消化
词汇介绍
拓展阅读
解析
lipase 英 [ˈlaɪpeɪz; ˈlɪpeɪz] 美 [ˈlaɪpeɪs,ˈlɪpeɪs]
释义 n. [生化] 脂肪酶;脂肪分解酵素
例句 The microbial lipase could be extensively used in food, fine chemistry, and medicine industry because it could catalyze decomposition, synthesis, and exchange of the esters. 微生物脂肪酶可以催化酯类化合物的分解、合成和酯交换,因而被广泛应用于食品、精细化工和医药等工业中。
概述
概述
脂肪酶(lipase,LPS/LIP)又称三脂酰甘油酯酰水解酶、甘油三酯酶,是胰腺外分泌酶。是一组特异性较低的脂肪水解酶类,主要来源于胰腺,其次为胃及小肠,能水解多种含长链脂肪酸的甘油酯。通常胰腺以等量分泌脂肪酶及共脂肪酶进入循环,但因共脂肪酶相对分子量较小,可以从肾小球滤出,急性胰腺炎时,共脂肪酶/脂肪酶比例下降。LPS能分解长链脂肪酸三酰甘油。血液中的LPS主要来自胰腺,少量来自胃粘膜,血清LPS是急性胰腺炎的重要辅助诊断指标。
检测方法
测定方法有滴定法;比浊法;速率法;偶联法;色原底物法。目前应用较多的是色原底物法,速率法和酶比色法。
参考值
偶联法:1~54 U/L,色原底物法:13~63 U/L;比浊法:36~160 U/L。不同方法测定结果可能有一定差异,各实验室应验证所引用参考区间或建立本实验室的适宜参考区间。
注意事项
1)测定标本可用血清或肝素抗凝血浆,但不能用依他酸(ED-TA)抗凝的血浆,因对测试有干扰。
2)抽血后4h内分离血清或血浆,20~25℃可稳定24h,4℃可稳定5d。
3)胆红素可增加此酶活性,故黄疸标本可使测定结果偏高。
4)血红蛋白可抑制脂肪酶活性,故溶血标本可使测定结果降低。
临床意义与评价
1)主要用于急性胰腺炎的诊断及与其他原因急腹症的鉴别诊断;
2)急性胰腺炎时,血清LPS检测的特异性高于血清AMY,二者灵敏性相似,同时检测灵敏度可提高到95%;发病后4~8h内血清脂肪酶活性升高,24h达峰值,一般持续8~14天。
3)血清LPS检测对胰腺炎晚些时间的诊断更有意义;
4)胰腺癌、胆管癌、慢性胰腺炎、肝癌时可增高;
5)骨折、软组织损伤、手术等引起的脂肪组织破坏及少数乳腺癌可稍增高;
6)降低常见于胰腺癌、胰腺导管阻塞、儿童囊性纤维化等。
藜麦和胡芦巴提取物在体外传统条件或肠道模拟条件下对胰脂肪酶和 α-淀粉酶的抑制作用
发表时间:2019-01-01
影响指数:5.4
作者: Teresa Herrera
期刊:Food Chemistry
Ethanol extracts (EE) from fenugreek and quinoa seeds with different total content of inhibitory compounds (TIC, total saponin plus phenolic) were prepared with and without concentration of TIC (CEE -concentrated EE-, and EE, respectively). Their inhibitory activity on pancreatic lipase and α-amylase was assessed by traditional in vitro methods (with or without orbital shaking), and by simulating intestinal digestion. CEE contained higher contents of TIC than EE, being fenugreek superior to quinoa (p < 0.001). The extracts inhibited enzymes in a dose-dependent manner, CEE extracts being stronger (fenugreek for lipase -p = 0.009-, and quinoa for α-amylase -p < 0.001-). Shaking did not impact the activity. Intestinal conditions worsened the inhibition of lipase, but slightly catalyzed the α-amylase. Longer times of reaction worsened activities. The importance of assessing the inhibitory activity of extracts under simulated intestinal conditions is concluded, being fenugreek more interesting than quinoa, especially against pancreatic lipase.
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