The prevalence of the metabolic syndrome (MS) is rapidly increasing all over the world. Consequently, there is an urgent need for more effective intervention strategies. Both animal and human studies indicate that lipid oversupply to skeletal muscle can result in insulin resistance, which is one of the characteristics of the MS. C57BL/6J mice were fed a low-fat (10 kcal%) palm oil diet or a high-fat (45 kcal%; HF) palm oil diet for 3 or 28 days. By combining transcriptomics with protein and lipid analyses we aimed to better understand the molecular events underlying the early onset of the MS. Short-term HF feeding led to altered expression levels of genes involved in a variety of biological processes including morphogenesis, energy metabolism, lipogenesis, and immune function. Protein analysis showed increased levels of the myosin heavy chain, slow fiber type protein, and the complexes I, II, III, IV, and V of the oxidative phosphorylation. Furthermore, we observed that the main mitochondrial membrane phospholipids, phosphatidylcholine and phosphatidylethanolamine, contained more saturated fatty acids. Altogether, these results point to a morphological as well as a metabolic adaptation by promoting a more oxidative fiber type. We hypothesize that after this early positive adaptation, a continued transcriptional downregulation of genes involved in oxidative phosphorylation will result in decreased oxidative capacity at a later stage. Together with increased saturation of phospholipids of the mitochondrial membrane this can result in decreased mitochondrial function, which is a hallmark observed in insulin resistance and Type 2 diabetes.

译文

:代谢综合征(MS)的患病率在世界范围内迅速增加。因此,迫切需要更有效的干预策略。动物和人体研究均表明,脂质过度供应至骨骼肌可导致胰岛素抵抗,这是MS的特征之一。给C57BL / 6J小鼠饲喂低脂(10 kcal%)棕榈油饮食或高脂(45 kcal%; HF)棕榈油饮食3或28天。通过将转录组学与蛋白质和脂质分析相结合,我们旨在更好地了解MS早期发作的分子事件。短期HF喂养导致涉及多种生物学过程的基因表达水平发生改变,这些生物学过程包括形态发生,能量代谢,脂肪形成和免疫功能。蛋白质分析显示,肌球蛋白重链,慢纤维型蛋白质以及氧化磷酸化的复合物I,II,III,IV和V的水平增加。此外,我们观察到主要的线粒体膜磷脂,磷脂酰胆碱和磷脂酰乙醇胺,含有更多的饱和脂肪酸。总而言之,这些结果通过促进更具氧化性的纤维类型而指向形态和代谢适应。我们假设,在这种早期的积极适应之后,与氧化磷酸化有关的基因的持续转录下调将在以后的阶段导致氧化能力下降。连同线粒体膜磷脂饱和度的增加,这可导致线粒体功能降低,这是在胰岛素抵抗和2型糖尿病中观察到的标志。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录