Auto-anti-idiotypic mechanisms can regulate the protective immune response against Schistosoma mansoni. Anti-idiotypic responses were stimulated by immunization of mice either with nonspecifically induced lymphoblasts, produced with Con A, or with Ag-induced lymphoblasts bearing specific idiotypic receptors. The effect of the induced anti-idiotypic response upon clonotypic cellular reactivity was assessed in vitro through the suppression of antigen-mediated blast transformation by cloned T cells and in vivo by suppression of resistance to S. mansoni and delayed-type hypersensitivity responses against specific Ag. Differential regulation of humoral immune responses was studied at the levels of specific epitopic recognition, the expression of specific Id, and the production of anti-idiotypic responses directed against mAb bearing specific Id. Anti-idiotypic sensitization resulted in variable (10 to 90%) suppression of the immune response to discrete antigenic epitopes, the expression of specific idiotypic phenotypes, and anti-idiotypic, antiparatopic responses against T cell clonotypes and antibody idiotypic phenotypes. In vitro admixture and in vivo challenge studies resulted in consonant differential suppression. Thus idiotypic regulation can mold the fine specificities of the protective immune response to S. mansoni at the clonal level and may provide an approach to optimize the expression and assessment of resistance.

译文

自动抗独特型机制可以调节针对曼氏血吸虫的保护性免疫反应。通过用Con A产生的非特异性诱导的淋巴母细胞,或用Ag诱导的携带特定独特型受体的淋巴母细胞免疫小鼠,可以刺激抗独特型应答。在体外通过抑制克隆的T细胞对抗原介导的胚泡转化的抑制来评估诱导的抗独特型反应对克隆型细胞反应性的影响,并在体内通过抑制对曼氏沙门氏菌的抗性和针对特定Ag的迟发型超敏反应来评估体内诱导的抗独特型反应的作用。 。研究了在特定表位识别,特定Id的表达水平以及针对带有特定Id的mAb的抗独特型反应水平上对体液免疫反应的差异调节。抗独特型致敏作用导致对离散抗原表位的免疫反应,特异性独特型表型的表达以及针对T细胞克隆型和抗体独特型表型的抗独特型,抗副反应的变化受到抑制(10%至90%)。体外混合和体内攻击研究导致辅音差异抑制。因此,独特型调节可以在克隆水平上塑造对曼氏链球菌的保护性免疫应答的优良特异性,并且可以提供优化抗性表达和评估的方法。

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