摘要

Recent pharmacological studies have shown that perivascular nerves can influence the development and function of vascular endothelial cells (ECs). However, morphological studies have not yet been carried out to investigate whether these functional changes are associated with changes in vasoactive substances in ECs. We used postembedding electron microscopy (EM) triple gold-labeling immunocytochemistry to study the effects of short-term sympathectomy (3 days after 6-hydroxydopamine [6-OHDA] treatment) and long-term sympathectomy (guanethidine and 8 days after 6-OHDA) on the distribution of vasoactive substances in ECs of the rat thoracic aorta. The post-embedding immunocytochemistry, which can detect levels of label in individual cells, showed that there was a significant decrease in endothelial NO synthase (NOS3)-labeled, serotonin (5-HT)-labeled, and substance P (SP)-labeled, but a significant increase in endothelin-1 (ET-1)-labeled, gold particles in ECs after long-term, but not after short-term (3-day), sympathectomy. In conclusion, our results show that long-term sympathectomy causes an increase in ET-1 and decrease in NOS3, 5-HT, and SP immunoreactivity in ECs of the thoracic aorta. Our data also indicate that postembedding EM triple gold-labeling immunocytochemistry is a valuable technique for quantitative studies of the content of vasoactive substances in ECs.

译文

最近的药理学研究表明，血管周围神经可以影响血管内皮细胞 (ECs) 的发育和功能。然而，尚未进行形态学研究来调查这些功能变化是否与 ECs 中血管活性物质的变化有关。我们使用包埋后电子显微镜 (EM) 三重金标记免疫细胞化学来研究短期交感神经切除术的效果 (6-羟基多巴胺 [6-OHDA] 治疗后 3 天) 和长期交感神经切除术 (6-ohda 后 8 天) 对大鼠胸主动脉 ECs 血管活性物质分布的影响。嵌入后的免疫细胞化学可以检测单个细胞的标记水平，显示内皮一氧化氮合酶 (NOS3) 标记、血清素 (5-HT) 标记、和物质 P (SP)-标记，但 endothelin-1 (ET-1)-标记的显著增加，金颗粒在 ECs 后长期，但不是短期(3 天)，交感神经切除术。总之，我们的结果表明，长期交感神经切除术会导致胸主动脉 ECs 中 ET-1 的增加和 NOS3 、 5-HT 和 SP 免疫反应性的降低。我们的数据还表明，嵌入后 EM 三重金标记免疫细胞化学是定量研究 ECs 中血管活性物质含量的有价值的技术。