The enzyme activities of the fungus Lasiodiplodia theobromae (L. theobromae) were studied during degradation of benzo[a]pyrene (BaP). The L. theobromae was isolated from a polycyclic aromatic hydrocarbons (PAHs) contaminated soil collected from the Beijing Coking Plant in China and can potentially use BaP as its sole carbon source with a degradation ratio of up to 53% over 10 days. The activities of lignin peroxidase (LiP) and laccase (LAC) could be detected during BaP biodegradation; while manganese peroxidase (MnP) was not detected. Both glucose and salicylic acid enhanced BaP biodegradation slightly. In contrast, the coexistence of phenanthrene (PHE) inhibited BaP degradation. These metabolic substrates all enhanced the secretion of LiP and LAC. The addition of Tween 80 (TW-80) enhanced BaP biodegradation as well as the LiP and LAC activities. At the same time, TW-80 was degraded by the L. theobromae. In addition, the L. theobromae was compared to Phanerochaete chrysosporium (P. chrysosporium), which is a widely studied fungus for degrading PAH. P. chrysosporium was unable to use BaP as its sole carbon source. The activities of LiP and LAC produced by the P. chrysosporium were less than those of the L. theobromae. Additionally, the four intermediates formed in the BaP biodegradation process were monitored using GC-MS analysis. Four metabolite concentrations first increased and then decreased or obtained the platform with prolonged BaP biodegradation time. Therefore, this study shows that the L. theobromae may be explored as a new strain for removing PAHs from the environment.

译文

:在降解苯并[a] py(BaP)的过程中,研究了Lasitheploromae(L. theobromae)真菌的酶活性。 Theobromae分离自从中国北京焦化厂收集的多环芳烃(PAHs)污染的土壤,可以潜在地使用BaP作为唯一的碳源,在10天内降解率高达53%。 BaP生物降解过程中可检测到木质素过氧化物酶(LiP)和漆酶(LAC)的活性。而未检测到锰过氧化物酶(MnP)。葡萄糖和水杨酸均略微增强了BaP的生物降解能力。相反,菲(PHE)的共存抑制了BaP的降解。这些代谢底物均增强了LiP和LAC的分泌。吐温80(TW-80)的添加增强了BaP的生物降解以及LiP和LAC的活性。同时,TW-80被乳杆菌(L. theobromae)降解。另外,将theobromae与Phanerochaete chrysosporium(P. chrysosporium)进行了比较,Phanerochaete chrysosporium是一种广泛研究的降解PAH的真菌。金黄色葡萄球菌不能使用BaP作为唯一的碳源。金孢假单胞菌产生的LiP和LAC的活性要比Theobromae少。此外,使用GC-MS分析监测BaP生物降解过程中形成的四种中间体。四种代谢物浓度先升高然后降低,或者获得了具有延长的BaP生物降解时间的平台。因此,这项研究表明,Theobromae可以作为一种从环境中去除PAHs的新菌株而被探索。

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