分

同根词   assayer n. 试金者,尝试者;分析专家

例    句   The next step is to use these antibodies in a sensitive screening assay to determine their full potential in diagnosis of this devastating disease. 该研究的下一步就是使用这些抗体进行敏感的筛检试验,以确定他们对这种致命疾病的潜在诊断能力。

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