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首页 > 医学词汇大全 > Carotid Endarterectomy
Carotid Endarterectomy

神经

关键词神经 治疗方法 颈动脉粥样硬化病

词汇介绍

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解析

carotid   英 [kə'rɒtɪd]  美 [kə'rɑtɪd] 

       adj. (与)颈动脉(有关)的

  n. 颈动脉

     carotid atherosclerosis 颈动脉粥样硬化;颈动脉硬化

  carotid compression 压颈动脉试验

  Carotid plaque 颈动脉斑块;颈动脉粥样斑块

     To evaluate the effect of diclofenac potassium in the treatment of carotid inflammation. 评价应用双氯芬酸钾片治疗颈动脉炎的效果。

 

endarterectomy   英 [,endɑːtə'rektəmɪ]  美 [,ɛndɑrtə'rɛktəmi] 

       n. 动脉内膜切除术

同根词   endarteritis  n. endarteritis [内科] 动脉内膜炎

      This study developed a multivariable model predicting the risk of death or stroke within 30 days of carotid endarterectomy. 该研究以多变量模型预测的颈动脉内膜切除术30天内死亡或卒中的风险。

概述

病情评估颈动脉内膜切除的对象是颈动脉粥样硬化。颈动脉粥样硬化病人临床可表现为暂时缺血性发作(TIA)、可逆缺血性神经功能缺陷(RIND),进展性卒中(PS)或完全卒中(CS)。医师应认真了解病史,仔细查体,结合影像学检查资料,对病情作出恰当的评估。有些病人可能毫无症状,只是因为在例行的查体中闻及颈部血管杂音,或作辅助检查(如超声)方发现颈动脉粥样硬化。无论症状性亦或无症状性颈动脉粥样硬化。凡拟行颈动脉内膜切除者,术前均应了解可能影响手术结果的因素,如年龄、血压、血糖、心肺功能及神经系统情况,颈动

Flow Cytometry-Based Characterization of Mast Cells in Human Atherosclerosis复制标题

基于流式细胞术的人类动脉粥样硬化肥大细胞的表征

发表时间:2019-04-09

影响因子:5.7

作者: Eva Kritikou

期刊:Cells

The presence of mast cells in human atherosclerotic plaques has been associated with adverse cardiovascular events. Mast cell activation, through the classical antigen sensitized-IgE binding to their characteristic Fcε-receptor, causes the release of their cytoplasmic granules. These granules are filled with neutral proteases such as tryptase, but also with histamine and proinflammatory mediators. Mast cells accumulate in high numbers within human atherosclerotic tissue, particularly in the shoulder region of the plaque. These findings are largely based on immunohistochemistry, which does not allow for the extensive characterization of these mast cells and of the local mast cell activation mechanisms. In this study, we thus aimed to develop a new flow-cytometry based methodology in order to analyze mast cells in human atherosclerosis. We enzymatically digested 22 human plaque samples, collected after femoral and carotid endarterectomy surgery, after which we prepared a single cell suspension for flow cytometry. We were able to identify a specific mast cell population expressing both CD117 and the FcεR, and observed that most of the intraplaque mast cells were activated based on their CD63 protein expression. Furthermore, most of the activated mast cells had IgE fragments bound on their surface, while another fraction showed IgE-independent activation. In conclusion, we are able to distinguish a clear mast cell population in human atherosclerotic plaques, and this study establishes a strong relationship between the presence of IgE and the activation of mast cells in advanced atherosclerosis. Our data pave the way for potential therapeutic intervention through targeting IgE-mediated actions in human atherosclerosis.

译文

人动脉粥样硬化斑块中肥大细胞的存在与不良心血管事件有关。通过经典抗原致敏IgE与其特征性Fcε-受体结合,肥大细胞活化导致其细胞质颗粒的释放。这些颗粒填充有中性蛋白酶,例如类胰蛋白酶,但也含有组胺和促炎介质。肥大细胞在人类动脉粥样硬化组织中大量积聚,特别是在斑块的肩部区域。这些发现主要基于免疫组织化学,其不允许广泛表征这些肥大细胞和局部肥大细胞激活机制。在这项研究中,我们因此旨在开发一种新的基于流式细胞术的方法,以分析人类动脉粥样硬化中的肥大细胞。我们酶法消化22个人类斑块样本,在股动脉和颈动脉内膜切除术后收集,之后我们制备了单细胞悬液用于流式细胞术。我们能够鉴定表达CD117和FcεR的特定肥大细胞群,并观察到大多数斑块内肥大细胞基于其CD63蛋白表达而被激活。此外,大多数活化的肥大细胞在其表面结合有IgE片段,而另一部分显示IgE非依赖性激活。总之,我们能够区分人类动脉粥样硬化斑块中明确的肥大细胞群,本研究建立了IgE的存在与晚期动脉粥样硬化中肥大细胞活化之间的密切关系。我们的数据通过针对人类动脉粥样硬化中IgE介导的作用为潜在的治疗干预铺平了道路。