OBJECTIVES:To evaluate the remediation efficiency of Mucor hiemalis by comparing media elimination, uptake, and biotransformation of microcystin-LR with exposure to pure toxin versus a crude bloom extract. RESULTS:With exposure to the extract, the elimination rate of microcystin-LR from the media, which was 0.28 ng MC-LR l-1 h-1, was significantly higher compared to that achieved with exposure to the pure toxin (0.16 ng MC-LR l-1 h-1) after 24 h. However, intracellular breakdown of microcystin-LR was significantly lower in the extract exposed pellets compared to the pure toxin treated fungal pellets over time. This coincided with reduced intracellular glutathione S-transferase activity with crude extract exposure which could be responsible for the detection of only the glutathione conjugate of microcystin-LR. CONCLUSION:This paper signifies the importance of using laboratory exposure scenarios which resemble conditions in nature to fully understand and evaluate remediation efficiency. There is merit in using M. hiemalis for mycoremediation of cyanotoxins in surface waters.

译文

目的:通过比较暴露于纯毒素与粗花粉提取物的微囊藻毒素-LR的培养基清除,吸收和生物转化,评估穆科(Mucor hiemalis)的修复效率。
结果:暴露于提取物后,培养基中微囊藻毒素LR的清除率为0.28ng MC-LR-1-1 h-1,比暴露于纯毒素(0.16ng MC)的清除率显着更高-LR l-1 h-1)24小时后。然而,随着时间的推移,与纯毒素处理过的真菌沉淀相比,暴露于提取物中的沉淀中的微囊藻毒素-LR的细胞内分解显着降低。这与暴露于粗提物中的细胞内谷胱甘肽S-转移酶活性降低相吻合,这可能仅负责检测微囊藻毒素-LR的谷胱甘肽共轭物。
结论:本文表明使用类似于自然条件的实验室暴露方案来充分理解和评估修复效率的重要性。使用hi。m。hiemalis介导地表水中的氰毒素是有好处的。

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