Extracellular magnesium ions [Mg2+]o are known to regulate functions of endothelial cells, but whether [Mg2+]o can alter intracellular free ionized magnesium [Mg2+]i in these cells remains unknown. The present studies, using digital imaging microscopy and the Mg2+ fluorescent probe, mag-fura-2, determined effects of elevation of [Mg2+]o on [Mg2+]i in cultured human aortic endothelial cells. With normal Mg2+(1.2 mM)-containing incubation media, [Mg2+]i was 0.51+/-0.04 mM with a heterogeneous distribution. The ratio of [Mg2+]i/[Mg2+]o was 0.52+/-0.07. Elevation of [Mg2+]o up to 4.8 mM increased [Mg2+]i to 0.80+/-0.07 mM in 2-10 min and lowered the ratio of [Mg2+]i/[Mg2+]o to 0.16+/-0.02. Irrespective of the observed increments of [Mg2+]i, a subcellular heterogeneous distribution of [Mg2+]i was always evident in all cells tested. Our results suggest that [Mg2+]o can regulate [Mg2+]i more rapidly than heretofore believed, supporting the hypothesis that extracellular Mg2+ can exert regulatory effects on endothelial cell functions and probably act as extracellular regulatory cations

译文

:已知细胞外镁离子[Mg2] o调节内皮细胞的功能,但是[Mg2] o是否可以改变这些细胞中的细胞内游离离子化镁[Mg2] i,仍然未知。本研究使用数字成像显微镜和Mg2荧光探针mag-fura-2,确定了培养的人主动脉内皮细胞中[Mg2] o升高对[Mg2] i的影响。在含有正常Mg2(1.2 mM)的培养培养基中,[Mg2] i为0.51 / 0.04 mM,分布不均。 [Mg 2] i / [Mg 2] o的比例为0.52 / -0.07。 [Mg2] o升高至4.8 mM会使[Mg2] i在2-10分钟内增加到0.80 /-0.07 mM,并使[Mg2] i / [Mg2] o的比率降低到0.16 /-0.02。不管观察到的[Mg2] i增量如何,在所有测试的细胞中[Mg2] i的亚细胞异质分布总是很明显的。我们的结果表明,[Mg2] o可以比以前认为的更快地调节[Mg2] i,支持以下假设:细胞外Mg2可以对内皮细胞功能发挥调节作用,并且可能充当细胞外调节阳离子

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