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Application of DNA amplification to pneumocystosis: presence of serum Pneumocystis carinii DNA during human and experimentally induced Pneumocystis carinii pneumonia.
DNA 扩增在肺孢子虫病中的应用: 在人和实验诱导的肺孢子虫肺炎过程中血清卡氏肺孢子虫 DNA 的存在。

摘要

Pneumocystis carinii pneumonia is a leading cause of morbidity and mortality in patients with the acquired immunodeficiency syndrome (AIDS). Much remains unknown about the basic biology of P. carinii and studies of this infection have been hampered by the lack of cultivation methods. We developed a sensitive and specific assay for P. carinii by utilizing DNA amplification of the P. carinii dihydrofolate reductase (DHFR) gene. By this method, P. carinii DNA was detected in the lungs of rats with experimentally induced P. carinii pneumonia 2 wk before the onset of histopathological changes. DNA amplification analysis of serum demonstrated that by 10 wk of corticosteroid treatment, 12 of 12 (100%) infected rats had circulating DHFR DNA. P. carinii DHFR DNA also was detected in the serum of patients with AIDS and active P. carinii pneumonia (12 of 14 sera collected prospectively). Patients with advanced AIDS but without a history of P. carinii pneumonia were negative by this assay (0 of 6 sera examined). Serum polymerase chain reaction may facilitate investigations into the natural history and epidemiology of P. carinii infection, provide insight into the pathogenesis of parasite dissemination, and offer a useful, noninvasive diagnostic test for the detection of human pneumocystosis.

译文

卡氏肺孢子虫肺炎是获得性免疫缺陷综合征 (艾滋病) 患者发病率和死亡率的主要原因。关于 P.carinii 的基本生物学还有很多未知之处,并且由于缺乏培养方法,对这种感染的研究受到了阻碍。我们利用卡氏菌二氢叶酸还原酶 (DHFR) 基因的 DNA 扩增,建立了卡氏菌的敏感和特异性检测方法。通过这种方法,在组织病理学变化开始前 2 周,在实验性卡氏肺炎大鼠的肺部检测到卡氏疟原虫的 DNA。血清 DNA 扩增分析表明,在皮质类固醇治疗 10 周时,12 只 (100%) 感染大鼠中有 12 只存在循环 DHFR DNA。在患有艾滋病和活动性卡氏疟原虫肺炎的病人的血清中也检测到卡氏疟原虫的 DHFR DNA (前瞻性收集的 14 份血清中的 12 份)。患有晚期艾滋病但没有肺炎支原体肺炎病史的患者,该检测结果为阴性 (6 份血清中检测到 0 份)。血清聚合酶链反应可促进对 P 的自然史和流行病学的研究。卡氏菌感染,提供了对寄生虫传播的发病机理的洞察,并为检测人类肺炎提供了一种有用的、无创的诊断测试。

Pneumocystosis

呼吸 可引起肺孢子菌肺炎 疾病
概述  :  

疾病概述 肺孢子菌病是由潜伏的肺孢子菌,在机体免疫抑制或受损时大量繁殖,破坏肺泡细胞,引起间质性肺炎即肺孢子菌肺炎。肺孢子菌肺炎尤其多见于艾滋病患者,也是其重要的致死原因;而器官移植、免疫抑制药物的广泛开展和应用,是肺孢子菌肺炎发病率上升的另一重要原因。主要临床表现为发热、干咳、进行性呼吸困难等,单纯吸氧不能缓解,经对因治疗后可迅速恢复。 致病因素或病理机制肺孢子菌为毒力较弱,生长缓慢。健康人感染后多无病理损伤,且多呈隐性感染,只有在机体免疫功能缺陷或低下的患者才可能发生显性感染。目前

Pneumocystosis   /njuːməsɪs'təʊsɪs/

释    义   [内科] 肺孢子虫病

例    句   Artemisinin is also a promising potent drug in treatment of cancer, human parasites such as schistosomiasis, pneumocystosis and AIDS complication. 同时青蒿素也是治疗癌症、血吸虫病等人类寄生虫、肺孢子菌病、艾滋病并发症的有效药物。

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