Background:The sorghum stem can be divided into the pith and rind parts with obvious differences in cell type and chemical composition, thus arising the different recalcitrance to enzyme hydrolysis and demand for different pretreatment conditions. The introduction of organic solvents in the pretreatment can reduce over-degradation of cellulose and hemicellulose, but significance of organic solvent addition in pretreatment of different parts of sorghum stem is still unclear. Valorization of each component is critical for economy of sorghum biorefinery. Therefore, in this study, NaOH-ethanol pretreatment condition for different parts of the sorghum stem was optimized to maximize p-coumaric acid release and total reducing sugar recovery. Result:Ethanol addition improved p-coumaric acid release and delignification efficiency, but significantly reduced hemicellulose deconstruction in NaOH-ethanol pretreatment. Optimization using the response surface methodology revealed that the pith, rind and whole stem require different NaOH-ethanol pretreatment conditions for maximal p-coumaric acid release and xylan preservation. By respective optimal NaOH-ethanol pretreatment, the p-coumaric acid release yields reached 94.07%, 97.24% and 95.05% from pith, rind and whole stem, which increased by 8.16%, 8.38% and 8.39% compared to those of NaOH-pretreated samples. The xylan recoveries of pith, rind and whole stem reached 76.80%, 88.46% and 85.01%, respectively, which increased by 47.75%, 15.11% and 35.97% compared to NaOH pretreatment. Adding xylanase significantly enhanced the enzymatic saccharification of pretreated residues. The total reducing sugar yields after respective optimal NaOH-ethanol pretreatment and enzymatic hydrolysis reached 84.06%, 82.29% and 84.09% for pith, rind and whole stem, respectively, which increased by 29.56%, 23.67% and 25.56% compared to those of NaOH-pretreated samples. Considering the separation cost of the different stem parts, whole sorghum stem can be directly used as feedstock in industrial biorefinery. Conclusion:These results indicated that NaOH-ethanol is effective for the efficient fractionation and pretreatment of sorghum biomass. This work will help to understand the differences of different parts of sorghum stem under NaOH-ethanol pretreatment, thereby improving the full-component utilization of sorghum stem.

译文

背景:高粱茎可分为髓部和果皮部分,其细胞类型和化学组成明显不同,因此对酶水解的抵抗力不同,对不同预处理条件的需求也不同。在预处理中引入有机溶剂可以减少纤维素和半纤维素的过度降解,但是在预处理高粱茎杆的不同部位中添加有机溶剂的意义仍然不清楚。每个成分的均价对于高粱生物精炼厂的经济性至关重要。因此,在这项研究中,针对高粱茎不同部位的NaOH-乙醇预处理条件进行了优化,以最大程度地释放对香豆酸并降低糖的总回收率。
结果:乙醇的添加改善了对香豆酸的释放和去木质素的效率,但显着减少了NaOH-乙醇预处理中半纤维素的解构。使用响应面方法进行的优化表明,髓,果皮和整个茎需要不同的NaOH-乙醇预处理条件,以最大程度地释放对香豆酸和保留木聚糖。通过分别进行最佳的NaOH-乙醇预处理,对香豆酸从髓,果皮和整个茎中的释放率分别达到94.07%,97.24%和95.05%,比NaOH预处理的分别提高了8.16%,8.38%和8.39%。样品。髓,果皮和整个茎的木聚糖回收率分别达到76.80%,88.46%和85.01%,与NaOH预处理相比增加了47.75%,15.11%和35.97%。添加木聚糖酶显着增强了预处理残基的酶促糖化作用。分别进行了最佳的NaOH-乙醇预处理和酶水解后,髓,果皮和整个茎的总还原糖产量分别达到84.06%,82.29%和84.09%,与NaOH相比分别增加了29.56%,23.67%和25.56%。 -预处理的样品。考虑到不同茎部分的分离成本,整个高粱茎可以直接用作工业生物精炼厂的原料。
结论:这些结果表明NaOH-乙醇对高粱生物质的有效分馏和预处理是有效的。这项工作将有助于了解在NaOH-乙醇预处理下高粱茎秆不同部位的差异,从而提高了高粱茎秆的全成分利用率。

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