In vitro chemical properties and antioxidant potential and in vivo mutagenic activity of honey-sweetened cashew apple nectar (HSCAN), a beverage produced from the cashew pseudo-fruit (Anacardium occidentale L.) and of its constituents were assessed. Analytical procedures were carried out to investigate the honey used in the HSCAN preparation, and the results observed are in accordance with Brazilian legal regulations, except for diastase number. HSCAN and pulp were investigated for ascorbic acid, carotenoid, anthocyanin and total phenolic contents, and both showed high acid ascorbic concentrations. Antioxidant capacity using 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) and/or β-carotene/linoleic acid systems were applied and demonstrated a weak antioxidant capacity of honey and HSCAN, but cashew apple pulp demonstrated high antioxidant capacity. A weakly positive mutagenic effect of cashew pulp 20% was observed using the somatic mutation and recombination test (SMART) in Drosophila melanogaster only in the high-bioactivation (HB) cross. On the contrary, HSCAN was not mutagenic in both standard and high bioactivation crosses. HSCAN exhibited slight antioxidant activity, which could be associated with the high amount of ascorbic acid found in the samples evaluated. The beverage prepared did not induce DNA damage in somatic cells of D. melanogaster, which means that it is neither mutagenic nor recombinagenic in this test system.

译文

:对蜂蜜加糖的腰果苹果花蜜(HSCAN)的体外化学性质,抗氧化剂潜力和体内诱变活性进行了评估,该饮料是由腰果假果(Anacardium occidentale L.)生产的饮料及其成分。进行了分析程序以调查HSCAN制剂中使用的蜂蜜,观察到的结果符合巴西法律法规,但发乳酶数量除外。对HSCAN和纸浆进行了抗坏血酸,类胡萝卜素,花色苷和总酚含量的调查,结果均显示出较高的酸性抗坏血酸浓度。应用了使用2,2-二苯基-1-吡啶并肼基(DPPH)和/或β-胡萝卜素/亚油酸体系的抗氧化能力,证明了蜂蜜和HSCAN的抗氧化能力较弱,但腰果苹果浆显示出较高的抗氧化能力。仅在高生物激活(HB)杂交中,使用果蝇果蝇的体细胞突变和重组试验(SMART)观察到了腰果果肉20%的弱阳性诱变作用。相反,HSCAN在标准和高生物激活杂交中均不致突变。 HSCAN表现出轻微的抗氧化活性,这可能与所评估样品中发现的大量抗坏血酸有关。所制备的饮料不会在黑腹果蝇的体细胞中引起DNA损伤,这意味着该饮料在该测试系统中既不诱变也不重组。

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