This study examined the chemical response of Ligustrum lucidum Ait. and Ligustrum lucidum Ait. f. tricolor (Rehd.) Rehd. when exposed to different sources of atmospheric pollutants. Dry weight/fresh weight ratio, specific leaf area (SLA), sulfur content, chlorophyll concentration, carotenoids, soluble proteins, malondialdehyde (MDA) and hydroperoxy conjugated dienes (HPCD) were determined for leaf samples taken from different sites in the city of Córdoba, Argentina. These sites were categorized in terms of their traffic levels and industrial levels. Both L. lucidum as well as L. lucidum f. tricolor accumulated sulfur in their leaves in sites with high traffic levels, the former being the most sensitive to this type of pollution. Ligustrum lucidum exhibited significantly low concentrations of soluble proteins, in sites with high industrial pollution levels. Ligustrum lucidum f. tricolor did not exhibit a significant response to industrial pollution.