A Gram-negative, rod shaped, motile bacterium, was isolated from a marine solar saltern sample collected from Kakinada, India. Strain AK2(T) was determined to be positive for nitrate reduction, catalase, Ala-Phe-Pro-arylamidase, β-galactosidase, β-N-acetylglucosaminidase, β-glucosidase, β-xylosidase, α-glucosidase, α-galactosidase and phosphatase activities, hydrolysis of aesculin, Tween 20/40/60/80 and urea. It was determined to be negative for oxidase, lysine decarboxylase and ornithine decarboxylase activities and could not hydrolyze agar, casein, gelatin and starch. The predominant fatty acids were identified as iso-C(15:0) (28.2 %), anteiso-C(15:0) (23.2 %), iso-C(13:0) (19.9 %) and iso-C(15:0) 3-OH (13.9 %). Strain AK2(T) was found to contain menaquinone with seven isoprene units (MK-7) as the sole respiratory quinone and phosphatidylethanolamine, one unidentified phospholipid and three unidentified lipids as polar lipids. The 16S rRNA gene sequence analysis indicated the strain AK2(T) as a member of the genus Marinilabilia and is closely related to Marinilabilia salmonicolor with pair-wise sequence similarity of 98.2 %. Phylogenetic analysis of 16S rRNA gene revealed that the strain AK2(T) clustered with M. salmonicolor. However, DNA-DNA hybridization with M. salmonicolor JCM 21150(T) showed a relatedness of 48 ± 0.5 % with respect to strain AK2(T). The DNA G+C content of the strain was determined to be 40.2 mol%. Based on the phenotypic characteristics and phylogenetic inference, it is proposed that the strain AK2(T) represents a novel species of the genus Marinilabilia, for which the name Marinilabilia nitratireducens sp. nov. is proposed. The type strain of M. nitratireducens sp. nov. is AK2(T) (= MTCC 11402(T) = JCM 17679(T)).

译文

:从印度Kakinada收集的海洋太阳能盐分离器样本中分离出革兰氏阴性,棒状,能动细菌。确定菌株AK2(T)对硝酸盐还原,过氧化氢酶,Ala-Phe-前芳酰胺酶,β-半乳糖苷酶,β-N-乙酰氨基葡糖苷酶,β-葡萄糖苷酶,β-木糖苷酶,α-葡萄糖苷酶,α-半乳糖苷酶和磷酸酶活性,七叶皂苷,Tween 20/40/60/80和尿素的水解。它被确定为氧化酶,赖氨酸脱羧酶和鸟氨酸脱羧酶活性阴性,并且不能水解琼脂,酪蛋白,明胶和淀粉。主要脂肪酸被鉴定为iso-C(15:0)(28.2%),anteiso-C(15:0)(23.2%),iso-C(13:0)(19.9%)和iso-C( 15:0)3-OH(13.9%)。发现菌株AK2(T)含有具有七个异戊二烯单元(MK-7)的甲基萘醌作为唯一的呼吸醌和磷脂酰乙醇胺,一个未确定的磷脂和三个未确定的脂质作为极性脂质。 16S rRNA基因序列分析表明,菌株AK2(T)是马里尼布拉马氏菌属的成员,与马里尼拉鲑鱼色密切相关,成对序列相似性为98.2%。 16S rRNA基因的系统发育分析表明,菌株AK2(T)与沙门氏菌成簇。但是,与鲑鱼支原体JCM 21150(T)的DNA-DNA杂交显示出与菌株AK2(T)的相关性为48±0.5%。测得该菌株的DNA G C含量为40.2mol%。根据表型特征和系统发育推论,建议菌株AK2(T)代表Marinilabilia属的一个新种,其名称为Marinilabilianitroatireducens sp。十一月被提议。硝化分枝杆菌的类型菌株。十一月是AK2(T)(= MTCC 11402(T)= JCM 17679(T))。

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