Effects of chlorobenzene-induced alteration in glutathione levels on cysteine metabolism in rat liver were investigated. Male Wistar rats were intraperitoneally injected with chlorobenzene (0.2, 0.5, 1.0 or 2.0 mmol/kg body weight). Both hepatic glutathione and cysteine levels were dose-dependently decreased by the chlorobenzene 6 h after the injection. However, at 24 h, the glutathione in the rats with chlorobenzene increased significantly as compared to that in the rats without chlorobenzene. Concomitant to the elevation in glutathione levels, hepatic glutathione synthesis activities were increased by the chlorobenzene by 68-111%. On the other hand, no significant difference between the rats with and without chlorobenzene was observed as regards cysteine levels at 24 h. Hepatic glutamate, glycine, methionine and serine levels were unaltered but hepatic taurine levels were significantly decreased by the chlorobenzene at both 6 and 24 h. Chlorobenzene administration had no effect on hepatic cystathionine synthase and cystathionase activities. These results indicate that a transient loss of hepatic glutathione, caused by the administration of chlorobenzene, resulted in an acceleration of glutathione synthesis and an increase of cysteine demand in the liver.

译文

:研究了氯苯诱导的谷胱甘肽水平改变对大鼠肝脏半胱氨酸代谢的影响。给雄性Wistar大鼠腹膜内注射氯苯(0.2、0.5、1.0或2.0mmol / kg体重)。注射后6小时,氯苯使肝谷胱甘肽和半胱氨酸水平均呈剂量依赖性降低。然而,在24小时时,与不含氯苯的大鼠相比,含氯苯的大鼠中的谷胱甘肽显着增加。伴随谷胱甘肽水平升高,氯苯使肝谷胱甘肽合成活性提高了68-111%。另一方面,在有和没有氯苯的大鼠之间,在24小时的半胱氨酸水平上没有观察到显着差异。肝谷氨酸,甘氨酸,蛋氨酸和丝氨酸水平未改变,但在6小时和24小时时,氯苯可显着降低肝牛磺酸水平。施用氯苯对肝脏胱硫醚合酶和胱硫醚酶活性没有影响。这些结果表明,由于施用氯苯而引起的肝性谷胱甘肽的瞬时损失导致了谷胱甘肽合成的加速和肝脏中半胱氨酸需求的增加。

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