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Lipase

消化

关键词消化 临床研究术语 胰腺炎

词汇介绍

拓展阅读

解析

lipase 英 [ˈlaɪpeɪz; ˈlɪpeɪz] 美 [ˈlaɪpeɪs,ˈlɪpeɪs]

释义   n. [生化] 脂肪酶;脂肪分解酵素

例句   The microbial lipase could be extensively used in food, fine chemistry, and medicine industry because it could catalyze decomposition, synthesis, and exchange of the esters. 微生物脂肪酶可以催化酯类化合物的分解、合成和酯交换,因而被广泛应用于食品、精细化工和医药等工业中。


概述

脂肪酶(lipase,LPS/LIP)又称三脂酰甘油酯酰水解酶、甘油三酯酶,是胰腺外分泌酶。是一组特异性较低的脂肪水解酶类,主要来源于胰腺,其次为胃及小肠,能水解多种含长链脂肪酸的甘油酯。通常胰腺以等量分泌脂肪酶及共脂肪酶进入循环,但因共脂肪酶相对分子量较小,可以从肾小球滤出,急性胰腺炎时,共脂肪酶/脂肪酶比例下降。LPS能分解长链脂肪酸三酰甘油。血液中的LPS主要来自胰腺,少量来自胃粘膜,血清LPS是急性胰腺炎的重要辅助诊断指标。 检测方法测定方法有滴定法;比浊法;速率法;偶联法

Inhibitory effect of quinoa and fenugreek extracts on pancreatic lipase and α-amylase under in vitro traditional conditions or intestinal simulated conditions复制标题

藜麦和胡芦巴提取物在体外传统条件或肠道模拟条件下对胰脂肪酶和 α-淀粉酶的抑制作用

发表时间:2019-01-01

影响因子:5.4

作者: Teresa Herrera

期刊:Food Chemistry

Ethanol extracts (EE) from fenugreek and quinoa seeds with different total content of inhibitory compounds (TIC, total saponin plus phenolic) were prepared with and without concentration of TIC (CEE -concentrated EE-, and EE, respectively). Their inhibitory activity on pancreatic lipase and α-amylase was assessed by traditional in vitro methods (with or without orbital shaking), and by simulating intestinal digestion. CEE contained higher contents of TIC than EE, being fenugreek superior to quinoa (p < 0.001). The extracts inhibited enzymes in a dose-dependent manner, CEE extracts being stronger (fenugreek for lipase -p = 0.009-, and quinoa for α-amylase -p < 0.001-). Shaking did not impact the activity. Intestinal conditions worsened the inhibition of lipase, but slightly catalyzed the α-amylase. Longer times of reaction worsened activities. The importance of assessing the inhibitory activity of extracts under simulated intestinal conditions is concluded, being fenugreek more interesting than quinoa, especially against pancreatic lipase.

译文

从葫芦巴和藜麦种子中提取总乙醇含量不同的抑制性化合物(TIC,总皂苷和酚类)的乙醇提取物(EE),分别添加和不添加TIC(分别为CEE浓缩的EE-和EE)。通过传统的体外方法(有或没有轨道摇动),并通过模拟肠消化,评估了它们对胰腺脂肪酶和α-淀粉酶的抑制活性。 CEE的TIC含量高于EE,是胡芦巴优于藜麦(p <0.001)。提取物以剂量依赖的方式抑制酶,CEE提取物则更强(脂酶-p = 0.009-,为芬努尔,α-淀粉酶-p <0.001--为藜麦)。摇晃并没有影响活动。肠道条件恶化了对脂肪酶的抑制,但略微催化了α-淀粉酶。反应时间越长,活动越差。总结了评估提取物在模拟肠道条件下的抑制活性的重要性,胡芦巴比藜麦更有趣,尤其是针对胰脂肪酶。