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High density lipoprotein

内分泌

关键词内分泌 临床研究术语 脂质运输

词汇介绍

拓展阅读

解析

high   英 /haɪ/

释    义   adj. 高的;含某物多的;上层的;价值高的,高级的;崇高的;高音调的;

               adv. (high) 在高处;(成本或价值)高,奢侈地;(数量)高;音调高

               n. 高水平;高气压区;最高气温;天空;极大欢乐


例    句   I looked down from the high window. 我从高高的窗户往下看。

 

density   英 /'densɪtɪ/   美 /'dɛnsəti/

释    义   n. 密度

同根词   adj. dense 稠密的;浓厚的;愚钝的

               adv. densely 浓密地;密集地

               n. denseness 密集;稠密;浓厚

例    句   This wave of excess density is sound. 这种过剩密度的波就是声波。

 

lipoprotein   英 /'lɪpə(ʊ),prəʊtiːn; 'laɪ-/   美 /'lɪpəprotin/

释    义   n. [生化] 脂蛋白

例    句   This article reviews the roles of lipoprotein transport system in plasma lipid metabolism in poultry. 本文详细讨论了脂蛋白转运体系在家禽脂类代谢中的作用。

概述

高密度脂蛋白(HDL)是五大脂蛋白之一,脂蛋白是由多种蛋白质组成的复杂颗粒,它们在细胞间质中输送身体所有脂肪分子(脂质),它们通常由每个粒子80-100个蛋白质组成,并且每个粒子运输多达数百个脂肪分子。检测通常对替代值HDL-C(即与ApoA-1/HDL颗粒相关的胆固醇)进行血液测试。在健康个体中,大约30%的血液胆固醇以及其他脂肪由HDL携带。这通常与估计在低密度脂蛋白颗粒LDL中携带的胆固醇的量形成对比,并称为LDL-C。HDL颗粒从细胞中去除脂肪和胆固醇,包括动脉壁粥样斑块内的脂肪和胆

Endothelial lipase increases antioxidative capacity of high-density lipoprotein.复制标题

内皮脂肪酶增加高密度脂蛋白的抗氧化能力。

发表时间:2019-10-01

影响因子:4.4

作者: Irene Schilcher

期刊:Biochim Biophys Acta Mol Cell Biol Lipids

Endothelial lipase (EL) is a strong determinant of structural and functional properties of high-density lipoprotein (HDL). We examined whether the antioxidative capacity of HDL is affected by EL. EL-modified HDL (EL-HDL) and control EV-HDL were generated by incubation of HDL with EL- overexpressing or control HepG2 cells. As determined by native gradient gel electrophoresis, electron microscopy, and small-angle X-ray scattering EL-HDL is smaller than EV-HDL. Mass spectrometry revealed an enrichment of EL-HDL with lipolytic products and depletion of phospholipids and triacylglycerol. Kinetics of conjugated diene formation and HPLC-based malondialdehyde quantification revealed that EL-HDL exhibited a significantly higher resistance to copper ion-induced oxidation and a significantly higher capacity to protect low-density lipoprotein (LDL) from copper ion-induced oxidation when compared to EV-HDL. Depletion of the lipolytic products from EL-HDL abolished the capacity of EL-HDL to protect LDL from copper ion-induced oxidation, which could be partially restored by lysophosphatidylcholine enrichment. Proteomics of HDL incubated with oxidized LDL revealed significantly higher levels of methionine 136 sulfoxide in EL-HDL compared to EV-HDL. Chloramine T (oxidizes methionines and modifies free thiols), diminished the difference between EL-HDL and EV-HDL regarding the capacity to protect LDL from oxidation. In absence of LDL small EV-HDL and EL-HDL exhibited higher resistance to copper ion-induced oxidation when compared to respective large particles. In conclusion, the augmented antioxidative capacity of EL-HDL is primarily determined by the enrichment of HDL with EL-generated lipolytic products and to a lesser extent by the decreased HDL particle size and the increased activity of chloramine T-sensitive mechanisms.

译文

内皮脂肪酶(EL)是高密度脂蛋白(HDL)结构和功能特性的强决定因素。我们检查了HDL的抗氧化能力是否受到EL的影响。通过将HDL与EL-过表达或对照HepG2细胞一起温育,产生EL-修饰的HDL(EL-HDL)和对照EV-HDL。通过天然梯度凝胶电泳,电子显微镜和小角度X射线散射EL-HDL测定,小于EV-HDL。质谱分析显示EL-HDL富含脂肪分解产物并且磷脂和三酰基甘油耗尽。共轭二烯形成和基于HPLC的丙二醛定量的动力学表明,与以下相比,EL-HDL对铜离子诱导的氧化具有显着更高的抗性,并且显着更高的保护低密度脂蛋白(LDL)免受铜离子诱导的氧化的能力。 EV-HDL。从EL-HDL中消除脂肪分解产物消除了EL-HDL保护LDL免受铜离子诱导的氧化的能力,其可通过溶血磷脂酰胆碱富集而部分恢复。与EV-HDL相比,与氧化LDL一起孵育的HDL蛋白质组学显示EL-HDL中甲硫氨酸136亚砜的水平显着更高。氯胺T(氧化蛋氨酸并改变游离硫醇),减少了EL-HDL和EV-HDL之间关于保护LDL免受氧化的能力的差异。在不存在LDL的情况下,与各个大颗粒相比,小的EV-HDL和EL-HDL表现出更高的对铜离子诱导的氧化的抗性。总之,EL-HDL的增强抗氧化能力主要由HDL与EL产生的脂肪分解产物的富集决定,并且在较小程度上由降低的HDL颗粒大小和氯胺T敏感机制的活性增加决定。