Amphibian skin secretions are known to contain numerous peptides with a large array of biological activities. Bombinins are a group of amphibian-derived peptides with broad spectrum antimicrobial activities that have been only identified from the ancient toad species, Bombina. In this study, we described the identification and characterization of a novel bombinin precursor which encoded a bombinin-like peptide (BLP-7) and a novel bombinin H-type peptide (named as Bombinin H-BO) from the skin secretion of Oriental fire-bellied toad, Bombina orientalis. The primary structures of both mature peptides were determined by combinations of molecular cloning of peptide precursor-encoding cDNAs and mass spectrometry techniques. Secondary structure prediction revealed that both peptides had cationic amphipathic α-helical structural features. The synthetic replicate of BLP-7 displayed more potent antimicrobial activity than Bombinin H-BO against Gram-positive and Gram-negative bacteria and yeast. Also, in vitro antitumour assay showed that both peptides possessed obvious antiproliferative activity on three human hepatoma cells (Hep G2/SK-HEP-1/Huh7) at the non-toxic doses. These results indicate the peptide family of bombinins could be a potential source of drug candidates for anti-infection and anticancer therapy.

译文

已知两栖动物的皮肤分泌物中含有许多具有多种生物活性的肽。 Bombinins是一组具有广谱抗菌活性的两栖动物衍生肽,仅从古老的蟾蜍物种Bombina中鉴定出来。在这项研究中,我们描述了一种从东方火的皮肤分泌物中鉴定出一种新的蛙新蛋白前体的鉴定和表征,该前体编码了一种蛙新蛋白样肽(BLP-7)和一种新颖的蛙新蛋白H型肽(称为蛙蛋白H-BO)腹蟾蜍,Bombina Orientalis。通过结合肽前体编码cDNA的分子克隆和质谱技术确定两种成熟肽的一级结构。二级结构预测表明,两种肽均具有阳离子两亲性α-螺旋结构特征。与Bombinin H-BO相比,BLP-7的合成复制品对革兰氏阳性和革兰氏阴性细菌和酵母菌显示出更强的抗菌活性。同样,体外抗肿瘤试验表明,两种肽均以无毒剂量对三种人肝癌细胞(Hep G2 / SK-HEP-1 / Huh7)具有明显的抗增殖活性。这些结果表明,蛙皮素的肽家族可能是抗感染和抗癌治疗候选药物的潜在来源。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录