An experimental system has been developed which allows the identification of intermediates in the abscisic acid (ABA) signal transduction pathway leading to desiccation tolerance in plants. Desiccation tolerance in callus of the resurrection plant Craterostigma plantagineum is mediated via the plant hormone ABA, which induces the expression of gene products related to desiccation tolerance. Based on T-DNA activation tagging, a gene (CDT-1) was isolated which encodes a signalling molecule in the ABA transduction pathway. Constitutive overexpression of CDT-1 leads to desiccation tolerance in the absence of ABA and to the constitutive expression of characteristic transcripts. CDT-1 represents a novel gene with unusual features in its primary sequence. The CDT-1 gene resembles in several features SINE retrotransposons. Mechanisms by which CDT-1 activates the pathway could be via a regulatory RNA or via a short polypeptide.

译文

已经开发了一种实验系统,该系统允许鉴定脱落酸(ABA)信号转导途径中的中间体,导致植物中的脱水耐受性。复活植物Craterostigma plantagineum愈伤组织中的干燥耐性是通过植物激素ABA介导的,ABA诱导了与干燥耐性有关的基因产物的表达。基于T-DNA激活标记,分离了一个基因(CDT-1),该基因在ABA转导途径中编码一个信号分子。 CDT-1的组成型过表达会导致在不存在ABA的情况下产生脱水耐受性,并导致特征性转录本的组成型表达。 CDT-1代表一个在其主要序列中具有异常特征的新基因。 CDT-1基因在几个特征上类似于SINE逆转座子。 CDT-1激活该途径的机制可能是通过调控RNA或通过短多肽。

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