Since flavonoids scavenge reactive oxygen species, they may potentially protect against ischemia/reperfusion injury. This study compared the scavenging capacity of specific flavonoids towards different reactive oxygen species. Whether the differential oxidant scavenging capacity correlated with their protective efficacy in ischemia/reperfusion injury of cardiomyocytes was determined. The free radical scavenging capacity of five flavonoids (wogonin, baicalin, baicalein, catechin and procyanidin B2) was analyzed using electron spin resonance spectrometry for 3 radicals: 1,1-diphenyl-2picrylhydrazyl (DPPH), superoxide and hydroxyl radical. A well-established chick cardiomyocyte model of ischemia (1 h)/reperfusion (3 h) was used to evaluate flavonoid-induced protection against ischemia/reperfusion injury in chronic treatment (pretreated 72 h and treated through ischemia/reperfusion) and acute treatment protocols (during ischemia/reperfusion or only at reperfusion). The cell viability was assessed by propidium iodide. The DPPH scavenging was most significant with catechin, followed by procyanidin B2, baicalein, baicalin, and wogonin. The superoxide scavenging was, similarly, most significant with catechin, followed by baicalein, procyanidin B2, and baicalin. For hydroxyl radical, only baicalein showed a significant scavenging capacity (>50% reduction in ESR signal). For the cardiomyocyte studies, all flavonoids but wogonin showed protection against ischemia/reperfusion injury in the chronic treatment protocol. When flavonoids were administered only during ischemia/reperfusion, baicalein, procyanidin B2, and catechin significantly reduced cell death. If flavonoids were administered just at reperfusion, only baicalein and procyanidin B2 had protective effects, and the efficacy was less. Flavonoids possess specific but differential radical scavenging capacity, which, in conjunction with the timing of treatment, affects their protective efficacy in cardiomyocytes exposed to ischemia/reperfusion.

译文

:由于类黄酮清除了活性氧,它们可能潜在地防止缺血/再灌注损伤。这项研究比较了特定类黄酮对不同活性氧的清除能力。确定了不同的清除氧化剂能力与其在心肌细胞的缺血/再灌注损伤中的保护功效相关联。使用电子自旋共振光谱法分析了3种自由基的1,1,1-二苯基-2吡啶并肼基(DPPH),超氧化物和羟基自由基对5种类黄酮(wogonin,黄ical苷,黄ical素,儿茶素和原花青素B2)的自由基清除能力。建立了成熟的局部缺血小鸡心肌细胞模型(1 h /再灌注)(3 h),以评估类黄酮诱导的针对慢性治疗(预处理72 h,通过局部缺血/再灌注)的缺血/再灌注损伤的保护作用(在缺血/再灌注期间或仅在再灌注期间)。通过碘化丙啶评估细胞活力。儿茶素对DPPH的清除作用最为显着,其次是原花青素B2,黄e素,黄and素和Wogonin。同样,儿茶素对超氧化物的清除作用最为显着,其次是黄ical素,原花青素B2和黄ical素。对于羟基自由基,只有黄ical素具有明显的清除能力(ESR信号降低> 50%)。对于心肌细胞研究,在慢性治疗方案中,除了类黄精以外,所有类黄酮均显示出抗缺血/再灌注损伤的保护作用。当仅在局部缺血/再灌注期间施用类黄酮时,黄ical素,原花青素B2和儿茶素可显着降低细胞死亡。如果仅在再灌注时施用类黄酮,则只有黄ical素和原花青素B2具有保护作用,且疗效较差。黄酮类化合物具有特定但不同的自由基清除能力,与治疗时机结合,会影响其对缺血/再灌注心肌细胞的保护作用。

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