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Influence of lipopolysaccharide on proinflammatory gene expression in human corneal, conjunctival and meibomian gland epithelial cells.
脂多糖对人角膜、结膜和睑板腺上皮细胞促炎基因表达的影响。
Gene expression Inflammation Lipopolysaccharide Ocular surface epithelial cells
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摘要

PURPOSE:Lipopolysaccharide (LPS), a bacterial endotoxin, is known to stimulate leuokotriene B4 (LTB4) secretion by human corneal (HCECs), conjunctival (HConjECs) and meibomian gland (HMGECs) epithelial cells. We hypothesize that this LTB4 effect represents an overall induction of proinflammatory gene expression in these cells. Our objective was to test this hypothesis.
METHODS:Immortalized HCECs, HConjECs and HMGECs were cultured in the presence or absence of LPS (15 μg/ml) and ligand binding protein (LBP; 150 ng/ml). Cells were then processed for RNA isolation and the analysis of gene expression by using Illumina BeadChips, background subtraction, cubic spline normalization and GeneSifter software.
RESULTS:Our findings show that LPS induces a striking increase in proinflammatory gene expression in HCECs and HConjECs. These cellular reactions are associated with a significant up-regulation of genes associated with inflammatory and immune responses (e.g. IL-1β, IL-8, and tumor necrosis factor), including those related to chemokine and Toll-like receptor signaling pathways, cytokine-cytokine receptor interactions, and chemotaxis. In contrast, with the exception of Toll-like signaling and associated innate immunity pathways, almost no proinflammatory ontologies were upregulated by LPS in HMGECs.
CONCLUSIONS:Our results support our hypothesis that LPS stimulates proinflammatory gene expression in HCECs and HConjECs. However, our findings also show that LPS does not elicit such proinflammatory responses in HMGECs.

译文

目的: 脂多糖 (LPS) 是一种细菌内毒素,已知能刺激人角膜 (HCECs) 、结膜 (HConjECs) 和睑板腺 (mmgecs) 分泌亮三烯 B4 (LTB4) 上皮细胞。我们假设这种 LTB4 效应代表了这些细胞中促炎基因表达的整体诱导。我们的目标是检验这个假设。
方法: 在 LPS (15 μ g/ml) 和配体结合蛋白 (LBP; 150 μ g/ml) 存在或不存在的情况下培养永生化的 HCECs 、 hconecs 和 mmgecs。然后使用 Illumina BeadChips 、背景减法、三次样条归一化和 GeneSifter 软件处理细胞进行 RNA 分离和基因表达分析。
结果: 我们的研究结果表明,LPS 诱导 HCECs 和 hconecs 中促炎基因表达的显著增加。这些细胞反应与与炎症和免疫反应相关的基因显著上调相关 (例如 IL-1 β 、 IL-8 和肿瘤坏死因子),包括与趋化因子和 Toll 样受体信号通路、细胞因子-细胞因子受体相互作用和趋化性相关的信号通路。相比之下,除了 Toll 样信号和相关的先天免疫通路之外,在 mmgecs 中,LPS 几乎没有上调促炎本体。
结论: 我们的结果支持我们的假设,即 LPS 刺激 HCECs 和 HConjECs 中的促炎基因表达。然而,我们的研究结果也表明 LPS 不会在 mmgecs 中引起这种促炎反应。

Lipopolysaccharide

内分泌 内毒素 临床研究术语
概述  :  

脂多糖(LPS),也称为脂聚糖和内毒素,是大的分子组成的脂质和多糖的O-抗原,它们存在于革兰氏阴性细菌的外膜中。保持在细菌细胞内,并且仅在破坏细菌细胞壁后才释放。随后的研究表明,从革兰氏阴性菌中释放LPS 不一定需要破坏细菌细胞壁,相反,LPS作为膜囊泡运输的正常生理活性的一部分以下列形式分泌:细菌外膜囊泡(OMV),也可能包含其他毒力因子和蛋白质。组成O抗原:LPS中包含的重复聚糖聚合物称为细菌的O抗原,O多糖或O侧链。O抗原连接至核心寡糖,并包含LPS分子的最外层结构域。O链的组成因菌株

Lipopolysaccharide   英 /ˌlʌɪpəʊpɒlɪˈsakərʌɪd/   美 /ˌlʌɪpəʊpɒlɪˈsakərʌɪd/

释    义   n. [有化] 脂多糖

例    句   It recognizes lipopolysaccharide, a component of the outer membrane of Gram-negative bacteria. 它识别广泛存在革兰氏阴性菌细胞外膜上的脂多糖。

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