Methionine adenosyltransferase (MAT) is involved in folate-mediated one-carbon metabolism, which is essential for preimplantation embryos in terms of both short-term periconceptional development and long-term phenotypic programming beyond the periconceptional period. Here, our immunofluorescence analysis of bovine oocytes and preimplantation embryos revealed the consistent expression of MAT2A (the catalytic subunit of the ubiquitously expressed-type of MAT isozyme) during this period. Addition of the MAT2A inhibitor FIDAS to the culture media of bovine preimplantation embryos reduced their blastocyst development, revealing the particular importance of MAT2A in successful blastocyst development. Exploration of MAT2A-associated genomic regions in bovine blastocysts using chromatin immunoprecipitation and sequencing (ChIP-seq) identified candidate MAT2A-associated genes implicated not only in short-term periconceptional embryo development, but also in long-term phenotypic programming during this period in terms of growth, metabolism, and immune functions. These results suggest the critical involvement of MAT2A in the periconceptional period in life-long programming of health and disease as well as successful preimplantation development.

译文

:蛋氨酸腺苷基转移酶(MAT)参与叶酸介导的一碳代谢,这对于植入前的胚胎而言,无论是短期的受孕发育还是经过长期的表型编程,都是必不可少的。在这里,我们对牛卵母细胞和植入前胚胎的免疫荧光分析揭示了在此期间MAT2A(MAT酶的普遍表达型的催化亚基)的一致表达。在牛植入前胚胎的培养基中添加MAT2A抑制剂FIDAS可减少其胚泡发育,从而揭示MAT2A在成功的胚泡发育中特别重要。利用染色质免疫沉淀和测序(ChIP-seq)探索牛胚泡中与MAT2A相关的基因组区域,确定了与MAT2A相关的候选基因,不仅与短期围产期胚胎发育有关,而且与这一时期的长期表型编程有关。生长,新陈代谢和免疫功能。这些结果表明,MAT2A在整个生命周期的健康和疾病规划以及成功的植入前发展过程中,都处于围孕期的关键时期。

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