Background:Ginseng (G) and Ligustrum lucidum Ait (LLA) are core traditional Chinese medicines in treating myelosuppression formula. The present study was designed to profile effect of G and LLA herb pair (G-LLA) on myelosuppressed mice.

Methods:The mice myelosuppression model was established by intraperitoneal (i.p.) injection of cyclophosphamide (Cy). Hematopoietic function of bone marrow was measured by hemopoietic progenitor cell culture and peripheral blood count, and serum hemopoietic factors were tested by enzyme-linked immunosorbent assay. Bone marrow cell cycle was performed by flow cytometry. HPLC was used to measure 20 potential chemical components related to myelosuppression, including ginsenoside Rg1, Re, Rb1, Rc, Rb2, Rb3, Rd, Rk3, Rh4, 20 (S)-Rg3, 20 (R)-Rg3, Rk1, Rg5, salidroside, and so on.

Results:G, LLA, and G-LLA improved the amount of peripheral blood cells and bone marrow cells of myelosuppressed mice (P < 0.01). They significantly increased the colony quantity of colony-forming unit-granulocyte macrophage, burst-forming unit-erythroid, colony-forming unit-erythroid, and colony-forming unit-megakaryocyte and amount of G2/M and S phase cells (P < 0.01). They also significantly decreased the amount of hematopoiesis-related cytokines (P < 0.01). The content of chemical components in G-LLA changed, and the change of rare saponin was the most obvious.

Conclusion:These results show that G-LLA herb pair might produce synergistic or complementary compatibility effects on bone marrow suppression after chemotherapy. It suggests that the substance basis of G-LLA for treating bone marrow suppression may be effective chemical components.

译文

背景 : 人参 (G) 和女贞子 (LLA) 是治疗骨髓抑制配方的核心中药。本研究旨在分析G和LLA草药对 (g-lla) 对骨髓抑制小鼠的作用。
方法 : 通过腹膜内 (i.p.) 建立小鼠骨髓抑制模型) 注射环磷酰胺 (Cy)。通过造血祖细胞培养和外周血计数测定骨髓造血功能,并通过酶联免疫吸附法检测血清造血因子。通过流式细胞术进行骨髓细胞周期。HPLC用于测量与骨髓抑制有关的20种潜在化学成分,包括人参皂苷Rg1,Re,Rb1,Rc,Rb2,Rb3,Rd,Rk3,Rh4,20 (S)-Rg3,20 (R)-Rg3,Rk1,Rg5,红景天苷,等等。
结果 :G,LLA和g-lla改善了骨髓抑制小鼠的外周血细胞和骨髓细胞的数量 (P <0.01)。它们显着增加了集落形成单位-粒细胞巨噬细胞,爆发形成单位-红系,集落形成单位-红系和集落形成单位-巨核细胞的集落数量以及G2/M和S期细胞的数量 (P <0.01)。它们还显著降低了造血相关细胞因子的数量 (P <0.01)。G-lla中化学成分的含量发生了变化,其中稀有皂苷的变化最为明显。
结论 : 这些结果表明,g-lla草药对化疗后的骨髓抑制可能产生协同或互补的相容性作用。提示g-lla治疗骨髓抑制的物质基础可能是有效的化学成分。

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