Nü-zhen-zi, the fruit of Ligustrum lucidum Ait., is one of the most frequently used liver Yin tonifying Chinese herbs for the treatment of liver cancer. However, the effect of Ligustrum lucidum fruit on hepatocarcinoma cells remains unknown. In the present study, we evaluated the effects of a Ligustrum lucidum fruit extract (LLFE) on human hepatocellular carcinoma Bel-7402 cells. The results showed that LLFE inhibited the proliferation of the Bel-7402 cells in a dose- and time-dependent manner. LLFE induced apoptosis in Bel-7402 cells accompanied by activation of caspase-3, -8 and -9. LLFE-induced apoptosis was completely abrogated by a pan caspase inhibitor, Z-VAD-FMK. LLFE treatment also caused a large and flat morphologic cellular change, positive SA-β-gal staining, and G0/G1 phase cell cycle arrest in the Bel-7402 cells, accompanied by upregulation of p21 and downregulation of RB phosphorylation. Specific knockdown of p21 expression by RNA interference partially abrogated LLFE-induced apoptosis, and significantly abrogated LLFE-induced cell senescence. These observations suggest that Nü-zhen-zi is a potential anticancer herb and support the traditional use of Nü-zhen-zi for hepatocarcinoma treatment.

译文

女贞子,女贞子的果实。,是治疗肝癌最常用的肝阴补益中草药之一。然而,女贞子对肝癌细胞的影响仍然未知。在本研究中,我们评估了女贞子果实提取物 (LLFE) 对人肝癌Bel-7402细胞的影响。结果表明,LLFE以剂量和时间依赖性方式抑制Bel-7402细胞的增殖。LLFE诱导Bel-7402细胞凋亡,并伴有caspase-3,-8和-9的激活。LLFE诱导的凋亡被pan caspase抑制剂Z-VAD-FMK完全消除。LLFE处理还引起Bel-7402细胞中大而平坦的形态变化,阳性SA-β-gal染色和G0/G1期细胞周期停滞,伴随着p21的上调和RB磷酸化的下调。通过RNA干扰特异性敲除p21表达,部分消除了LLFE诱导的细胞凋亡,并显着消除了LLFE诱导的细胞衰老。这些观察结果表明,Nü-zhen-zi是一种潜在的抗癌草药,并支持传统使用Nü-zhen-zi治疗肝癌。

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