The promotion potential of phenobarbital (PB) and 3'-methyl-4-dimethylaminoazobenzene (3'-Me-DAB) on liver carcinogenesis after initiation with various doses of diethylnitrosamine (DEN) was assessed using an in vivo short term system. Male F344 rats were pretreated with a single intraperitoneal injection of varying doses of DEN (0, 6, 12, 25, 50, 100 or 200 mg/kg body wt), and 2 weeks later were treated with 0.05% PB or 0.06% 3'-Me-DAB for 6 weeks. All animals were subjected to partial hepatectomy 3 weeks after the DEN treatment. Quantitation of gamma-glutamyltranspeptidase-positive (gamma-GT+) foci revealed a DEN dose-dependent response. Magnitude of promotion by PB and more pronounced by 3'-Me-DAB was, in contrast, strongest at the lower doses of DEN. The results suggest that quantitative differences with regard to initiation level may exist, influencing the promotability of initiated cells.

译文

使用体内短期系统评估了苯巴比妥 (PB) 和3 '-methyl-4-dimethylaminoazobenzene (3'-Me-DAB) 在各种剂量的二乙基亚硝胺 (DEN) 引发肝癌后的促进潜力。用单次腹腔注射不同剂量的DEN (0、6、12、25、50、100或200 mg/kg体重) 预处理雄性F344大鼠,2周后用0.05% PB或0.06% 3 '-Me-DAB治疗6周。所有动物在DEN治疗后3周接受部分肝切除术。Γ-谷氨酰转肽酶阳性 (γ-GT) 病灶的定量显示出DEN剂量依赖性反应。相比之下,在较低剂量的DEN下,PB和3 '-Me-DAB的促进程度最高。结果表明,在起始水平方面可能存在定量差异,从而影响起始细胞的启动子性。

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