In this report, the ethyl acetate extract and its major constituent, isorhamnetin 3-o-robinobioside (I3-O-Rob), from Nitraria retusa (N. Retusa) leaf extracts were evaluated for their ability to induce apoptosis in human lymphoblastoid cells (TK6). Apoptosis of TK6 cell line was carried out using DNA fragmentation, poly ADP-ribose polymerase (PARP) cleavage with reference to caspase-3 activity. These tested products, from N. Retusa, enhanced the apoptosis effects in the tested cancer cell line. This result was confirmed by DNA fragmentation and PARP cleavage indicating a release of caspase-3 level. Our results indicate that the original ethyl acetate extract and the I3-O-Rob have a great antiproliferative effect on human lymphoblastoid cells (TK6), which may be due to their involvement in the apoptotic pathway.

译文

:在此报告中,评估了白花叶提取物(N. Retusa)叶提取物中的乙酸乙酯提取物及其主要成分异鼠李素3-o-robinobioside(I3-O-Rob)在人淋巴母细胞中诱导凋亡的能力。 (TK6)。参考caspase-3活性,使用DNA片段化,聚ADP-核糖聚合酶(PARP)切割进行TK6细胞系的凋亡。这些来自N. Retusa的被测产品增强了被测癌细胞系中的细胞凋亡作用。 DNA断裂和PARP裂解表明caspase-3水平的释放证实了这一结果。我们的结果表明,原始的乙酸乙酯提取物和I3-O-Rob对人淋巴母细胞(TK6)具有很大的抗增殖作用,这可能是由于它们参与了凋亡途径。

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