Gu/RNA helicase II (Gu/RH-II) is the first reported mammalian nucleolar RNA helicase that is a member of the D-E-A-D (Asp-Glu-Ala-Asp) box family of proteins. It has an ATP-dependent RNA unwinding (helicase) activity and a separate RNA folding activity (introduction of intramolecular secondary structure into single-stranded RNA). To determine which proteins may bind to Gu/RH-II, a yeast two-hybrid system was used. A cDNA which encoded a protein, called Gu/RH-II binding protein or GBP, was isolated and sequenced. The GBP protein is localized to the nucleus in speckled or diffuse nucleoplasmic patterns. The GBP mRNA level is highest in testis, 9- to 49-fold greater than other tissues. When GBP interacts with Gu/RH-II, proteolytic cleavage of Gu/RH-II occurs; the amino-terminal portion of Gu/RH-II is critical for this proteolysis.

译文

:Gu / RNA解旋酶II(Gu / RH-II)是第一个报道的哺乳动物核仁RNA解旋酶,它是D-E-A-D(Asp-Glu-Ala-Asp)盒蛋白家族的成员。它具有ATP依赖的RNA解旋(解旋酶)活性和单独的RNA折叠活性(将分子内二级结构引入单链RNA)。为了确定哪些蛋白质可以与Gu / RH-II结合,使用了酵母双杂交系统。分离并测序了编码称为Gu / RH-II结合蛋白或GBP的蛋白质的cDNA。 GBP蛋白以斑点或弥散的核质模式定位于细胞核。 GBP mRNA水平在睾丸中最高,比其他组织高9到49倍。当GBP与Gu / RH-II相互作用时,会发生Gu / RH-II的蛋白水解切割。 Gu / RH-II的氨基末端部分对于这种蛋白水解至关重要。

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