摘要

BACKGROUND & AIMS:Silymarin, an extract from the seeds of the milk thistle plant Silybum marianum, has been used for centuries for the treatment of chronic liver diseases. Despite common use by patients with hepatitis C in the United States, its clinical efficacy remains uncertain. The goal of this study was to determine whether silymarin has in vitro effects on immune function that might have implications for its potential effect on hepatitis C virus (HCV)-induced liver disease.
METHODS:Freshly isolated peripheral blood mononuclear cells (PBMC) and T cells from HCV-infected and uninfected subjects were tested in vitro for responses to nonspecific and antigenic stimulation in the presence and absence of a standardized preparation of silymarin (MK001).
RESULTS:Minimal MK001 toxicity on PBMC was found at concentrations between 5 and 40 microg/mL. MK001 dose dependently inhibited the proliferation and secretion of tumor necrosis factor (TNF)-alpha, interferon (IFN)-gamma, and interleukin (IL)-2 by PBMC stimulated with anti-CD3. In addition, MK001 inhibited proliferation by CD4(+) T cells to HCV, Candida, and tetanus protein antigens and by HLA-A2/HCV 1406-1415-specific CD8(+) T cells to allogeneic stimulation. MK001 inhibited T-cell TNF-alpha and IFN-gamma cytokine secretion to tetanus and Candida protein antigens. Finally, MK001 inhibited nuclear factor-kappaB transcriptional activation after T-cell receptor-mediated stimulation of Jurkat T cells, consistent with its ability to inhibit Jurkat T-cell proliferation and secretion of IL-2.
CONCLUSIONS:Silymarin's ability to inhibit the proliferation and proinflammatory cytokine secretion of T cells, combined with its previously described antiviral effect, suggests a possible mechanism of action that could lead to clinical benefit during HCV infection.

译文

背景与目的: 水飞蓟素是一种从水飞蓟种子中提取的植物,几个世纪以来一直被用于治疗慢性肝病。尽管美国丙型肝炎患者普遍使用,但其临床疗效仍不确定。这项研究的目的是确定水飞蓟素是否对免疫功能有体外影响,这可能对丙型肝炎病毒 (丙型肝炎病毒) 诱导的肝病有潜在影响。
方法: 新鲜分离的外周血单个核细胞 (PBMC) 和来自丙型肝炎病毒感染和未感染的受试者的 T 细胞在体外进行测试,在水飞蓟素 (MK001) 的标准化制备的存在和不存在的情况下对非特异性和抗原刺激的反应。
结果: 发现 MK001 对 PBMC 的毒性最小,浓度在 5 至 40 微克/毫升之间。MK001 剂量依赖性地抑制 anti-CD3 刺激的 PBMC 的肿瘤坏死因子 (TNF)-α 、干扰素 (IFN)-γ 和白细胞介素 (IL)-2 的增殖和分泌。此外,MK001 抑制 CD4 () T 细胞对丙型肝炎病毒、念珠菌和破伤风蛋白抗原的增殖,以及 HLA-A2/丙型肝炎病毒 1406-1415 特异性 CD8 () T 细胞对同种异体刺激的增殖。MK001 抑制 T 细胞 TNF-α 和 IFN-γ 细胞因子分泌到破伤风和念珠菌蛋白抗原。最后,MK001 在 T 细胞受体介导的 Jurkat T 细胞刺激后抑制核因子-kappaB 转录激活,与其抑制 Jurkat T 细胞增殖和分泌 IL-2 的能力一致。
结论: 水飞蓟素抑制 T 细胞增殖和促炎细胞因子分泌的能力,结合其先前描述的抗病毒作用, 提出了一种可能的作用机制,该机制可能导致丙型肝炎病毒感染期间的临床益处。

Silymarin

消化 肝脏疾病治疗 药物
概述  :  

水飞蓟是菊科水飞蓟属草本植物,原产于南欧、北非。水飞蓟是一种欧洲民间草药,早在公元前四世纪西方就将其提取物用于肝胆疾病的治疗。水飞蓟素( silymarin)是从水飞蓟种子中提取的一种新型黄酮类化合物,主要成分有水飞蓟宾( silybin/silibinin)、异水飞蓟宾( isosilybin)、水飞蓟宁( silydianin)和水飞蓟亭( silychristin)等。其中,以水飞蓟宾含量最高,生物学活性也最强。基础和临床研究证实,水飞蓟宾具有保肝、抗炎、免疫调节、降血脂和抗氧化等作用

silymarin  /səˈlaɪmərɪn/

释义   n. 水飞蓟;水飞蓟素;水飞蓟宾

例句   OBJECTIVE: To study the pharmacological mechanism of silymarin on human cervical cancer cell. 目的:探讨水飞蓟素对人子宫颈癌细胞的药理作用机制。


请扫描右侧二维码,免费查看词汇专业知识背景