Male ICR mice, young (25-days old), mature (3-months old), and old (22 months), were injected with morphine sulfate (10 mg/kg, s.c.) or were implanted with morphine pellets (75 mg). Controls received saline injections or placebo pellets. One hour after injections and 72 h after pellet implantations, the mice were decapitated and striatal regions were removed for the following analyses: calmodulin (CaM) levels via radioimmunoassay and activities of cyclic nucleotide phosphodiesterases, adenylate and guanylate cyclases, and Ca2+, Mg2+-ATPase. Acute morphine treatment produced the following: (1) increases in calmodulin levels in the young and old mice while having no effect on mature levels; (2) increases in activities of guanylate cyclase of mature mice while decreasing those of the old mice; (3) no effects on activity of adenylate cyclase; (4) decreased activity of cyclic AMP-phosphodiesterase in young mice only; (5) decreased activity of Ca2+, Mg2+-ATPase in the old mice only. The only changes found in striata from morphine-tolerant mice when compared with age-matched controls were elevations in cyclic GMP-phosphodiesterase activities in all three age groups. Differences in control values of the three age groups were as follows: CaM levels, mature greater than old greater than young; Ca2+, Mg2+-ATPase activity, old greater than mature-young. The results indicate age-induced changes in cellular regulation and biochemical responses to morphine.

译文

年轻 (25天大),成熟 (3个月大) 和年龄 (22个月大) 的雄性ICR小鼠注射硫酸吗啡 (10 mg/kg,s.c.) 或植入吗啡颗粒 (75 mg)。对照组接受生理盐水注射或安慰剂颗粒。注射后1小时和颗粒植入后72小时,将小鼠斩首并去除纹状体区域进行以下分析: 通过放射免疫测定法测定钙调蛋白 (CaM) 水平以及环核苷酸磷酸二酯酶,腺苷酸和鸟苷酸环化酶以及Ca2的活性,mg2-atpase。急性吗啡处理产生以下结果 :( 1) 年轻和老年小鼠的钙调蛋白水平升高,而对成熟水平无影响; (2) 成熟小鼠鸟苷酸环化酶活性增加,而老年小鼠的鸟苷酸环化酶活性降低; (3) 对腺苷酸环化酶活性无影响; (4) 仅在年轻小鼠中降低环AMP-磷酸二酯酶的活性; (5) 仅在老年小鼠中降低Ca2,Mg2-ATPase的活性。与年龄匹配的对照组相比,吗啡耐受小鼠的纹状体中发现的唯一变化是所有三个年龄组的环GMP-磷酸二酯酶活性均升高。三个年龄组的对照值差异如下: CaM水平,成熟大于年龄大于年轻; Ca2,mg2-atpase活性,老大于成熟-年轻。结果表明,年龄引起的细胞调节和对吗啡的生化反应的变化。

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