Peptidylarginine deiminase 6 (PAD6) is an enzyme that is uniquely expressed in male and female germ cells. To study the function of this enzyme in vivo we generated mice deficient for PAD6. Here we show that inactivation of the PAD6 gene in mice leads to female infertility whereas male fertility is not affected. The absence of the PAD6 protein and consequently absence of citrullination activity in oocytes results in dispersal of the cytoskeletal sheets in oocytes, indicating an essential role of these germ cell-specific structures in zygote/embryo development. PAD6 deficient mice do not show any other overt phenotype. Thus, we identify citrullination as a new regulator of fertility.

译文

:肽基精氨酸脱亚氨酶6(PAD6)是一种在雄性和雌性生殖细胞中独特表达的酶。为了研究该酶在体内的功能,我们产生了PAD6缺陷的小鼠。在这里,我们显示,小鼠中PAD6基因的失活导致女性不育,而男性不育不受影响。卵母细胞中缺乏PAD6蛋白,因此缺乏瓜氨酸化活性,导致卵母细胞中细胞骨架片的分散,表明这些生殖细胞特异性结构在合子/胚胎发育中起着至关重要的作用。 PAD6缺陷的小鼠没有显示任何其他明显的表型。因此,我们确定瓜氨酸化是生育的新调节剂。

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