Ultrasonic pretreatment promotes diacylglycerol production from lard by lipase-catalysed glycerolysis and its physicochemical properties.
超声波预处理通过脂肪酶催化甘油解促进猪油生产甘油二酯及其理化性质。
Diacylglycerol Lard Lipase-catalysed glycerolysis Physicochemical property Ultrasonic pretreatment
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摘要

The objective of this study was to evaluate the effect of ultrasonic pretreatment on diacylglycerol (DAG) synthesis by lipase-catalysed glycerolysis of lard and to analyse the physicochemical properties of lard-based DAG. The optimal ultrasonic pretreatment conditions were: Rhizomucor miehei (Lipozyme® RMIM)-to-lard ratio 4:100 (W/W), 45 °C for 5 min, and power 250 W. The lard-based DAG samples for 4 h of glycerolysis reactions with ultrasonic pretreatment (named DAG-U) and 11 h of glycerolysis reactions without ultrasonic pretreatment (named DAG-N) had similar DAG contents and were used for further analysis. The major FA compositions and iodine value of lard, DAG-U and DAG-N were similar. Fourier transform infrared spectroscopy analysis proved that enzymatic glycerolysis with and without ultrasonic pretreatment did not change the structure of the lard. Differential scanning calorimetry analysis showed that the crystallization onset of DAG-U and DAG-N shifted to higher temperatures than that of lard, which indicated that DAG oils accelerated nucleation and crystal growth. X-ray diffraction analysis revealed that both DAG-U and DAG-N contained β' crystal and a substantially lower amount of β crystal. Overall, ultrasonic pretreatment promotes diacylglycerol production from lard through lipase-catalysed glycerolysis, and DAG-U and DAG-N have similar physicochemical properties.

译文

本研究的目的是评估超声波预处理对脂肪酶催化猪油甘油解合成甘油二酯 (DAG) 的影响,并分析猪油基 DAG 的理化性质。超声波预处理的最佳条件是: 木霉 (Lipozyme®RMIM)-猪油比 4: 100 (W/W),45 ℃ 持续 5 分钟,功率 250 W猪油基 DAG 样品用超声波预处理 (命名为 DAG-U) 进行 4 小时的甘油溶解反应,用超声波预处理 (命名为 DAG-N) 进行 11 小时的甘油溶解反应具有相似的 DAG 内容,并用于进一步分析。猪油、 DAG-U 和 DAG-N 的主要 FA 组成和碘值相似。傅里叶变换红外光谱分析表明,超声波预处理和不超声波预处理都不会改变猪油的结构。差示扫描量热分析表明,DAG-U 和 DAG-N 的结晶起始温度比猪油更高,这表明 DAG 油加速成核和晶体生长。X射线衍射分析表明,DAG-U 和 DAG-N 都含有 β '晶体和大量的 β 晶体。总的来说,超声波预处理通过脂肪酶催化甘油解促进猪油生产甘油二酯,DAG-U 和 DAG-N 具有相似的理化性质。

Lipase

消化 胰腺炎 临床研究术语
概述  :  

脂肪酶(lipase,LPS/LIP)又称三脂酰甘油酯酰水解酶、甘油三酯酶,是胰腺外分泌酶。是一组特异性较低的脂肪水解酶类,主要来源于胰腺,其次为胃及小肠,能水解多种含长链脂肪酸的甘油酯。通常胰腺以等量分泌脂肪酶及共脂肪酶进入循环,但因共脂肪酶相对分子量较小,可以从肾小球滤出,急性胰腺炎时,共脂肪酶/脂肪酶比例下降。LPS能分解长链脂肪酸三酰甘油。血液中的LPS主要来自胰腺,少量来自胃粘膜,血清LPS是急性胰腺炎的重要辅助诊断指标。 检测方法测定方法有滴定法;比浊法;速率法;偶联法

lipase 英 [ˈlaɪpeɪz; ˈlɪpeɪz] 美 [ˈlaɪpeɪs,ˈlɪpeɪs]

释义   n. [生化] 脂肪酶;脂肪分解酵素

例句   The microbial lipase could be extensively used in food, fine chemistry, and medicine industry because it could catalyze decomposition, synthesis, and exchange of the esters. 微生物脂肪酶可以催化酯类化合物的分解、合成和酯交换,因而被广泛应用于食品、精细化工和医药等工业中。


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