We examined seizure-susceptibility in a Drosophila model of human epilepsy using optogenetic stimulation of ReaChR (red-activatable channelrhodopsin). Photostimulation of the seizure-sensitive mutant parabss1 causes behavioral paralysis that resembles paralysis caused by mechanical stimulation, in many aspects. Electrophysiology shows that photostimulation evokes abnormal seizure-like neuronal firing in parabss1 followed by a quiescent period resembling synaptic failure and apparently responsible for paralysis. The pattern of neuronal activity concludes with seizure-like activity just prior to recovery. We tentatively identify the mushroom body as one apparent locus of optogenetic seizure initiation. The α/β lobes may be primarily responsible for mushroom body seizure induction.

译文

:我们使用ReaChR(红色激活的视紫红质)的光遗传学刺激,在人癫痫的果蝇模型中检查了癫痫发作的易感性。癫痫发作敏感突变体parabss1的光刺激会在许多方面引起类似于机械刺激引起的瘫痪的行为麻痹。电生理学表明,光刺激会引起parabss1异常的癫痫样神经元放电,然后是类似于突触衰竭的静止期,显然是造成瘫痪的原因。神经元活动的模式以恢复前的癫痫样活动结束。我们初步确定蘑菇体是光遗传性癫痫发作的一个明显部位。 α/β瓣可能是引起蘑菇体癫痫发作的主要原因。

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