The objective of this study was to explore a paradigm that would allow a temporary deprivation of whisker information lasting for a few hours. An additional requirement was to be non-invasive in order to be usable in awake chronically implanted rats without inducing stress. With that aim, electrophysiological recordings from the barrel cortex of anesthetized rats were obtained. The pressure of an air-puff (5-10 ms) delivered to the whiskers was adjusted to evoke a consistent response of around 100 microV (extracellular) or approximately 5 mV (intracellular) in the contralateral cortex. Lidocaine was then locally applied in different forms (cream, local injection, aerosol, drops) and concentrations (2-10%) to the base of the whiskers. The stimulus-induced response was monitored once every 5s for several hours (3-6h) in order to characterize its course of action. Local injection of lidocaine induced the fastest and most complete blockage, but was ruled out for being invasive. Out of the remaining forms of application, a lidocaine drop (0.4 ml, 10%) to the base of the whiskers was found to induce a reliable blockage (to an average 9% the original response). The maximum effect was reached after 150-200 min, and the response was totally recovered approximately 300 min after lidocaine application. This characterization should be useful to induce an efficient, short term and reversible blockage of whisker sensory transmission in both anesthetized and awake preparations, while not causing stress in an awake animal.

译文

:这项研究的目的是探索一种范式,该范式可以暂时剥夺持续数小时的晶须信息。另一个要求是非侵入性的,以便可以在清醒的慢性植入大鼠中使用而不会引起压力。为此,从麻醉大鼠的桶状皮层获得了电生理记录。调节送至晶须的吹气压力(5-10毫秒),以引起对侧皮质中约100 microV(细胞外)或约5 mV(细胞内)的一致反应。然后将利多卡因以不同形式(乳膏,局部注射,气雾剂,滴剂)和浓度(2-10%)局部施用到胡须的基底上。每5s监测一次刺激诱导的反应,持续数小时(3-6h),以表征其作用过程。局部注射利多卡因可引起最快和最完全的阻塞,但由于具有侵入性而被排除在外。在其余的施用形式中,发现在晶须底部滴加利多卡因(0.4 ml,10%)可引起可靠的阻塞(平均原始响应的9%)。 150-200分钟后达到最大效果,利多卡因施用后约300分钟,反应完全恢复。此特征对于在麻醉和清醒的制剂中诱导晶须感觉传递的有效,短期和可逆性阻滞,而不会在清醒的动物中引起压力方面应该是有用的。

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