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首页 > 医学词汇大全 > Catecholamine
Catecholamine

内分泌

关键词内分泌 细胞因子 神经递质

词汇介绍

拓展阅读

解析

catecholamine   英 /ˌkætəˈkɒləˌmiːn/   美 /ˌkætəˈtʃoʊləˌmɪn; ˌkætəˈkoʊləˌmin/

释    义   n. 儿茶酚胺

例    句   Primary culture of human embryonic hepatocytes was employed to study the effects of catecholamine and thrombin on the secretion of histidine rich glycoprotein(HRG). 采用人胎肝细胞原代培养方法,观察儿茶酚胺和凝血酶对肝细胞富组氨酸糖蛋白(HRG)分泌的影响。

概述

儿茶酚胺(简称CA)是一种单胺神经递质,一个有机化合物,其具有儿茶酚(苯具有两个羟基的侧基彼此相邻)和副作用链胺。邻苯二酚可以是一个自由分子,也可以是较大分子的取代基,在这里它代表1,2-二羟基苯甲基。儿茶酚胺来源于氨基酸 酪氨酸,酪氨酸来源于饮食以及苯丙氨酸的合成。儿茶酚胺是水溶性的,在循环中与血浆蛋白结合50%。儿茶酚胺中包括肾上腺素,去甲肾上腺素和多巴胺。酪氨酸选自苯丙氨酸通过创建羟化通过酶苯丙氨酸羟化酶,酪氨酸也直接从饮食蛋白中摄取。生物合成多巴胺是由DOPA合成的第一种儿茶酚胺。反

Novel protein kinase C participates catecholamine biosynthesis and immunocompetence modulation in haemocytes of Litopenaeus vannamei 复制标题

新蛋白激酶C参与凡纳滨对虾血细胞儿茶酚胺生物合成和免疫活性调节

发表时间:2019-09-26

影响因子:3.1

作者: Chin-Chyuan Chang

期刊:Dev Com Immunol

Through specific signal transduction pathways, multiple cellular processes including differentiation, cell growth, secretion and muscle contraction were regulated by varied isotypes of protein kinase C (PKC) following the difference of their tissue distributions and localization within the cells. Cells response to signals through phosphorylation of critical targeted proteins following triggering the turnover of inositol phospholipids (IPs), which generates diacylglycerol (DG) and mobilizes Ca2+, are activated to result in the cell activation. Upon elucidating the primary structure and biochemical properties, and the difference of demand on DGs, phorbol esters, acidic phospholipids such as phosphatidylserine (PS), and the presence of Ca2+, there were three classes including classical or conventional (c)PKC, novel (n)PKC, and atypical (a)PKC isotypes reviewed by Ohno and Nishizuka. Base on the well preserved catalytic and regulatory domains peptidic sequences of the different vertebrate PKC families, the use of antibodies could be the strategy for detecting specific protein with antibodies obtained against mammalian molecules in invertebrates. In mollusks, different isotypes of cPKC (DG- and Ca2+-dependent) and nPKC (DGdependent and Ca2+-independent) were detected in the cytosolic fraction in the adductor and retractor muscles of Mytilus galloprovincialis by immunoblot analysis. In crustaceans, PKCα (a cPKC isotype) in regulating vitellogenin uptake in the ovary of Cherax quadricarinatus was identified by rabbit polyclonal anti-PKC isoenzyme antibodies. In Penaeus monodon, using mammalian PKC antibodies, PKCδ (an nPKC isotype) was purified from the hepatopancreas. In addition, the preserved domains sequences of aPKC in Macrobrachium rosenbergii and nPKC in Litopenaeus vannamei were isolated using designed degenerate primers and the rapid amplification of cDNA ends. Using staurosporine (a PKC inhibitor) and phorbol 12- myristate 13-acetate (PMA, a PKC activator), Johansson and Söderhäll reported that the degranulation of crayfish granular blood cells and the release of the proPO activating system can be mediated by two endogenous ligands, a 76-kDa cell adhesion protein (peroxinectin) or a β-l,3-glucan binding protein through PKC signaling transduction. Moreover, Ng et al. indicated that extracellular traps for phagocytosis were revealed in L. vannamei responding to PMA stimulation. These revealed that not only the structural domains of PKC isotypes but also the pharmaceuticals for PKC might be similar in either vertebrates or invertebrates.

译文

通过特定的信号转导途径,多种多样的细胞过程(包括分化,细胞生长,分泌和肌肉收缩)受蛋白质激酶C(PKC)的不同同种型所调节,这取决于它们的组织分布和在细胞内的定位差异。在触发肌醇磷脂(IPs)的转换后,细胞通过关键靶蛋白的磷酸化对信号作出响应,从而产生二酰基甘油(DG)和动员Ca2 +,从而激活细胞。在阐明其主要结构和生化特性以及对DG,佛波酯,酸性磷脂(如磷脂酰丝氨酸(PS))和Ca2 +的需求差异之后,分为三类,包括经典或常规(c)PKC,新颖( n)PKC和由Ohno和Nishizuka审查的非典型(a)PKC同种型。基于保存良好的不同脊椎动物PKC家族的催化和调节域肽序列,使用抗体可能是利用针对无脊椎动物哺乳动物分子的抗体检测特定蛋白质的策略。在软体动物中,通过免疫印迹分析法检测了盖氏菌Mytilus galloprovincialis内收肌和牵开肌的胞质部分中cPKC(依赖于DG和Ca 2+)和nPKC(依赖于DG和Ca 2+)的不同同种型。在甲壳类动物中,通过兔多抗PKC同工酶抗体鉴定了调节Cherax quadricarinatus卵巢中卵黄蛋白原摄取的PKCα(一种cPKC同种型)。在斑节对虾中,使用哺乳动物PKC抗体从肝胰腺中纯化PKCδ(nPKC同种型)。另外,使用设计的简并引物分离了罗氏沼虾中aPKC的保留结构域序列和凡纳滨对虾中的nPKC的保留结构域序列,并快速扩增了cDNA末端。 Johansson和Söderhäll使用星形孢菌素(一种PKC抑制剂)和佛波12-肉豆蔻酸酯13-乙酸酯(PMA,一种PKC活化剂),报道了小龙虾颗粒血细胞的脱粒作用和proPO活化系统的释放可以由两个内源性配体介导通过PKC信号转导来表达76-kDa细胞粘附蛋白(过氧化物酶)或β-1,3-葡聚糖结合蛋白。此外,吴等人。指出在对PMA刺激作出反应的南美白对虾中发现了吞噬作用的细胞外陷阱。这些揭示不仅在脊椎动物或无脊椎动物中PKC同种型的结构域而且PKC的药物可能相似。