DNA nanotechnology takes advantage of the predictability of DNA interactions to build complex DNA-based functional nanoscale structures. However, when DNA functional and responsive units that are based on non-canonical DNA interactions are employed it becomes quite challenging to predict, understand and control their thermodynamics. In response to this limitation, here we demonstrate the use of isothermal urea titration experiments to estimate the free energy involved in a set of DNA-based systems ranging from unimolecular DNA-based nanoswitches to more complex DNA folds (e.g. aptamers) and nanodevices. We propose here a set of fitting equations that allow to analyze the urea titration curves of these DNA responsive units based on Watson-Crick and non-canonical interactions (stem-loop, G-quadruplex, triplex structures) and to correctly estimate their relative folding and binding free energy values under different experimental conditions. The results described herein will pave the way toward the use of urea titration experiments in the field of DNA nanotechnology to achieve easier and more reliable thermodynamic characterization of DNA-based functional responsive units. More generally, our results will be of general utility to characterize other complex supramolecular systems based on different biopolymers.

译文

DNA纳米技术利用DNA相互作用的可预测性来构建基于DNA的复杂功能纳米结构。但是,当使用基于非规范DNA相互作用的DNA功能和响应单元时,预测,理解和控制其热力学变得非常具有挑战性。响应于此限制,在此我们展示了使用等温尿素滴定实验来估算一组基于DNA的系统中涉及的自由能,这些系统的范围从基于单分子DNA的纳米开关到更复杂的DNA折叠(例如适体)和纳米设备。我们在这里提出一套拟合方程,这些方程可以基于沃森-克里克和非规范相互作用(茎-环,G-四链体,三链体结构)分析这些DNA响应单元的尿素滴定曲线,并正确估计它们的相对折叠并在不同的实验条件下结合自由能值。本文所述的结果将为在DNA纳米技术领域中尿素滴定实验的使用铺平道路,以实现基于DNA的功能响应单元的更容易且更可靠的热力学表征。更一般地,我们的结果将可用于表征基于不同生物聚合物的其他复杂的超分子系统。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录