Paracoccidioides brasiliensis isolates are not homogeneous in their patterns of pathogenicity in animals and adhesion to epithelial cells. During this investigation, genotypic differences were observed between two samples of P. brasiliensis strain 18 yeast phase (Pb18) previously cultured many times, one taken before (Pb18a) and the other after (Pb18b) animal inoculation. Random amplified polymorphic DNA analysis using the primer OPJ4 distinguished Pb18b from Pb18a by one 308 bp DNA fragment, which after cloning and sequencing was shown to encode a polypeptide sequence homologous to the protein beta-adaptin. It is suggested, by comparison to other micro-organisms, that this protein might play an important role in the virulence of P. brasiliensis. This result demonstrates the influence of in vitro subculturing on the genotype of this organism.

译文

:巴西对虾的分离株在动物中的致病性和对上皮细胞的粘附方式不均一。在这项研究过程中,观察到两次培养过多次的巴西假单胞菌菌株18酵母相(Pb18)的基因型差异,一个样品是在接种动物之前(Pb18a),另一个是在接种之后(Pb18b)。使用引物OPJ4进行的随机扩增多态性DNA分析通过一个308 bp DNA片段将Pb18b与Pb18a区别开来,该片段在克隆和测序后显示编码与蛋白质β-adaptin同源的多肽序列。与其他微生物相比,该蛋白质可能在巴西假单胞菌的毒力中起重要作用。该结果证明了体外传代培养对该生物的基因型的影响。

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