PHF7 exhibits male-specific expression in early germ cells, germline stem cells and spermatogonia in insects, and its expression promotes spermatogenesis in germ cells when they are present in a male soma. However, the influence of male-specific PHF7 on female reproductive biology via mating remains unclear. Thus, we investigated the potential impacts of male PHF7, existed in seminal fluid of Nilaparvata lugens (NlPHF7), on fecundity and population growth via mating. Our results revealed that suppressing male NlPHF7 expression by RNAi led to decreases in body weight, soluble accessory gland protein content, arginine content, and reproductive organ development in males, resulting in significant reduction of oviposition periods and fecundity in females, and significant decrease in body weight, fat body and ovarian protein content, yeast-like symbionts abundance, ovarian development and vitellogenin gene expression in their female mating partners. Similarly, suppression of NlPHF7 expression in males mated with the control female reduced population growth and egg hatching rate, but did not influence gender ratio. We infer that NlPHF7 play a role important in stimulating female fecundity via mating. This study provides valuable information by identifying a potentially effective target gene for managing BPH population through RNAi.

译文

:PHF7在昆虫的早期生殖细胞,生殖系干细胞和精原细胞中表现出雄性特异性表达,当它们存在于雄性体细胞中时,其表达促进生殖细胞中的精子发生。但是,尚不清楚男性特异性PHF7通过交配对女性生殖生物学的影响。因此,我们调查了Nilaparvata lugens(NlPHF7)精液中存在的雄性PHF7通过繁殖对繁殖力和种群增长的潜在影响。我们的研究结果表明,通过RNAi抑制雄性NlPHF7表达可导致男性体重,可溶性附属腺蛋白含量,精氨酸含量和生殖器官发育下降,从而导致雌性产卵周期和生殖力显着减少,而体质显着下降雌性交配伙伴的体重,脂肪体和卵巢蛋白含量,酵母样共生体丰度,卵巢发育和卵黄蛋白原基因表达。类似地,在雄性中抑制NlPHF7表达与对照雌性交配会降低种群增长和卵孵化率,但不影响性别比。我们推断,NlPHF7在通过交配刺激女性生殖力中起重要作用。这项研究通过鉴定潜在有效的靶基因来通过RNA干扰管理BPH种群提供了有价值的信息。

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