Proteins encoded by MADS-box genes are important transcription factors involved in the regulation of flowering plant growth and development. Currently, no systematic information exists regarding the MADS-box family in the important tropical fruit banana. Ninety-six MADS-box genes were identified from the banana (Pahang) A genome. Phylogenetic analysis indicated that Musa acuminata MCM1-AGAMOUS- DEFICIENS-SRF (MaMADS) could be divided into MIKCc, MIKC*, Mα/β and Mγ groups. MIKCc could be further divided into 11 subfamilies, which was further supported by conserved motif and gene structure analyses. Transcriptome analysis on the Feng Jiao (FJ) and BaXi Jiao (BX) banana cultivars revealed that MaMADS genes are differentially expressed in various organs, at different fruit development and ripening stages, indicating the involvement of these genes in fruit development and ripening processes. Interactive network analysis indicated that MaMADS24 and 49 not only interacted with MaMADS proteins themselves, but also interacted with hormone-response proteins, ethylene signal transduction and biosynthesis-related proteins, starch biosynthesis proteins and metabolism-related proteins. This systematic analysis identified candidate MaMADS genes related to fruit development and ripening for further functional characterization in plants, and also provided new insights into the transcriptional regulation of MaMADS genes, facilitating the future genetic manipulation of MADS-mediated fruit development and ripening.

译文

MADS-box基因编码的蛋白质是重要的转录因子,参与开花植物生长发育的调控。目前,在重要的热带水果香蕉中,尚无有关MADS盒家族的系统信息。从香蕉(彭亨州)A基因组中鉴定出96个MADS-box基因。系统发育分析表明,Musus acuminata MCM1-AGAMOUS-DEFICIENS-SRF(MaMADS)可以分为MIKCc,MIKC *,Mα/β和Mγ组。 MIKCc可以进一步分为11个亚家族,这由保守的基序和基因结构分析进一步支持。凤角(FJ)和八喜角(BX)香蕉品种的转录组分析表明,MaMADS基因在不同器官中处于不同的果实发育和成熟阶段差异表达,表明这些基因参与了果实发育和成熟过程。交互网络分析表明,MaMADS24和49不仅与MaMADS蛋白本身相互作用,而且还与激素响应蛋白,乙烯信号转导和生物合成相关蛋白,淀粉生物合成蛋白和代谢相关蛋白相互作用。这项系统分析确定了与果实发育和成熟有关的候选MaMADS基因,以进一步在植物中进行功能表征,也为MaMADS基因的转录调控提供了新见识,从而促进了MADS介导的果实发育和成熟的未来遗传调控。

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