AIM:To investigate the expression patterns of TTYH2 in the human colon cancer and colon cancer cell lines and to evaluate the inhibitory effect of small interfering RNA (siRNA) on the expression of TTYH2 in colon cancer cell lines. METHODS:We investigated the expression patterns of TTYH2 in colon cancer, adjacent non-tumorous colon mucosa, and cancer cell lines (DLD-1, caco-2, and Lovo) by RT-PCR. Furthermore, a siRNA plasmid expression vector against TTYH2 was constructed and transfected into DLD-1 and Caco-2 with Lipofectamine 2000. The down regulation of TTYH2 expression was detected by RT-PCR and the role of siRNA in inducing cell proliferation and cell aggregation was evaluated by MTT and aggregation assay. RESULTS:TTYH2 gene expression in colon cancer tissue was significantly up-regulated compared with normal colonic mucosa (1.23 +/- 0.404 vs 0.655 +/- 0.373, P = 0.0103). Colon cancer derived cell lines including DLD-1, Caco-2, and Lovo also expressed high levels of TTYH2. In contrast, transfection with siRNA-TTYH2 significantly inhibited both proliferation and scattering of these cancer cell lines. CONCLUSION:The present work demonstrates, for the first time, that the TTYH2 gene expression is significantly up-regulated in colon cancer. The TTYH2 gene may play an important role in regulating both proliferating and metastatic potentials of colorectal cancer.

译文

目的:研究TTYH2在人结肠癌和结肠癌细胞系中的表达模式,并评估小干扰RNA(siRNA)对TTYH2在结肠癌细胞系中表达的抑制作用。
方法:我们通过RT-PCR研究了TTYH2在结肠癌,邻近的非肿瘤结肠粘膜和癌细胞系(DLD-1,caco-2和Lovo)中的表达模式。此外,构建了针对TTYH2的siRNA质粒表达载体,并用Lipofectamine 2000将其转染到DLD-1和Caco-2中。通过RT-PCR检测了TTYH2表达的下调,并且siRNA在诱导细胞增殖和细胞聚集中的作用为:通过MTT和聚集分析进行评估。
结果:与正常结肠粘膜相比,结肠癌组织中TTYH2基因表达显着上调(1.23 /-0.404对0.655 /-0.373,P = 0.0103)。结肠癌衍生的细胞系(包括DLD-1,Caco-2和Lovo)也表达高水平的TTYH2。相反,用siRNA-TTYH2转染可显着抑制这些癌细胞系的增殖和扩散。
结论:本研究首次证明了TTYH2基因的表达在结肠癌中显着上调。 TTYH2基因可能在调节结直肠癌的增殖和转移潜能中起重要作用。

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