Canadian isolates of Leptosphaeria maculans, the causal agent of blackleg of crucifers, were examined for genetic relatedness by the random amplified polymorphic DNA assay. DNA polymorphisms amplified with random decamer primers were used to distinguish three groups of isolates. Group 1 contained all isolates of the virulent pathotype, group 2 contained isolates of the avirulent pathotype from western Canada, and group 3 contained avirulent pathotype isolates from Ontario. These results agreed with other reports which showed many genetic differences between pathotypes and were consistent with the hypothesis that the virulent pathotype was recently introduced into Canada and has diverged relatively little. In contrast, the avirulent pathotype has probably been present in Canada for a longer time and has diverged with geographic isolation. In addition to establishing genetic relationships, DNA fingerprints generated by the random amplified polymorphic DNA assay have potential applications in pathotype identification and blackleg disease management.

译文

:通过随机扩增多态性DNA分析法检测了十字花科黑脚病的致病病原体,加拿大黑斑病杆菌的分离株。用随机十聚体引物扩增的DNA多态性用于区分三类分离株。第1组包含所有毒力致病型的分离株,第2组包含来自加拿大西部的无毒致病型的分离株,第3组包含来自安大略省的无毒致病型的分离株。这些结果与其他报告一致,这些报告显示了致病型之间存在许多遗传差异,并且与强毒型最近被引入加拿大且差异相对较小的假说相符。相反,无毒病原体型可能在加拿大已经存在了较长时间,并且随着地理隔离而有所不同。除了建立遗传关系外,由随机扩增的多态性DNA分析产生的DNA指纹在病理类型识别和黑腿病管理中也有潜在的应用。

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